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anti-Human TUBB3 antibody for Immunocytochemistry

Recommended TUBB3 Antibody (supplied by: Log in to see )

Antigen
Tubulin, Beta, 3 (TUBB3) Antibodies
  • tubb3
  • TUBB3
  • TUBB4
  • CDCBM
  • CFEOM3A
  • beta-4
  • 3200002H15Rik
  • M(beta)3
  • M(beta)6
  • AI325297
  • M(beta)4
  • Tubb
  • Tubb4
  • tubulin, beta 3 class III
  • beta-tubulin 3
  • similar to tubulin, beta 3
  • tubulin, beta, 4
  • tubulin, beta 3
  • beta tubulin3
  • tubulin, beta 2C
  • tubulin, beta 4A class IVA
  • tubb3
  • TUBB3
  • tub3
  • TUBB2C
  • Tubb3
  • Tubb4a
Epitope
AA 443-450
50
44
21
13
12
6
5
4
4
4
3
3
3
2
2
1
1
1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
216
86
77
20
15
10
7
4
3
2
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
129
119
16
14
1
Clonality
Polyclonal
Conjugate
This TUBB3 antibody is un-conjugated
26
14
13
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
228
102
85
66
60
57
51
16
13
11
11
8
6
2
2
2
2
2
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN1742553
$ 358.05
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.37591 ABIN969447 ICC FACS ELISA WB Mouse IgG1 Log in to see 2-00E-09 2
26.37591 ABIN1742554 ICC IP IHC WB Guinea Pig AA 443-450 Log in to see Polyclonal 3
26.37591 ABIN1846608 ICC FACS IF ELISA WB Mouse IgG1 Log in to see
1 ABIN574424 ICC FACS IHC (p) WB Mouse IgG1 C-Term Log in to see TU-20 6
1 ABIN151159 ICC IF IHC IHC (wm) WB Chicken Log in to see Polyclonal 5
1 ABIN4284542 ELISA FACS ICC IF SimWes WB Mouse IgG1 Log in to see 2E9 1
1 ABIN625871 ICC IHC (p) WB Mouse IgG2a AA 436-450 Log in to see
1 ABIN626158 ICC IHC (p) WB Chicken IgY Log in to see Polyclonal
1 ABIN2471059 ICC FACS IHC WB Mouse IgG1 N-Term Log in to see TU-20
1 ABIN4284545 FACS ICC IF IHC IP WB Mouse IgG2a Log in to see 2G10
1 ABIN199726 ICC IHC (fro) IHC (p) IP ELISA WB Mouse IgG1 AA 443-450 Log in to see
1 ABIN221030 ICC IHC (p) IP ELISA WB Mouse IgG1 AA 443-450 Log in to see
1 ABIN1724161 ICC FACS IHC (p) WB Mouse IgG1 N-Term Log in to see TU-20
1 ABIN2471139 ICC IHC WB Mouse IgG2a AA 436-450 Log in to see
1 ABIN1723293 ICC IHC (p) WB Mouse IgG2a AA 436-450 Log in to see
1 ABIN2869438 ELISA FACS ICC WB Mouse IgG1 Log in to see 2E9
1 ABIN3021273 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2466915 ICC IHC WB Chicken IgY Log in to see Polyclonal
1 ABIN1860885 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 222-438 Log in to see Polyclonal
1 ABIN2801452 ICC IP WB Mouse IgG1, kappa C-Term Log in to see Tuj-1-TubIII-4G3

Top referenced anti-TUBB3 antibody for Immunocytochemistry

Similar anti-TUBB3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 9 Antibodies 11 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 8 Antibodies 24 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies
Immunohistochemistry (Whole Mount) (IHC (wm)) 2 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 61 Antibodies

General

Antigen Tubulin, Beta, 3 (TUBB3) Antibodies
Epitope AA 443-450
(50), (44), (21), (13), (12), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Reactivity Human, Rat (Rattus), Mouse (Murine)
(216), (86), (77), (20), (15), (10), (7), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(129), (119), (16), (14), (1)
Clonality
Polyclonal
Conjugate This TUBB3 antibody is un-conjugated
(26), (14), (13), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(228), (102), (85), (66), (60), (57), (51), (16), (13), (11), (11), (8), (6), (2), (2), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-TUBB3 Antibody

Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN1742553) Images
Specificity Specific for beta3-tubulin.
Purification antiserum
Immunogen Synthetic peptide (aa 443-450 of mouse beta3-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.

Target Details TUBB3

Product Details anti-TUBB3 Antibody Application Details Handling References for anti-TUBB3 Antibody (ABIN1742553) Images back to top
Antigen
Alternative Name beta3-Tubulin (TUBB3) (TUBB3 Antibody Abstract)
Background Synonyms: TuJ1
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB3 Antibody Target Details TUBB3 Handling References for anti-TUBB3 Antibody (ABIN1742553) Images back to top
Application Notes WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 1000 up to 1 : 5000
IHC: 1 : 200 up to 1 : 500
Restrictions For Research Use only

Handling

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details References for anti-TUBB3 Antibody (ABIN1742553) Images back to top
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-TUBB3 Antibody (ABIN1742553)

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling Images back to top
Product cited in:

Kaczor, Rakus, Mozrzymas: "Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes." in: Frontiers in cellular neuroscience, Vol. 9, pp. 120, 2015

Images

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN1742553) back to top
Supplier Images
Western Blotting (WB) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 443-450) (ABIN1742553) anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody
Immunocytochemistry (ICC) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 443-450) (ABIN1742553) anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 443-450) (ABIN1742553) anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (Image 3)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 443-450) (ABIN1742553) Indirect immunofluorescence labeling of PFA fixed, paraffin embedded mouse brain sect...
'Independent Validation' Badge
Lot Number 302302/3
Method validated Western Blotting
Positive Control Mouse brain
Negative Control Mouse heart, HeLa cells, A431 cells
Notes A band was observed in the positive controls at the expected size (~50 kDa), which is lower in the negative controls.
Primary Antibody
  • Antigen: Tubulin, Beta, 3 (TUBB3) (AA 443-450)
  • Catalog number: ABIN1742553
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 302302/3
  • Dilution: 1:1000
Secondary Antibody
  • Antibody: Donkey anti-Rabbit IgG Antibody (HRP)
  • Catalogue number: Log in to see
  • Supplier: Log in to see
  • Lot number: F0613
  • Dilution: 1:10,000
Controls - Lysates were prepared by Kinexus Bioinformatics Corporation following standard protocols and quality controlled for protein integrity on a regular basis.
Protocol
  • 1. Cell/tissue total protein lysates were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 minutes prior to loading.
  • 2. 15 μg of boiled lysate were loaded and resolved on a 12% SDS-polyacrylamide gel.
  • 3. The Precision Plus Protein™ All Blue Prestained Standards from BioRad (161-0373) were used as molecular mass markers.
  • 4. Proteins were transferred onto nitrocellulose membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
  • 5. The immunoblot membrane was blocked in 2.5% skim milk and 1.5% BSA solution in TTBS at room temperature for 60 minutes.
  • 6. The membrane was washed in TTBS twice for 5 minutes each.
  • 7. The membrane was immersed with the protein side up in the antibody solution in TBS and incubated overnight at 4°C with gentle agitation.
  • 8. The membrane was rinsed twice with TTBS.
  • 9. The membrane was washed in TTBS twice for 5 minutes each.
  • 10. The membrane was washed in TTBS once for 15 minutes.
  • 11. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBS for 60 minutes at room temperature with gentle agitation.
  • 12. The membrane was rinsed twice with TTBS.
  • 13. The membrane was washed in TTBS twice for 5 minutes each.
  • 14. The membrane was washed in TTBS once for 15 minutes.
  • 15. Signals were detected by chemiluminescence (ECL). The blot was scanned for 320 seconds.
  • 16. The membrane was rinsed three times with TTBS.
  • 17. Repeated Steps 4-15 with the loading control antibody and its matching secondary antibody. The blot was scanned for 160 seconds.
Experimental Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (ABIN1742553) Figure 1: Western Blot for Tubulin, Beta, 3 (TUBB3). Grey arrowhead indicates the exp...