anti-Mouse (Murine) TUBB3 antibody for Immunofluorescence

Recommended TUBB3 Antibody (supplied by: Log in to see )

Antigen
Tubulin, Beta, 3 (TUBB3) Antibodies
  • tubb3
  • TUBB3
  • TUBB4
  • CDCBM
  • CFEOM3A
  • beta-4
  • 3200002H15Rik
  • M(beta)3
  • M(beta)6
  • AI325297
  • M(beta)4
  • Tubb
  • Tubb4
  • tubulin, beta 3 class III
  • beta-tubulin 3
  • similar to tubulin, beta 3
  • tubulin, beta, 4
  • tubulin, beta 3
  • beta tubulin3
  • tubulin, beta 2C
  • tubulin, beta 4A class IVA
  • tubb3
  • TUBB3
  • tub3
  • TUBB2C
  • Tubb3
  • Tubb4a
Epitope
AA 441-448, C-Term, N-Term
46
44
19
15
13
6
5
4
4
4
4
3
3
2
2
1
1
1
1
1
Reactivity
All Species, Human, Mouse (Murine)
239
116
104
23
19
15
15
8
8
6
6
5
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
Host
Mouse
127
123
19
14
1
Clonality
Monoclonal
Conjugate
This TUBB3 antibody is un-conjugated
24
13
12
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
239
106
78
64
64
54
47
19
13
13
12
8
8
6
2
2
2
Supplier
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Catalog No. ABIN153066
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.16076 ABIN269248 ICC FACS IF IHC (p) IHC WB FITC Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20 10
12.16076 ABIN151159 ICC IF IHC IHC (wm) WB Chicken Log in to see Polyclonal 6
12.16076 ABIN4284545 FACS ICC IF IHC IP WB Mouse IgG2a Log in to see 2G10
1 ABIN781811 IF IHC (p) WB Mouse IgG2a AA 436-450 Log in to see n-a
1 ABIN4284549 FACS ICC IF IHC IHC (p) WB PerCP Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN2477005 IHC (fro) IF IHC (p) WB Mouse IgG1 AA 441-448 Log in to see TU-20 4
1 ABIN2477006 IF FITC Mouse IgG1 Log in to see TU-20 4
1 ABIN784021 IF IHC (p) WB Chicken Ig Log in to see Polyclonal
1 ABIN784019 EIA IF IHC (p) WB Chicken Ig Log in to see Polyclonal
1 ABIN4284546 FACS ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284557 FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284556 FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284547 FACS ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284552 FACS ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284553 FACS ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284551 FACS ICC IF IHC IHC (p) WB DyLight 755 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284559 FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284558 FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN206457 IF FITC Mouse IgG1 C-Term Log in to see
1 ABIN600290 IF IHC (p) WB Mouse IgG1 C-Term Log in to see

Top referenced anti-TUBB3 antibody for Immunofluorescence

Similar anti-TUBB3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 18 Antibodies 78 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Flow Cytometry (FACS) 30 Antibodies
Immunocytochemistry (ICC) 33 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunohistochemistry (Whole Mount) (IHC (wm)) 2 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 93 Antibodies

General

Antigen Tubulin, Beta, 3 (TUBB3) Antibodies
Epitope AA 441-448, C-Term, N-Term
(46), (44), (19), (15), (13), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity All Species, Human, Mouse (Murine)
(239), (116), (104), (23), (19), (15), (15), (8), (8), (6), (6), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(127), (123), (19), (14), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TUBB3 antibody is un-conjugated
(24), (13), (12), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(239), (106), (78), (64), (64), (54), (47), (19), (13), (13), (12), (8), (8), (6), (2), (2), (2)
Pubmed 14 references available
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Product Details anti-TUBB3 Antibody

Target Details TUBB3 Application Details Handling ProductDetails: References for anti-TUBB3 antibody (ABIN153066) Images
Specificity The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
Purification Ammonium sulfate precipitation
Immunogen Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Clone TU-20
Isotype IgG1

Target Details TUBB3

Product Details anti-TUBB3 Antibody Application Details Handling ProductDetails: References for anti-TUBB3 antibody (ABIN153066) Images back to top
Antigen
Alternative Name TUBB3 / TUBB4 (TUBB3 Antibody Abstract)
Background Gene Symbol: TUBB3
Gene ID 10381
Pathways Microtubule Dynamics, M Phase

Application Details

Product Details anti-TUBB3 Antibody Target Details TUBB3 Handling ProductDetails: References for anti-TUBB3 antibody (ABIN153066) Images back to top
Application Notes Western Blot 1-2 μg/mL, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 10 μg/mLWestern Blot - Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunohistochemistry-Paraffin sections - Staining technique: Standard ABC technique (DAB+) Pretreatment: 0.1 % pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT, or High temperature citrate buffer antigen retrieval
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details ProductDetails: References for anti-TUBB3 antibody (ABIN153066) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

ProductDetails: References for anti-TUBB3 antibody (ABIN153066)

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling Images back to top
Product cited in:

Martins, Serrano, Müller, da Cruz E Silva, Rebelo: "BRI2 Processing and Its Neuritogenic Role Are Modulated by Protein Phosphatase 1 Complexing." in: Journal of cellular biochemistry, 2017 (Sample species: Human).

Badizadegan, Thomas, Nagy, Ndishabandi, Miller, Alessandrini, Belkind-Gerson, Goldstein: "Presence of intramucosal neuroglial cells in normal and aganglionic human colon." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 307, Issue 10, pp. G1002-12, 2014 (Sample species: Human). Further details: Flow Cytometry

Kong, Wong, Chan, Lo: "Chronic exposure of adult rats to low doses of methylmercury induced a state of metabolic deficit in the somatosensory cortex." in: Journal of proteome research, Vol. 12, Issue 11, pp. 5233-45, 2013 (Sample species: Rat (Rattus)). Further details: Western Blotting

Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012

Yoo, Kim, Nam, Chung, Choi, Yoon, Won, Hwang: "Chronic effects of pyridoxine in the gerbil hippocampal CA1 region after transient forebrain ischemia." in: Neurochemical research, Vol. 37, Issue 5, pp. 1011-8, 2012 Further details: Western Blotting,Immunohistochemistry

Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, 2009

Jirásek, Cipro, Musilová, Kubecová, Mandys: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, 2009

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007

Jirásek, Písaríková, Viklický, Mandys: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, 2007

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004

P?knicová, Kubátová, Sulimenko, Dráberová, Viklický, Hozák, Dráber: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, 2001

Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000

Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998

Images

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling ProductDetails: References for anti-TUBB3 antibody (ABIN153066) back to top
Supplier Images
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 441-448) (ABIN153066) anti-Tubulin, Beta, 3 (TUBB3) (AA 441-448), (C-Term), (N-Term) antibody
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 441-448) (ABIN153066) anti-Tubulin, Beta, 3 (TUBB3) (AA 441-448), (C-Term), (N-Term) antibody (Image 2)
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 441-448) (ABIN153066) Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (TU-20) [ABIN153066...
Flow Cytometry (FACS) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 441-448) (ABIN153066) Flow Cytometry: beta-III Tubulin Antibody (TU-20) [ABIN153066] - Analysis of PerCP co...