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anti-Rat (Rattus) TUBB3 antibody for Immunofluorescence

Recommended TUBB3 Antibody (supplied by: Log in to see )

Antigen
Tubulin, Beta, 3 (TUBB3) Antibodies
  • 3200002H15Rik
  • AI325297
  • beta-4
  • CDCBM
  • CFEOM3A
  • M(beta)3
  • M(beta)4
  • M(beta)6
  • Tubb
  • tubb3
  • TUBB3
  • Tubb4
  • TUBB4
Epitope
C-Term
34
27
11
7
5
5
5
4
4
4
3
3
2
2
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
189
55
52
11
4
4
4
2
2
2
1
1
Host
Rabbit
101
90
14
12
1
Clonality
Monoclonal
Conjugate
This TUBB3 antibody is un-conjugated
23
12
11
3
3
3
3
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
178
80
80
44
42
36
33
16
13
9
6
2
2
2
Supplier
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Available images

Catalog No. ABIN258508
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.2973765 ABIN955393 IF IHC (p) WB Chicken Ig Log in to see Polyclonal
0.2973765 ABIN151159 ICC IF IHC IHC (wm) WB Chicken Log in to see Polyclonal
0.0010851378 ABIN781811 IF IHC (p) WB Mouse IgG2a AA 436-450 Log in to see n-a
0.0010851378 ABIN258509 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see EP1569Y
0.0010851378 ABIN784021 IF IHC (p) WB Chicken Ig Log in to see Polyclonal
0.0010851378 ABIN784019 EIA IF IHC (p) WB Chicken Ig Log in to see Polyclonal
0.0010851378 ABIN2477006 IF FITC Mouse IgG1 Log in to see TU-20 4
0.0010851378 ABIN2477005 IHC (fro) IF IHC (p) WB Mouse IgG1 AA 441-448 Log in to see TU-20 4
0.0010851378 ABIN1854950 ICC IF IHC WB Mouse IgG2a N-Term Log in to see
0.0010851378 ABIN2380102 IC IF WB Mouse IgG2a Log in to see TuJ-1
0.0010851378 ABIN3059731 ICC FACS IF IP IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-TUBB3 antibody for Immunofluorescence

Similar anti-TUBB3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 8 Antibodies 10 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 8 Antibodies 12 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 49 Antibodies

General

Antigen Tubulin, Beta, 3 (TUBB3) Antibodies
Epitope C-Term
(34), (27), (11), (7), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(189), (55), (52), (11), (4), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(101), (90), (14), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TUBB3 antibody is un-conjugated
(23), (12), (11), (3), (3), (3), (3), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(178), (80), (80), (44), (42), (36), (33), (16), (13), (9), (6), (2), (2), (2)
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Product Details anti-TUBB3 Antibody

Target Details TUBB3 Application Details Handling Images
Cross-Reactivity (Details) Mouse cross reactivity tested by western blot,Immunohistochemistry. rat,cross reactivity tested by Western Blot only.
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues near the C-term of human Beta-Tubulin was used as immunogen.
Clone EP1331Y
Isotype IgG

Target Details TUBB3

Product Details anti-TUBB3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TUBB3 / TUBB4 (TUBB3 Antibody Abstract)
Background Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosisand consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulinbinds to alpha tubulin to form tubulin heterodimer which is post-translationally modified(1). The tubulin dimer complex binds to GTP and assembles onto the positive ends ofmicrotubules. After incorporation into the microtubules, bound GTP is hydrolyzed bybeta tubulin. The stability of the dimer in the microtubules is depended on presence ofbeta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubuleincorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drugresistance in lung cancers (3). Alternate Names: anti-M(beta)3 antibody, anti-M(beta)6 antibody, anti-MC1R antibody, anti-Tubb 3antibody, anti-TUBB 4 antibody, anti-Tubb3 antibody, anti-TUBB4 antibody, anti-Tubulinbeta 3 antibody, anti-Tubulin beta 3 chain antibody, anti-Tubulin beta 4 antibody,anti-Tubulin beta III antibody, anti-beta III Tubulin antibody.
Gene Symbol: TUBB
Gene ID 203068, 22152, 246118
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB3 Antibody Target Details TUBB3 Handling Images back to top
Application Notes Recommended dilutions: Flow Cytometry 1:10, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250-500, Immunoprecipitation 1:50, Western Blot 1:20000
Protocol Protocol specific for Tubulin Beta 3 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (C-Term) (ABIN258508) Western blot: Beta-Tubulin Rabbit Antibody [ABIN258508]- analysis on HeLa cell lysat...
Immunohistochemistry (IHC) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (C-Term) (ABIN258508) Immunohistochemistry: Beta-Tubulin Rabbit Antibody [ABIN258508]- analysis of paraffi...
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (C-Term) (ABIN258508) Imunofluorescence: Beta-Tubulin Rabbit Antibody [ABIN258508]- staining of HeLa cells...