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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||8 Antibodies||10 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Flow Cytometry (FACS)||8 Antibodies||12 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||13 Antibodies|
|Immunofluorescence (IF)||12 Antibodies|
|Immunohistochemistry (IHC)||20 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||16 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Western Blotting (WB)||49 Antibodies|
|Antigen||Tubulin, Beta, 3 (TUBB3) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This TUBB3 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-TUBB3 AntibodyTarget Details TUBB3 Application Details Handling Images
|Cross-Reactivity (Details)||Mouse cross reactivity tested by western blot,Immunohistochemistry. rat,cross reactivity tested by Western Blot only.|
|Immunogen||A synthetic peptide corresponding to residues near the C-term of human Beta-Tubulin was used as immunogen.|
Target Details TUBB3Product Details anti-TUBB3 Antibody Application Details Handling Images back to top
|Alternative Name||TUBB3 / TUBB4 (TUBB3 Antibody Abstract)|
Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosisand consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulinbinds to alpha tubulin to form tubulin heterodimer which is post-translationally modified(1). The tubulin dimer complex binds to GTP and assembles onto the positive ends ofmicrotubules. After incorporation into the microtubules, bound GTP is hydrolyzed bybeta tubulin. The stability of the dimer in the microtubules is depended on presence ofbeta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubuleincorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drugresistance in lung cancers (3). Alternate Names: anti-M(beta)3 antibody, anti-M(beta)6 antibody, anti-MC1R antibody, anti-Tubb 3antibody, anti-TUBB 4 antibody, anti-Tubb3 antibody, anti-TUBB4 antibody, anti-Tubulinbeta 3 antibody, anti-Tubulin beta 3 chain antibody, anti-Tubulin beta 4 antibody,anti-Tubulin beta III antibody, anti-beta III Tubulin antibody.
Gene Symbol: TUBB
|Gene ID||203068, 22152, 246118|
Application DetailsProduct Details anti-TUBB3 Antibody Target Details TUBB3 Handling Images back to top
|Application Notes||Recommended dilutions: Flow Cytometry 1:10, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250-500, Immunoprecipitation 1:50, Western Blot 1:20000|
Protocol specific for Tubulin Beta 3 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling back to top