anti-Human CAMK2G antibody for Immunohistochemistry

Recommended CAMK2G Antibody (supplied by: Log in to see )

Antigen
Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) Antibodies
  • cam2
  • camk2
  • camk2g
  • camkII
  • camkb
  • CaMKII
  • R74975
  • mKIAA0968
  • CAMK
  • CAMK-II
  • CAMKG
  • 5930429P18Rik
  • Camkg
  • calcium/calmodulin-dependent protein kinase II gamma
  • calcium/calmodulin-dependent protein kinase (CaM kinase) II beta
  • calcium/calmodulin-dependent protein kinase II alpha
  • CAMK2G
  • camk2b
  • Camk2a
  • Camk2g
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
107
75
73
20
3
3
3
2
2
2
1
1
1
1
Host
Mouse
70
47
Clonality
Monoclonal
Conjugate
This CAMK2G antibody is conjugated to PE-Atto 594
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
112
64
62
52
40
39
17
7
3
3
1
1
1
1
1
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Catalog No. ABIN2484562
$ 420.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.868737 ABIN2484913 ICC IF IP IHC ELISA WB Atto 633 Mouse IgG1 Log in to see 22B1 2
8.868737 ABIN2484549 ICC IF IP RIA IHC ELISA WB Atto 390 Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484550 ICC IF IP RIA IHC ELISA WB Atto 488 Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484551 ICC IF IP RIA IHC ELISA WB Atto 565 Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484553 ICC IF IP RIA IHC ELISA WB Atto 633 Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484556 ICC IF IP RIA IHC ELISA WB Atto 700 Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484555 ICC IF IP RIA IHC ELISA WB Atto 680 Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484557 ICC IF IP RIA IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484559 ICC IF IP RIA IHC ELISA WB Biotin Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484560 ICC IF IP RIA IHC ELISA WB FITC Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484563 ICC IF IP RIA IHC ELISA WB PerCP Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484561 ICC IF IP RIA IHC ELISA WB HRP Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484564 ICC IF IP RIA IHC ELISA WB PE Mouse IgG1 Log in to see 6G9 2
8.868737 ABIN2484915 ICC IF IP IHC ELISA WB Atto 680 Mouse IgG1 Log in to see 22B1 2
8.868737 ABIN2484914 ICC IF IP IHC ELISA WB Atto 655 Mouse IgG1 Log in to see 22B1 2
8.868737 ABIN2484916 ICC IF IP IHC ELISA WB Atto 700 Mouse IgG1 Log in to see 22B1 2
8.868737 ABIN2484920 ICC IF IP IHC ELISA WB FITC Mouse IgG1 Log in to see 22B1 2
8.868737 ABIN2484919 ICC IF IP IHC ELISA WB Biotin Mouse IgG1 Log in to see 22B1 2
8.868737 ABIN2484921 ICC IF IP IHC ELISA WB HRP Mouse IgG1 Log in to see 22B1 2
8.868737 ABIN2484922 ICC IF IP IHC ELISA WB PE-Atto 594 Mouse IgG1 Log in to see 22B1 2

General

Antigen Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(107), (75), (73), (20), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(70), (47)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CAMK2G antibody is conjugated to PE-Atto 594
(3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(112), (64), (62), (52), (40), (39), (17), (7), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CAMK2G Antibody

Target Details CAMK2G Application Details Handling References for anti-CAMK2G antibody (ABIN2484562) Images
Specificity Detects ~50-60 kDa. Recognizes both phosphorylated and non-phosphorylated forms.
Purification Protein G Purified
Immunogen Partially purified rat CaMKII
Clone 6G9
Isotype IgG1

Target Details CAMK2G

Product Details anti-CAMK2G Antibody Application Details Handling References for anti-CAMK2G antibody (ABIN2484562) Images back to top
Antigen
Alternative Name CaMKII (CAMK2G Antibody Abstract)
Background CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
Gene ID 12322
NCBI Accession NP_033922
UniProt P11798
Research Area Cancer, Hypertrophy, Kinases/Phosphatases
Pathways WNT Signaling, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-CAMK2G Antibody Target Details CAMK2G Handling References for anti-CAMK2G antibody (ABIN2484562) Images back to top
Application Notes
  • WB (1:10000)
  • IHC (1:2000)
  • ICC/IF (1:50)
  • optimal dilutions for assays should be determined by the user.
Comment

0.1 μg/ml was sufficient for detection of CamKII in 20 μg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Restrictions For Research Use only

Handling

Product Details anti-CAMK2G Antibody Target Details CAMK2G Application Details References for anti-CAMK2G antibody (ABIN2484562) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-CAMK2G antibody (ABIN2484562)

Product Details anti-CAMK2G Antibody Target Details CAMK2G Application Details Handling Images back to top
Background publications

Shen, Meyer: "Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 162-6, 1999

Tombes, Krystal: "Identification of novel human tumor cell-specific CaMK-II variants." in: Biochimica et biophysica acta, Vol. 1355, Issue 3, pp. 281-92, 1997

Images

Product Details anti-CAMK2G Antibody Target Details CAMK2G Application Details Handling References for anti-CAMK2G antibody (ABIN2484562) back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (PE-Atto 594) (ABIN2484562) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal An...
Western Blotting (WB) image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (PE-Atto 594) (ABIN2484562) Western Blot analysis of Rat brain membrane lysate showing detection of CaMKII protei...
Immunohistochemistry (IHC) image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (PE-Atto 594) (ABIN2484562) Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 ...
Immunohistochemistry (IHC) image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (PE-Atto 594) (ABIN2484562) Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 ...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (PE-Atto 594) (ABIN2484562) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal An...