Two additional antibodies for their utilization in western blotting are successfuly validated. Specifically, one antibody specific for the detection of PI3K p85 /p55 (pTyr467) has been validated in the human cell line HeLa, while no band was visible in the insect cell line C6/36. Additionally Breast Cancer 2, Early Onset (BRCA2) could be detected in the human cell line MCF7. Human CAPAN-1 cells were used in this assay as negative control.
Three additional antibodies for the detection of different cadherin types have been successfully validated for their utilization in flow cytometry. Specifically, two antibodies specific for the detection of E-Cadherin (Cadherin-1) have been validated in human cell line MCF-7 (ABIN687682 and ABIN1387847) while a K-Cadherin (Cadherin-6) antibody was tested using cell lines MCF-7 and 786-O (ABIN715286).
The ELISA kit for the detection of Matrix Metallopeptidase 9 (MMP9) has now been successfully validated for western blotting. A strong signal was observed in human serum while no MMP9 was detectable in chicken serum.