Recombinant proteins are a core component in many proteomics experiments. In the simplest experiments researchers use recombinant protein products as concentration standards or positive controls for in vitro assays like ELISA or Western blotting. More complex experiments may make use of recombinant proteins with confirmed biological activity. An active recombinant may be introduced in vivo to stimulate a desired outcome or measure the effect of a particular protein on cellular physiology. For example, recombinant CD-40L/CD-154 can be introduced to PBMCs in culture in order to simulate infection induced macrophage activation and stimulate CD-40 dependent production of IL-8.

The purity and consistency of a recombinant protein plays a major role in any technique, but it is particularly important when utilizing this sort of in vivo methodology. Animal derived growth factors or cytokines may be present in recombinant protein preparations because animal products (like BSA) are frequently used in traditional recombinant protein manufacturing processes. These contaminants may not be detectable through traditional methods for assessing purity, but even at trace concentrations they can alter physiological response and skew results in an unpredictable manner.

antibodies-online is pleased to announce our recent partnership with Colorado Springs based assay and reagent manufacturer HemoGenix® . HemoGenix® assays offer an unprecedented level of flexibility and function to researchers that need to assay stem-cell and primitive-cell development. The HemoGenix® product line at antibodies-online includes several unique products for assaying viability, proliferation, differentiation, and metabolism in various cell populations.

HemoGenix® CAMEO-96 CFU kits are the only commercially available assays that permit simultaneous comparison of both cell proliferation and differentiation. HemoFLUOR-96 assays replace traditional time-consuming colony-counting CFC techniques, utilizing a fluorescence based detection method for determination of cell viability and proliferation. The HemoFLUOR-96 assay can also be multiplexed with FACS analysis or bioluminescent assay techniques to provide a more robust analysis of hematopoietic stem cell populations.

Virology is a topic within the greater domain of Microbiology focused on the study of viruses and virus like particles. It focuses in particular on viral structure and evolution, the virus “life cycle” and ultimately the interaction with their host cells. Knowledge of all these aspects is crucial to prevent, diagnose, treat, and monitor virus-borne diseases.

antibodies-online offers a range of antibodies for virology studies. Among others, epitope-mapped mouse monoclonal antibodies as well as partially humanized chimeric recombinant antibodies against viruses Human Immunodeficiency Virus 1 , Hepatitis C Virus subtype 1bt, and Bovine Papillomavirus type 1 antigens.

Determining whether two proteins interact is a characteristically challenging endeavor, particularly in systems where low concentration makes traditional co-immunoprecipitation experiments nontrivial.

The proximity ligation assay (PLA) allows researchers to rapidly and simply determine whether two proteins interact.

The proximity ligation assay (PLA) is the result of a harmonious marriage between affinity proteomics and molecular biology techniques. The PLA employs a paired set of antibodies that are conjugated to special matching oligonucleotide probes. Simple, reliable rolling circle DNA amplification is leveraged to provide a readout that confirms protein interaction.

antibodies-online currently offers more than 1,500 reagents (747 individual antibodies and 766 verified antibody-pairs) for use in proximity ligation assay experiments.

Read more about the PLA.

antibodies-online is pleased to announce that we have recently partnered with AbSci, a premium manufacturer of life-science reagents. Based in Nanjing, China, AbSci offers thousands of high-quality, purified recombinant proteins. More than 3,100 AbSci recombinant proteins expressed in E.coli, Yeast, mammalian cell, and baculovirus expression systems are now available at antibodies-online. Check out all of the new AbSci products at antibodies-online.