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Independent Validation

47 products are now successfully validated

Independent Validation

The antibody for the detection of Prostate Specific Antigen (PSA) has now been successfully validated for flow cytometry. A strong signal was observed in the androgen-sensitive human prostate adenocarcinoma cellline LNCaP. The peak is clearly separated from the isotype stained probe.

Prostate Specific Antigen (PSA) is a typical serine protease with Semenogelin-1 as its substrate. The cleavage of Semenogelin-1 liquefies the seiminal fluid. PSA is a tissue-specific biomarker and not a general marker for prostate cancer. PSA is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. Increased levels of PSA can be indicative of prostate cancer.
Independent Validation
In mid 2013 the "Independent Validation Initiative" started as a partnership between Science Exchange and antibodies-online.com. The project aims to enable scientists to select products (presently antibodies or ELISA kits) of their interest to be validated by labs from top academic research institutes.

Since we started the initiative we have successfully validated 47 products. The first was a anti-Fibrinogen antibody, followed by a couple of other interesting antibodies and ELISA kits. The latest succesfully validated product is an antibody for the detection of Prostate specific Antigen (PSA). A few more products are currentlly being validated.

If you are interested in having a product validated, you can simply click the "Request validation" button on the product site. When requesting a validation you can specify the application the product should be validated for. Validation is free for all customers and costs will be covered by the respective supplier. Here is a full list of successfully validated products:
Catalog No. Antigen Name Product Type Tested for
ABIN685477 Allograft Inflammatory Factor 1 (AIF1) Rabbit IgG IF
ABIN197433 Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) Rabbit IgG IF
ABIN707576 Aquaporin 2 (Collecting Duct) (AQP2) Rabbit IgG IHC
ABIN729343 Cadherin 5 (CDH5) Rabbit IgG IF
ABIN1690718 Carbohydrate Antigen 15-3 (CA15-3) Mouse IgG1 Flow Cytometry
ABIN968006 Clathrin (AA 4-171,Heavy Chain) Mouse IgG1 IHC
ABIN708086 Coagulation Factor III (thromboplastin, Tissue Factor) (F3) Rabbit IgG IHC
ABIN1112984 DYKDDDDK Tag Mouse IgG1 IF
ABIN734003 Elastin (ELN) (C-Term) Rabbit IgG IHC
ABIN673854 Fibrinogen Rabbit IgG ELISA
ABIN750991 Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) Rabbit IgG WB
ABIN728713 NK2 Homeobox 1 (NKX2-1) (N-Term) Rabbit IgG WB
ABIN1543584 Prostate Specific Antigen (PSA) Rabbit IgG IHC
ABIN675024 Tight Junction Protein 1 (Zona Occludens 1) (TJP1) Rabbit IgG IHC
ABIN705056 Tumor necrosis factor ligand superfamily member 9 (TNFSF9) Rabbit IgG WB
ABIN967416 Tumor Protein P53 (TP53) Rabbit IgG WB
ABIN364986 Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ) ELISA Kit ELISA
ABIN364989 alpha-Fetoprotein (AFP) Rabbit IgG WB
ABIN365222 Angiogenin, Ribonuclease, RNase A Family, 5 (ANG) ELISA Kit ELISA
ABIN810667 Angiopoietin 1 (ANGPT1) ELISA Kit ELISA
ABIN578045 Angiopoietin-Like 2 (ANGPTL2) (Human) ELISA Kit ELISA
ABIN366603 CD105 (Endoglin / TGF-Beta1/3 Receptor) ELISA Kit ELISA
ABIN1136417 Chromosome 19 Open Reading Frame 80 (C19ORF80) ELISA Kit ELISA
ABIN1000178 cortisol (COR) ELISA Kit ELISA
ABIN365082 Cystatin C (CST3) ELISA Kit ELISA
ABIN419782 Defensin, alpha 3, Neutrophil-Specific (DEFa3) ELISA Kit ELISA
ABIN1372755 Dehydroepiandrosterone Sulfate (Human) ELISA Kit ELISA
ABIN1372754 Dehydroepiandrosterone Sulfate (Rat) ELISA Kit ELISA
ABIN837930 Epidermal Growth Factor Receptor (Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog, Avian) (EGFR) ELISA Kit ELISA
ABIN1114880 Ferritin (FE) (Human) ELISA Kit ELISA
ABIN512884 Follicle-Stimulating Hormone (FSH) ELISA Kit ELISA
ABIN816582 Interferon Regulatory Factor 4 (IRF4) (Mouse) ELISA Kit ELISA
ABIN365163 IL-6 (Human) ELISA Kit ELISA
ABIN414887 Interleukin 8 (IL8) ELISA Kit ELISA
ABIN365215 Interleukin 18 (IL18) ELISA Kit ELISA
ABIN365165 Leptin (LEP) ELISA Kit ELISA
ABIN365998 Lipocalin 2 (LCN2) ELISA Kit ELISA
ABIN512262 Luteinizing hormone (LH) ELISA Kit ELISA
ABIN818177 Matrix Metalloproteinase 9 (MMP9) ELISA Kit ELISA
ABIN1690717 NGAL/MMP-9 complex ELISA Kit ELISA
ABIN368630 Pro-Brain Natriuretic Peptide (NT-ProBNP ELISA Kit ELISA
ABIN457074 Soluble Cluster of Differentiation 14 (sCD14) ELISA Kit ELISA
ABIN366828 Thrombospondin 1 (THBS1) ELISA Kit ELISA
ABIN510312 Thyroid Stimulating Hormone (TSH) (Human) ELISA Kit ELISA
ABIN365420 TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA Kit ELISA
ABIN367608 Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) ELISA Kit ELISA
ABIN365402 Transforming Growth Factor, beta 1 (TGFB1) ELISA Kit ELISA