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Independent Validation

Antibody to detect Green Fluorescent Protein (GFP) is now successfully validated

Independent Validation

The antibody for the detection of Insulin-Like Growth Factor 1 Receptor (IGF1R) has now been successfully validated for western blotting. A strong signal was observed in MCF-7 cells, while no band was visible in PC3 cells.


Independent Validation
In mid 2013 the "Independent Validation Initiative" started as a partnership between Science Exchange and antibodies-online.com. The project aims to enable scientists to select products (presently antibodies or ELISA kits) of their interest to be validated by labs from top academic research institutes.

Since we started the initiative we have successfully validated 69 products. The first was a anti-Fibrinogen antibody, followed by a couple of other interesting antibodies and ELISA kits. The latest succesfully validated product is an antibody for the detection of Insulin-Like Growth Factor 1 Receptor (IGF1R). A few more products are currentlly being validated.

If you are interested in having a product validated, you can simply click the "Request validation" button on the product site. When requesting a validation you can specify the application the product should be validated for. Validation is free for all customers and costs will be covered by the respective supplier.

Here is a full list of successfully validated products:

Catalog No.Antigen NameProduct TypeTested for
ABIN685477Allograft Inflammatory Factor 1 (AIF1)Rabbit IgGIF
ABIN197433Amyloid beta A4 precursor protein (APP)Rabbit IgGIF
ABIN707576Aquaporin 2 (Collecting Duct) (AQP2)Rabbit IgGIHC
ABIN729343Cadherin 5 (CDH5)Rabbit IgGIF
ABIN1690718Carbohydrate Antigen 15-3 (CA15-3)Mouse IgG1Flow cytometry
ABIN679493Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7)Rabbit IgGIHC
ABIN671376CD4 Molecule (CD4)Rabit IgGIHC
ABIN968006Clathrin (AA 4-171,Heavy Chain)Mouse IgG1IHC
ABIN708086Coagulation Factor III (thromboplastin, Tissue Factor) (F3)Rabbit IgGIHC
ABIN670671Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1)Rabbit IgG1IHC
ABIN1112984DYKDDDDK TagMouse IgG1IF
ABIN734003Elastin (ELN) (C-Term)Rabbit IgGIHC
ABIN1756134Epidermal Growth Factor Receptor (EGFR)Mouse IgG1Flow cytometry
ABIN1386772Eukaryotic Translation Initiation Factor 4E (EIF4E)Rabbit IgGIHC
ABIN673854Fibrinogen (FBG)Rabbit IgGELISA
ABIN398304Green Fluorescent Protein (GFP)Rat IgG2aWB
ABIN726575Insulin-Like Growth Factor 1 Receptor (IGF1R)Rabbit IgGWB
ABIN750991 Low Density Lipoprotein Receptor-Related Protein 2 (LRP2)Rabbit IgGIF
ABIN1760652mCherryMouse IgGWB
ABIN736821Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2)Rabbit IgGIF
ABIN678668Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182)Rabbit IgGIHC
ABIN686482Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)Rabbit IgGIHC
ABIN754183Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5)Rabbit IgGIHC
ABIN728713NK2 Homeobox 1 (NKX2-1) (N-Term)Rabbit IgGWB
ABIN687337Occludin (OCLN)Rabbit IgGIF
ABIN1543584 Prostate Specific Antigen (PSA)Mouse IgG1Flow Cytometry
ABIN1714069Runt-Related Transcription Factor 3 (RUNX3)Rabbit IgGIF
ABIN675024Tight Junction Protein 1 (Zona Occludens 1) (TJP1)Rabbit IgGIHC
ABIN677318Tumor Necrosis Factor (TNF)Rabbit IgGIF
ABIN705056Tumor necrosis factor ligand superfamily member 9 (TNFSF9)Rabbit IgGIHC
ABIN967416Tumor Protein P53 (TP53) (full length)Rabbit IgGWB
ABIN733208V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1)Rabbit IgGIHC
ABIN762896Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (N-Term)Rabbit IgGIHC
ABIN675728Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (N-Term)Rabbit IgGIHC
ABIN364989alpha-Fetoprotein (AFP)ELISA KitELISA
ABIN364986Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ)ELISA KitELISA
ABIN365222 Angiogenin, Ribonuclease, RNase A Family, 5 (ANG)ELISA KitELISA
ABIN810667Angiopoietin 1 (ANGPT1)ELISA KitELISA
ABIN578045Angiopoietin-Like 2 (ANGPTL2)ELISA KitELISA
ABIN367349C-Telopeptide of Type I CollagenELISA KitELISA
ABIN366603CD105 (Endoglin / TGF-Beta1/3 Receptor)ELISA KitELISA
ABIN1136417Chromosome 19 Open Reading Frame 80 (C19ORF80)ELISA KitELISA
ABIN1721161CotinineELISA KitELISA
ABIN365082Cystatin C (CST3)ELISA KitELISA
ABIN367853Cystatin C (CST3)ELISA KitELISA
ABIN1114508Cystatin C (CST3)ELISA KitELISA
ABIN419782Defensin alpha 3 (DEFa3)ELISA KitELISA
ABIN837930Epidermal Growth Factor Receptor (Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog, Avian) (EGFR)ELISA KitELISA
ABIN1114880Ferritin (FE)ELISA KitELISA
ABIN512884Follicle Stimulating Hormone (FSH)ELISA KitELISA
ABIN365163IL-6ELISA KitELISA
ABIN1563240Immunoglobulin YELISA KitELISA
ABIN816582Interferon Regulatory Factor 4 (IRF4)ELISA KitELISA
ABIN365215Interleukin 18 (IL18)ELISA KitELISA
ABIN414887Interleukin 8 (IL-8)ELISA KitELISA
ABIN365165Leptin (LEP)ELISA KitELISA
ABIN365998Lipocalin 2 (LCN2)ELISA KitELISA
ABIN512262Luteinizing hormone (LH)ELISA KitELISA
ABIN818177Matrix Metalloproteinase 9 (MMP9)ELISA KitELISA
ABIN1690717NGAL/MMP-9 complexELISA KitELISA
ABIN368630Pro-Brain Natriuretic Peptide (NT-ProBNPELISA KitELISA
ABIN457074Soluble Cluster of Differentiation 14 (sCD14)ELISA KitELISA
ABIN511617TestosteroneELISA KitELISA
ABIN366828Thrombospondin 1 (THBS1)ELISA KitELISA
ABIN510312Thyroid Stimulating Hormone (TSH)ELISA KitELISA
ABIN365420TIMP Metallopeptidase Inhibitor 1 (TIMP1)ELISA KitELISA
ABIN367608Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI)ELISA KitELISA
ABIN365402Transforming Growth Factor, beta 1 (TGFB1)ELISA KitELISA
ABIN367720Vascular Cell Adhesion Molecule 1 (VCAM1)ELISA KitELISA