Here you can find a new user test for CD3 PE, CD4 FITC and CD14 FITC antibodies in flow cytometry (FACS). If you are interested in testing one of our antibodies yourself contact us: »Contact

Staining protocol:

1. 25µl of cell + 5µl of each antibodies
2. Incubate in dark environment at RT for 20 minutes
3. Add 2ml of lysing solution
4. Spin at 1250 RPM for 5 minutes
5. Resuspend cells in 2 ml PBS + 0,2%BSA
6. Spin at 1250 RPM for 5 minutes
7. Resuspend cells in 0,5 ml PBS + 0,2%BSA
8. Analyze on flow cytometer

Lysing solution:

NH4Cl: 8,3g

NaHCO3: 1g

EDTA: 0,038g

H2O: 1000ml

Results:

CD3 PE (ABIN94062) and CD14 FITC (ABIN93966) antibodies

CD3 PE (ABIN94062) and CD4 FITC antibodies

Here you can find the tested antibodies:

CD3 PE antibody
CD4 FITC antibody
CD14 FITC antibody

Take a look at all CD-antigen antibodies here: Show antibodies