Leukocyte trafficking is strongly impaired in a mouse model for Leukocyte adhesion deficiency II but lymphocyte homing in the spleen is still normal, as Sviatlana Yakubenia from the Max Planck Institute for Molecular Biomedicine in Munster, Germany discovered together with colleagues from other German science institutions. Note: The scientists used this anti-KLH antibody among other antibodies for their research: anti-KLH antibody (ABIN129511).

Leukocyte adhesion deficiency II (LAD II) is a rare hereditary disease, characterised by the lack of fucosylated structures like selectin ligands. Fucose is a monosaccharide, responsible for cell-to-cell communication. In LAD II, a defective GDP-fucose transporter leads to developmental problems and immune deficiencies which are a result of the lacking fucosylated selektin ligands.

The scientists investigated LAD II in Slc35c1 -/- mice. E-, L-, and P-selectin dependent leukocyte rolling, which precedes leukocyte migration through the endothelium to the site of inflammation, did not take place in those mice. The leukocytes also did hardly stick to the endothelium.

The literature describes the T-cell dependent antibody reaction against bacteriophage PhiX174 and Keyhole Limpet Haemocyanin (KLH) as being normal in LAD II which suggests that T-cell and B-cell function is not impaired.KLH is a copper containing oxygen transporter serving the same purpose in many molluscs and arthropods that haemoglobin does in vertebrates.

The scientists discovered that lymphocyte differentiation is reduced to 1-2% in the lymph nodes but is still normal in the spleen. Slc35c1 -/- mice thus show strong deficiencies in leukocyte migration but normal antigen presentation in the spleen which may explain the normal lymphocyte function observed in LAD II.

Anti-KLH-antibody used in this publication:

anti-KLH antibody (ABIN129511)

Related antibodies on antibodies-online.com:

Slc35c1

KLH

Leukocyte antigen CD37

Leukocyte antigen CD84

Leukocyte antigen CD97

Fucose

E-Selektin

L-Selectin

P-Selectin

P-Selectin Ligand

T-cells

B-cells

Lymphocytes

Haemoglobin

Endothelium

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