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Frequently used: phospho-AKT1 monoclonal antibody

Area: Diabetes, Apoptosis, Cancer
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There are three members in the AKT protein family in humans: AKT1, AKT2, and AKT3. These enzymes are members of the serine/threonine-specific protein kinase family. AKT1 (or AKT without numeral) regulates many downstream effectors. AKT1 is able to induce protein synthesis pathways, and is therefore a key signalling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth.
AKT1 can block apoptosis, and thereby promote cell survival. Hence, AKT1 has been implicated as a major factor in many types of cancer due its involvement in angiogenesis and tumor development.

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein. This product was purified from concentrated tissue culture supernate by Protein A chromatography. BLAST analysis was used to suggest cross-reactivity with AKT1 pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. AKT1 pS473 shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Source:
Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses. J. Cell Science 114:2903- 2910. Alessi, D. R. (2001).

Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14. Jones,P.F., Jakubowicz,T., Pitossi,F.J., Maurer,F. and Hemmings,B.A. (1991)

Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc. Natl. Acad. Sci. U.S.A. 88 (10), 4171-4175. Staal,S.P. (1987)

Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma. Proc. Natl. Acad. Sci. U.S.A. 84 (14), 5034-5037.
02.03.2010 | David Kitz Kramer   RSS Feed   New Antibodies   Bookmark and Share

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