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New: Antibodies and Research Tools for Allergy Research

Immunoglobulin E (IgE), and the interaction with it’s receptors expressed on mast cells and basophils, are main players in the pathomechanism of IgE mediated allergic reactions (allergic rhinitis, asthma, atopic dermatitis, food allergies). Purified human IgE protein, recombinant IgE receptors, and biochemically and functionally well characterized monoclonal antibodies, directed against different epitopes on these players, are tools of central importance for allergy research and diagnostics, and also for the identification and development of novel anti-allergic drugs.
Currently available:

Human IgE protein (SUS-11), a highly affinity purified human IgE derived from B cells of healthy donors (no myeloma origin). It has been and is used in numerous research- and development laboratories for various in vitro and in vivo applications.

Monoclonal anti-human IgE antibody (BSW17) recognizes an epitope spanning across the C3 and C4 domains of human IgE and inhibits the binding of IgE to its high affinity receptor. Therefore, apart from classical applications (ELISA, FACS, Western Blot), BSW17 is the antibody of choice to study the mechanism of IgE dependent activation of mast cells and basophils. The properties of this monoclonal anti-human IgE Antibody have also been described in a series of international publications (see below for a selection).

Monoclonal anti-human IgE antibody (Le27) is optimally suitable for in vitro tests like ELISA, FACS, W-blot. In combination with human IgE anbtobody SUS-11 this monoclonal antibody is optimally suited for controlled mast cell activation.

A set consisting of all three components offers an ideal basis for the development of customized screening assays and ELISA for the determination of IgE concentrations in human blood samples.

These products are also available as functionally active conjugates with biotin and other standard labels (e.g. AP, FITC, PE) and can also be ordered in azide free buffer for functional tests using living cells. They are delivered with a certificate of analysis documenting purity and target binding. In case of proven quality deficiencies we guarantee take back and money refunding.

Source: Vogel, Keller-Gautschi, Baumann et al.:”Designed Ankyrin Repeat proteins as Anti-Idiotypic-Binding Molecules.” In: Annals of the New York Academy of Sciences, Vol. 1109 Issue Autoimmunity, Part A Basic principles and New Diagnostic Tools, pp9-18, 2007

Hantusch, Krieger, Untersmayr et al.:”Mapping of conformatorial IgE epitopes on PHI p 5a by using mimotopes from a phage display library.” In: Journal of Allergy and Clinical Immunology, Vol.114,Issue 6, pp.1294-1300 ,2004

Millauer, Zuercher, Miescher et al.:”High igE in rheumatoid athritis (RA) patients is complexed with anti-IgE autoantibodies”.In: Clin Exp Immunol. 115(1),pp 183-188, 1999

Rudolf, Vogel, Kricek et al.:” Epitope-Specific Antibody Response to IgE by Mimotope Immunization.” In: The Journal of Immunolgogie,Vol160, pp 3315-3321, 1998

Pachlopnik, Stämpfli, Rudolf et al.:”Antigen Interaction and Heat Inactivation Expose New Epitopes on Human IgE”. In: Int. Arch Allergy Immunol , Vol 117, pp231-238, 1998