Detection of green fluorescent protein (GFP) from Aequorea victoria is a common tool in various applications ranging from expression studies to production of recombinant protein. This rat monoclonal anti-GFP antibody (clone 3H9) is suitable for immunoblotting and ELISA. This versatile anti-GFP and fusions thereof are attractive tools for biochemical applications like identification of interacting proteins or Western Blotting.