The human gene HLA-G (Human Leukocyte Antigen G), or, HLA-G histocompatibility antigen, class I, G, is a heterodimer consisting of a heavy chain and a light chain. HLA-G is similar to MHC Class I molecules which are one of two primary classes of major histocompatibility complex proteins.

Also MHC Class I molecules consist of two polypeptide chains, the alpha- and beta2-microglobulin (b2m) polypeptides. Histocompatibility proteins play a role in immunological funtions. MHC help recognize and trigger destrucion of infected cells and HLA-G are thought to play a role in immune tolerance during pregnancy.

Below is a list of antibodies suited for detection of HLA-G and related proteins:

Clone	ABIN	FACS	IHC(f)	IHC(p)	WB	ELISA	Blocking	Specificity
MEM-G/9	94368	excellent	excellent	negative	negative	excellent	negative	HLA-G1, G5 (conformational)
MEM-G/11	94375	excellent	excellent	not tested	negative	good	negative	HLA-G1 (conformational)
87G	94362	excellent	excellent	negative	negative	very good	excellent	HLA-G1, G5 (conformational)
01G	94359	very good	very good	negative	negative	good	negative	HLA-G1(conformational)
MEM-G/1	94366	poor	very good	very good	excellent	see 1)	negative	All HLA-G isoforms (denat.)
MEM-G/2	94384	poor	very good	excellent	very good	not tested	negative	All HLA-G isoforms (denat.)
MEM-G/4	94378	poor	not tested	not tested	excellent	not tested	negative	HLA-G1, G2, G5 (denat.)
5A6G7	94379	poor	excellent	excellent	very good	excellent	negative	HLA-G5, G6 (anti peptide)
2A12	94382	excellent	very good	excellent	excellent	good	negative	HLA-G5, G6 (anti peptide)
Related antibodies
MEM-123	94336	excellent	not tested	not tested	very good	very good	not tested	MHC-I, and HLA-G1, G5
W6/32	94332	excellent	excellent	negative	very good	excellent	not tested	MHC-I (conform.), standard
B2M-01	93902	excellent	not tested	not tested	excellent	excellent	not tested	beta2-microglobulin

1) Antibody MEM-G/1 is an excellent detection antibody for ELISA detection of free HLA-G heavy chains.

Abbreviations: denat. - denatured, conform. - conformational, FACS - Flow cytometry, IHC - Immunohistochemistry, (f) - Frozen sections, (p) - Paraffin sections, WB - Western Blotting