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GPCR
G protein-coupled receptors (GPCRs) are the largest family of transmembrane receptors and are responsible for the transduction of a diverse range of extracellular signals. The range of physiological processes mediated by GPCRs makes them one of the most important classes of proteins for drug discovery.  » 
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There are now 17,455 products available from on the antibodies-online.com platform. seeks to be a socially responsible company that is committed to charitable acts. Their mission is to participate in bioscience progress through supporting the scientific and medical research community by donating charitable organisations, f. e. via a cooperation with Together we can.

We offer a variety of peer-validated polyclonal and monoclonal antibodies mainly focused on human targets, a couple of secondary antibodies mainly capable in Western Blot and ELISA, and a few proteins like sepharose coupled Protein A and G.

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Independent Validation
Independent Validation

The antibody for the detection of Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (N-Term) has now been successfully validated for immunohistochemistry. A signal was observed in human glioma tissue, while no signal could be detected in human brain tissue.

The WNT gene family consists of structurally related genes encoding secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis.  » 
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(…) Since last summer Andreas Kessel and Tim Hiddemann from antibodies-online.com support the initiators of Science Exchange. The former spin-off headquartered in Aachen currently delivers more than one million antibodies to laboratories all over the world. It is the task of this cooperation to make antibodies to provide qualitative comparability for antibodies, which are used in biomedical sciences. Scientists and manufacturers can suggest antibodies for validation, which will then be tested in independent laboratories of one of the 1,400 research facilities that build the Science Exchange network. After successful testing the “Independently Validated" label with individual identification number will be granted. This label is intended to be not only a tool for easy validation of scientific studies. Furthermore, with purchase of tested products researchers obtain certainty that antibodies work as it is described in test protocols – and that they are the most suitable choice for the respective question. (...) Source: http://www.spiegel.de/wissenschaft/medizin/qualitaet-der-forschung-rettet-die-wissenschaft-a-959629.html
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Independent Validation
Independent Validation

The antibody for the detection of Runt-Related Transcription Factor 3 (RUNX3) has now been successfully validated for immunofluorescence. A strong signal was observed in the human cellline A431 (epidermoid carcinoma), while no signal could be detected in the isotype staining and staining of HEK293 cells.

A complex formed of a heterodimer of Runt-Related Transcription Factor 3 (RUNX3) and a beta subunit binds to the core DNA sequence 5'-YGYGGT-3' found in a number of enhancers and promoters. That can either activate or suppress transcription. RUNX3 can also interact with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer.  » 
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Independent Validation
Under the title "The Antibody Challenge" Jeffrey M. Perkel wrote in BioTechniques (BioTechniques, March 2014, pp. 111-114) about the issues of reproducibility. In his interesting article he also described the independent validation initiative and interviewed our collegue Stefan Pellenz, who runs the validation initiative at antibodies-online: "Recently, Iorns launched a new effort to help. In collaboration with antibody distributor antibodies-online.com, the ScienceExchange Independent Validation Initiative pairs vendors with independent labs that can provide third-party validation of antibody efficacy for several hundred dollars per test.

The initiative kicked off in July 2013 but has only gained traction in the past few months, says Iorns. “We've done hundreds of tests so far, and we're planning to do 10,000 this year.”

Successfully validated antibodies are awarded a green check mark “badge” for the vendor's web site. “We are trying to establish this as kind of a sign of quality,” says Stefan Pellenz, who runs the validation initiative at antibodies-online. But companies gain more from the process than just a logo, he adds; it is also a marketing tool, conveying to users that their reagent vendors take quality control seriously. Furthermore, the validating labs can work with the vendor (via Science Exchange) for troubleshooting. In one case, an ELISA kit shipped with a bad dilution buffer that was masking the target protein signal. Once the lab figured that out and reported it back to the vendor, the kit was updated subsequently passed validation “with flying colors,” Pellenz says.  » 
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MagnaMedics
antibodies-online.com now provides 98 different magnetic beads from our new supplier .

is specialized in the development of paramagnetic particles to separate cells, proteins or DNA in a fast and sufficient approach.

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Independent Validation
Independent Validation

The antibody for the detection of Prostate Specific Antigen (PSA) has now been successfully validated for flow cytometry. A strong signal was observed in the androgen-sensitive human prostate adenocarcinoma cellline LNCaP. The peak is clearly separated from the isotype stained probe.

Prostate Specific Antigen (PSA) is a typical serine protease with Semenogelin-1 as its substrate. The cleavage of Semenogelin-1 liquefies the seiminal fluid. PSA is a tissue-specific biomarker and not a general marker for prostate cancer. PSA is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. Increased levels of PSA can be indicative of prostate cancer.  » 
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Independent Validation
Independent Validation

The antibody for the detection of Cadherin 5 (CDH5) has now been successfully validated for immunofluorescence. A clear signal of the calcium-depending adhesion molecule was achieved in murine liver tissue while murine brain tissue (known to be negative for cadherin 5) remained unstained.

Cadherin 5 (also called CD144) is a vascular endothelial cadherin. This type of cadherins appears at the contact site of endothelial cells. They are functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, and additional they fundction also as a desmosome.  » 
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Spring Bioscience
There are now more than 2,600 products available from on the antibodies-online.com platform. The strategy of Spring Bioscience is to develop a carefully selected portfolio of products that will provide both the research and diagnostic communities with strategic antibody biomarkers for assessing protein expression in tissue. Spring Bioscience complements these antibody products with an elegant selection of advanced detection and ancillary products that are useful in the field of immunohistochemistry (IHC). Spring Bioscience further developed the production of monoclonal antibodies from rabbit. The most famous rabbit monoclonal antibody is the actual gold standard for detection of the Estrogen Receptor 1 (ESR1) in IHC. Browse through all products.
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Image for Cytokeratin 1-19 (pan-) antibody
  • Their purpose is to function as a barrier, protecting the body from mechanical damage, invading micro organisms and the loss of body fluids.
  • Detection of epithelial cells has a central role in research on cancer diseases.
  • A range of antibodies has been established for detection of individual epithelial cells.
  • Some standard markers are: anti-epithelial membrane antigen (EMA) antibody and pan-cytokeratin antibody
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Supplier: Rockland
Rockland
20 products of the series TrueBlot® from Rockland are now available for North-America, South America and China on antibodies-online.com. Rockland offers a series of products called TrueBlot® to improve your result in IP and Western Blot.  » 
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ReliaTech
There are 438 products of now available on the antibodies-online.com platform.

stands for Receptor Ligand Technologies GmbH. The company is located close to Braunschweig in Germany. ReliaTech develops, produces and sells high quality research reagents for angiogenic and lymphangiogenic research.

You will find high quality primary antibodies, peptides and supplements for cell culture on antibodies-online.com. Some example primary antibodies from ReliaTech can detect a variety of growth and angiogenic cytokines.

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Abbiotec
There are now 2008 products available from on the antibodies-online.com platform. specializes in the development and manufacture of viral antigens and anti-viral antibodies for diagnosis, monitoring, and treatment of significant viral infectious diseases.

You will find a variety of primary antibodies against influenza viruses as well as HI viruses but also a wide range of other viruses, and proteins from the same set of viruses.

Some example primary antibodies from include antibodies that can detect antigens of the actual seasonal flu 2013/2014. Browse through all products.
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Abbiotec
There are now 4,212 products available from on the antibodies-online.com platform.

focus on developing monoclonal and polyclonal antibodies that are unique in binding a new epitope or in recognizing a new target. They offer more than 1,000 primary antibodies. Their products enable the study of human biology and disease at the cellular and molecular levels, with cross applications in diagnostic assays that may eventually lead to promising therapeutics.

You will find primary antibodies, secondary antibodies, isotype controls as well as proteins from Abbiotec. For example Abbiotec offers primary antibodies that can detect a wide choice of human epitopes. The antibodies are applicable in Western Blot, ELISA or Immunohistochemistry.

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Supplier:
EnCor Biotechnology
There are now 66 products available from on the antibodies-online.com platform.

focus on developing primary antibodies of extrem high quality for western blot, ELISA and immunostaining.

You will actually find all available primary antibodies from on this platform. Browse through all products.
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antibodies-online.com offers a wide range of immunology products covering research areas such as apoptosis, cytokines, cytokine receptors, chemokines, CD Markers and adhesion molecules.

Some example products are the following:

The above-mentioned ELISA kits are known for their batch-to-batch consistency as well as efficient delivery time.

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was founded in 2011 and provides high-quality, industry-leading pharmaceutical products. This specializes in high quality extracellular matrix products for various purposes such as cell attachment, cell growth, cell differentiation, cell migration, stem cell research, tissue engineering and tissue morphogenesis.

The product range of is as follows:

  • Human Microvascular Endothelial Cells (Various Tissues)
  • 3-Dimensional Tissue Engineering
  • Coated Plates
  • siRNA
  • RFP-Labeled Cells
  • GFP-Labeled Cells
  • Stables shRNA transfected HUVECs
  • Mouse Brain Microvascular Endothelial Cells
  • Isotopic Control IgG
  • Recombinant Adenovirus
  • Functional Antibody
  • Collagen Hydrogels
  • GFP Expressing Human Fibroblasts
  • Anti-Human Antibody
  • Anti-Mouse Antibody
offers a range of Type I collagen products derived from various sources.

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There are now 140 products available from on the antibodies-online.com platform. provides quality antibodies and professional services such as protein gene cloning, expression, binding confirmation and CHO cellline generation for stable expression.

You will find primary antibodies, secondary antibodies as well as proteins from .

Some example primary antibodies from include antibodies that can detect recombinant proteins containing an epitope tag such as the HIS-tag, the HA-tag, GST, RFP (N-Term) and GFP (N-Term).

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was founded in 2002 and is a biotechnology company commited to the R&D and innovation of immune-based molecules and tools. offer ELISA kits for the quantitative & qualitative determination of TNF, TNF blockers and anti-TNF blockers.

Some example ELISA kits available from are suited to detect Infliximab (trade name Remicade; a monoclonal antibody against tumour necrosis factor alpha), Adalimumab (a TNF inhibitor), Etanercept (a TNF inhibitor), Bevacizumab (trade name Avastin; an angiogenesis inhibitor, a drug that slows the growth of new blood vessels), Trastuzumab (trade names Herclon, Herceptin; a monoclonal antibody that interferes with the HER2/neu receptor), and Rituximab (trade names Rituxan,MabThera; a chimeric monoclonal antibody against CD20).

ELISA kits available on anibodies-online.com are suited for sandwich ELISA assays and suited for samples such as serum, plasma and other biological fluids.

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The range focuses on providing molecular biology reagents, antibiotics, lab plasticware and recombinant proteins, with a particular emphasis on DNA ladders and protein markers.

The DNA ladders and protein markers from are highly stable, available in ready-to-use formats (containing tracking dyes) and provide increased reference points as well as good band resolution, making them ideal tools for molecular biology research.

The DNA markers range includes, for example, the 100bp DNA ladder, the 50bp DNA ladder, the a 1kbp DNA ladder , the Kplus DNA ladder and the XLarge DNA Ladder.

There are also prestained protein ladders such as the BlueRAY Prestained Protein Ladder, the BLUeye Prestained Protein Ladder and the BLUelf Prestained Protein Ladder.

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specializes in supplying biological materials made from animal venom. The company offers venom peptides for basic research and drug discovery solutions based on its venom collection and expertise.

Synthetic peptide toxins (from ) issued from venomous animals are ideal tools for studying ion channels. They also demonstrate significant potential for the development of drugs for a wide variety of pathologies such as pain, auto-immune diseases, cancer or diabetes. possesses the expertise to create highly quality venom peptides.

Currently antibodies-online.com offers 123 products from .

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aims to provide innovative, high-quality assay kits, recombinant proteins, antibodies and other research tools to accelerate drug discovery in disease-related areas such as cancer, diabetes, obesity and Alzheimer's disease.

offers an extensive line of metabolism assay kits that can be used to measure metabolites. Metabolism is a term that is used to describe all chemical reactions involved in maintaining the living state of the cells and the organism.
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Heidelberg Forum 2013

There was much interest in the booth of antibodies-online GmbH at the “3rd Heidelberg Forum for Young Life Scientists 2013”. This forum, sponsored by antibodies-online, took place in Heidelberg on June 6th to 7th 2013. Numerous PhD candidates from the life science sector as well as scientists from Germany and abroad took advantage of the forum that focused on Biotechnology, OMICS, Infection and Immunity as well as Stem Cell Research to present and discuss their research.  » 
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