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Cadherin: New monoclonal antibodies available
Area: Cell Structure
Cadherins constitute a family of transmembrane glycoproteins involved in Calcium-dependent cell interactions. Cadherins play important roles in cell adhesion, forming desmosomes and ensuring that cells within tissues are connceted.
The Clone 5H9 derived antibody is suitable for immunoblotting, immunocytochemistry and immunohistochemistry.
Amyloid beta: Antibody for research on Alzheimer's disease
Area: Alzheimer's Disease
Assemblies of the Amyloid-β peptide are thought to be the direct cause for the Alzheimer pathology. Two recently published antibodies for Amyloid-beta are the anti-Aβ pE3 antibody against the N-terminally truncated Aβ for Immunohistochemistry (IHC), ELISA, and Western blotting, and a highly selectivity antibody against Aβ oligomers (a.a. 1-40) for IHC and ELISA.
TAG-antibodies: Anti-GFP/RFP antibodies in recent publications
Area: Transfection, Transfected cell culture
High-quality antibodies against fluorescent TAGs are for example an HRP-conjugated anti-GFP antibody that has recently been used in a JBC publication, as well as a monoclonal (3H9) anti-GFP antibody. Additionally, an anti-RFP antibody has recently been used in a BBRC publication.
Programmed Cell Death 4 (PDCD4): Recent publications in PLOS and Oncogene
Area: Apoptosis
Programmed cell death 4 (PDCD4) protein is localized to the nucleus in proliferating cells. PDCD4 is thought to play a role in apoptosis but the exact role has not yet been elucidated. Two recent publications in Oncogene and PLOS one utilized a polyclonal anti-PDCD4 antibody for studies in rodents. The antibody can be used for Western Blottin and ELISA assays.
Coagulation factors: Frequently used anti-von Willebrand Factor
Area: Blood Components
Von Willebrand factor (VWF) is a carrier protein that binds human FVIII procoagulant (FVIII) non-covalently. FVIII/VWF exists in plasma as a series of polymers with molecular weights larger than 1100 kD. A frequently purchased anti-VWF antibody is ABIN458118. The antiserum is standardized for electroimmunodiffusion (EID).
New antibodies against bovine Papillomavirus type-1 transactivator protein E2
Area: Virology
The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription.
A range of well published antibodies against various epitopes of bovine Paillomavirus type-1 transactivator protein E2 are now available on antibodies-online.com.
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Monoclonal anti-human IgE antibodies: Research tools for allergy research
Area: Allergy
Several new anti-human IgE antibodies for allergy research are now available from antibodies-online.com. Among them are the well published anti-human IgE antibody (SUS-11)
anti-human IgE antibody (BSW17) and
monoclonal anti-human IgE antibody (Le27).
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Western Blot: More than 136.000 secondary and primary antibodies available
Application: Western Blot (WB)
antibodies-online.com offers close to 200 alkaline phosphatase conjugate secondary antibodies and almost 300 HRP conjugated antibodies that can be used as secondary antibodies for Western Blotting. In total some 1300 secondary antibodies are available for Western Blotting. The number of primary antibodies for Western Blotting exceeds 135.000 products.
Western Blot: Positive and negative controls
Application: Western Blot (WB)
antibodies-online offers more than 500 peptides that can be used for blocking of primary antibodies in Western Blot analysis. Additionally, there are close to 1000 recombinant proteins that can be used as positive control, i.e. for evaluation of the signal intensity of the antibody with the purified target and a control of the location of the specific band of the target.
Loading controls for Western Blotting
Application: Western Blot (WB)
Loading controls are used to normalize the levels of protein detected by confirming that protein loading is the same in all lanes of the gel. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti-COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies. antibody-online also offers loading buffer for your samples.
Western Blot: Easy-to-use chemiluminescence substrates
Application: Western Blot (WB)
Mark-it Glow is a novel two component substrates for the development of Western Blots. Its base is the catalysis of luminol by horseradish peroxidase (HRP) conjugated antibodies. Additionally the Mark-it Glow Ultra formulation offers an extremely sensitive detection limit. Mark-it glow is not pre-mixed but the two reagents are sprayed separately onto the membrane.
Novel NBS-C products: anti-immungolublin E antibody
Area: Inflammation
antibodies-online.com offers products from NBS-C specialized on research of, for example, mast cell and basophil activation. The anti-IgE antibodies ABIN457503 and ABIN457500 can be used for inhibition of the IgE receptor for the research of cell signaling. Additionally, recombinant human IgE protein is available from NBS-C for protein concentration measurements.
Apoptosis research: Cell fractionation kit in current publication
Area: Apoptosis
antibodies-online.com offers various kits that help with basic techniques required for apoptosis investigations, such as the FractionPREP Cell Fractionation Kit that has been applied for instance in a 2010 publication of the Journal of Virology. Another example is the ready-to-use apoptosis inducer set, that contains 5 chemical reagents to induce apoptosis.
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ATP level determination: Mitochondria-mediated apoptosis
Area: Apoptosis
Apoptosis is an energy-consuming, ATP lowering process. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions the cell dies. Apoptotic cells hence exhibit significant decreases in ATP levels. An efficient way of detecting ATP changes is the ApoSENSOR High Throughput Apoptosis & Cell Viability Assay Kit that can be analyzed using luminometers or Beta Counters.
Caspase assay kits: Cytoplasm-mediated apoptosis
Area: Apoptosis
Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREEN Caspase Screening Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. Complementary products include the CaspGLOW Fluorescein Active Caspase Staining Kit, Caspase-1 Inhibitor Drug Screening Kit, Caspase-1 Fluorometric Assay Kit and many others.
Annexin V: Tools for the detection of membrane mediated apoptosis
Area: Apoptosis
Detection of Annexin V on cell-membranes offers an exciting possibility for the investigation of apoptosis in living cells. Using Annexin V antibodies labeled with fluorescent markers allows for the usage in fluorescence microscopy and flow cytometry. antibodies-online.com offers this ready-to-use Annexin V FITC labelling kit that gives results in only 10 minutes.
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ELISA kits: more than 100 images added
Standard curves have been added to more than 100 frequently used ELISA kit datasheets. Here you may find an overview of the kits: »Show ELISA kits
Extended product descriptions
Information regarding sensitivity, specificity and functional principle has been added to more than 100 product descriptions of ELISA kits. Here you may find a list of extended ELISA kit descriptions:
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New Xenotropic Murine Leukemia Virus gp70 antibody
Area: Virology
The murine leukemia virus (MuLV) is a positive sense, single-stranded RNA (ssRNA) retrovirus, which replicates via DNA intermediates using a reverse transcriptase. antibodies-online offers an anti-Xenotropic Murine Leukemia Virus gp70 antibody that can be used for ELISA, Western Blotting and Immunohistochemistry (IHC). The big advantage of this anti-MuLV antibody is, that it can recognize the virus not only in mouse, but also in human samples.
Quantitation standards for Western blotting in plant research
Area: Plant/Algal Physiology
Western blotting can show qualitative changes in the relative abundance of individual proteins. Via addition of quantitation standards to the Western blot samples the absolute amounts of a protein in a given sample can be evaluated. Using a dilution range of protein standards a concentration curve can be established that allows the measurement of concentrations of unknown protein samples. Quantitation standards for plant research can be found here: anti-Cp47 (Psbb) antibody und anti-Atpb protein antibody.
EIAab is a new supplier on antibodies-online.com
Supplier: Wuhan EIAab Science
Wuhan EIAab Science is now a supplier on antibodies-online. EIAab provides close to 3000 ELISA kits for measurement of concentration of a great variety of targets. Due to the addition of EIAab's USCNLIFE products to the list of ELISA kits available on antibodies-online.com our portfolio has grown to over 31.000 ELISA kits for target proteins and small molecular substances. The new products can be found here: show all ELISA kit products from EIAab Science.
Albumin ELISA Kit in recent publications
Area: Experimental Models
antibodies-online offers a newly added anti-Albumin ELISA kit that has been used in recent publications. Albumin serves as a marker in monitoring kidney damages. Animal models are frequently employed when examining nephrotoxic substances and therapeutic strategies regarding kidney damages. For estimation of kidney function and quantification of albumin in mouse urine, this anti-Albumin ELISA kit is a premier choice.
CellTrend added as new supplier on antibodies-online.com
Supplier: CellTrend
CellTrend is an exciting new addition to the list of suppliers of ELISA kits and other products on antibodies-online.com. CellTrend provides enzyme linked immuno sorbend assays (ELISA) kits. Examples of high quality CellTrend products include autoantibody ELISA detection kits for Angiotensin II Receptor 1 antibodies, Endothelin Receptor A antibodies, b2 adrenergic Receptor antibodies, PAR1 antibodies and Albumin antibodies.
Interesting antibodies in publications of the past three months
Special: Published antibodies
Antibodies available on antibodies-online that have been used in publications in the past 10 weeks: anti-Cathepsin and anti-BCL2 antibodies in cancer research, CoxIIb antibody in research on photosynthesis, anti-PPARalpha antibody in research on apoptosis, anti-SIRT3 antibody in research on oxidative stress and many more. Here you may find a complete list of recently published antibodies.
New kit for a more efficient extraction of membrane proteins
Application: Biochemical Assays
antibodies-online offers a Membrane Protein Extraction Kit for effective extraction of membrane proteins from mammalian tissues and cells in less than one hour. Unlike other available procedures this kit does not only extract the total cellular membrane proteins, but also the plasma membrane proteins, with a consistently high yield and purity of over 90%.
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