DNA Oxidation is Triggered by H3K9me2 Demethylation and Drives Estrogen-Induced Gene Expression

In a recent paper, Bruno Perillo and Maria Neve Ombra from the Instituto di Scienze dell'Alimentazione in Italy present a strategy that uses controlled DNA damage and repair to guide sites of gene transcription.

Efficient in vivo transcription is accompanied by N-terminal modifications of nucleosomal histones. The scientists analyzed how estrogen-responsive gene expression is controlled by H3 histone methylation and demethylation.They demonstrate that an estrogen receptor bound to DNA can regulate transcription by participating in bending chromatin to contact the RNA polymerase II recruited to the promoter. In this process, LSD1 demethylase causes receptor-targeted demethylation of H3 lysine 9 at both enhancer and promoter sites. The demethylation process produces hydrogen peroxide and modifies the surrounding DNA. 8-oxoguanine-DNA glycosylase 1 and topoisomerase II-β are recruited, whereby chromatin and DNA undergo conformational changes that are essential for estrogen-induced gene transcription.

Related antibodies on antibodies-online.com:

Histon H3 Lysin 9 (H3K9)

RNA Polymerase II

Estrogen receptor

LSD1

8-oxoguanine-DNA glykosylase 1 (OGG1)

OGG1

Topoisomerase II-β

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