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anti-Dog (Canine) CD40 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Reactivity
Dog (Canine), Human
622
307
69
19
14
10
9
6
4
3
2
2
1
1
1
Host
Mouse
491
208
158
23
4
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
87
84
49
24
22
21
17
15
13
13
12
9
9
9
9
9
9
9
8
7
7
7
7
6
6
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
559
231
199
152
105
102
91
62
45
33
19
15
13
9
8
6
6
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN337317
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1822271 IHC (fro) IHC (p) IP WB Rabbit IgG Cytoplasmic Domain Log in to see Polyclonal
1 ABIN2480305 IHC (fro) FACS IHC (p) IP Mouse IgG2a Log in to see LOB7-6 4
1 ABIN2618536 FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see LOB7-6
1 ABIN2480314 IHC (fro) FACS IHC (p) IP Mouse IgG2a Log in to see LOB7-6 4

Top referenced anti-CD40 antibody for Immunohistochemistry (Frozen Sections)

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Application / Reactivity Human
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ELISA 156 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 338 Antibodies
Functional Studies (Func) 21 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 29 Antibodies
Immunofluorescence (IF) 56 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 119 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 65 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Dog (Canine), Human
(622), (307), (69), (19), (14), (10), (9), (6), (4), (3), (2), (2), (1), (1), (1)
Host Mouse
(491), (208), (158), (23), (4), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(87), (84), (49), (24), (22), (21), (17), (15), (13), (13), (12), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(559), (231), (199), (152), (105), (102), (91), (62), (45), (33), (19), (15), (13), (9), (8), (6), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes the human CD40 cell surface antigen, a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centers and is vital in T cell-B cell interactions.
Purification Protein A purified
Clone LOB7-6
Isotype IgG2a

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background Name/Gene ID: CD40
Family: TNF Receptor

Synonyms: CD40, B-cell surface antigen CD40, B cell-associated molecule, B cell surface antigen CD40, CD40 antigen, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TNFRSF5
Gene ID 958
UniProt P25942
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling Images back to top
Application Notes Approved: Flo (20 μg/mL), IHC-Fr (1 - 10 μg/mL), IHC-P (10 μg/mL), IP

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining e. g. pronase or trypsin. Histology Positive Control Tissue: Human Tonsil.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Tris buffered saline, 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Store undiluted.
Storage 4 °C,-20 °C
Storage Comment +4°C or -20°C, Avoid repeated freezing and thawing. Store undiluted.

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5) (ABIN337317) Human Spleen (formalin-fixed, paraffin-embedded) stained with CD40 antibody ABIN33731...