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anti-Human CD40 antibody for ELISA

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • 2310042E05Rik
  • AI326936
  • Bp50
  • C87049
  • C130077D06Rik
  • CDW40
  • DCTN-50
  • GMP23-48K
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • p50
  • RBP50
  • T-BAM
  • Tnfrsf5
  • TNFRSF5
  • TNFSF5
  • TRAP
Epitope
AA 1-152
61
37
31
28
22
19
15
8
7
7
5
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
605
302
57
13
11
8
7
5
4
2
1
1
1
1
1
Host
Mouse
481
211
154
24
9
2
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
95
91
52
24
20
17
14
12
12
11
9
8
8
8
8
8
8
8
8
8
7
6
6
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
567
199
197
107
88
78
72
50
48
21
19
11
8
8
6
4
3
3
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN394072
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32693866 ABIN560250 ELISA WB Mouse IgG2b kappa AA 1-151, full length Log in to see 1G1
0.0012117105 ABIN1531702 ELISA WB Rabbit IgG pThr254 Log in to see Polyclonal 1
0.0012117105 ABIN969025 ICC ELISA Mouse IgG1 Log in to see 9G10 2
0.0012117105 ABIN610077 Func FACS IP ELISA PE Mouse IgG1 Log in to see BE-1 3
0.0012117105 ABIN610290 Func FACS ELISA Biotin Mouse IgG1 Log in to see EA-5 2
0.0012117105 ABIN610291 Func FACS ELISA FITC Mouse IgG1 Log in to see EA-5 2
0.0012117105 ABIN1533424 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2660582 IHC ELISA WB APC-Cy7 Mouse IgG1 kappa Log in to see 5C3 5
0.0012117105 ABIN467092 IF ELISA Mouse IgG1 Log in to see 9G10
0.0012117105 ABIN610074 Func FACS IP ELISA Mouse IgG1 Log in to see BE-1 2
0.0012117105 ABIN2889270 ELISA WB Rabbit IgG AA 228-277 Log in to see Polyclonal
0.0012117105 ABIN610078 Func FACS IP ELISA Mouse IgG1 Log in to see BE-1 3
0.0012117105 ABIN203954 FACS IP ELISA Biotin Mouse IgG1 Log in to see
0.0012117105 ABIN2462500 ELISA WB Rabbit Log in to see Polyclonal
0.0012117105 ABIN2458616 ELISA WB Rabbit Log in to see Polyclonal
0.0012117105 ABIN2163426 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0012117105 ABIN610075 Func FACS IP ELISA Biotin Mouse IgG1 Log in to see BE-1
0.0012117105 ABIN610076 Func FACS IP ELISA FITC Mouse IgG1 Log in to see BE-1
0.0012117105 ABIN610292 Func FACS ELISA PE Mouse IgG1 Log in to see EA-5
0.0012117105 ABIN2163425 ELISA WB Rabbit IgG Thr254 Log in to see Polyclonal

Top referenced anti-CD40 antibody for ELISA

Similar anti-CD40 Antibodies

Application / Reactivity Human
ELISA 155 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 340 Antibodies
Functional Studies (Func) 23 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 17 Antibodies
Immunofluorescence (IF) 44 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 63 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 69 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 70 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunoprecipitation (IP) 53 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope AA 1-152
(61), (37), (31), (28), (22), (19), (15), (8), (7), (7), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(605), (302), (57), (13), (11), (8), (7), (5), (4), (2), (1), (1), (1), (1), (1)
Host Mouse
(481), (211), (154), (24), (9), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(95), (91), (52), (24), (20), (17), (14), (12), (12), (11), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(567), (199), (197), (107), (88), (78), (72), (50), (48), (21), (19), (11), (8), (8), (6), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN394072) Images
Specificity CD40 (AAH64518, 1 a.a. ~ 152 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen CD40 (AAH64518, 1 a.a. ~ 152 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1G1
Isotype IgG2b kappa

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN394072) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 30619 DA
Gene ID 12406
Research Area Cell Signaling, Transcription Factors, Signaling, Cell Structure
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN394072) Images back to top
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN394072) Images back to top
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-CD40 Antibody (ABIN394072)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Soliman, Fathy, Alkilani, Abd El-Bary, El-Bassal: "Impact of CD40 expression by flowcytometry on outcome of patients with non-Hodgkin's lymphoma." in: The Egyptian journal of immunology / Egyptian Association of Immunologists, Vol. 16, Issue 1, pp. 61-70, 2010

Rodríguez-Rodríguez, Castañeda, Vázquez-Rodríguez, Morado, Marí-Alfonso, Gómez-Vaquero, Miranda-Filloy, Narvaez, Ortego-Centeno, Blanco, Fernández-Gutiérrez, Martín, González-Gay: "Influence of CD40 rs1883832 polymorphism in susceptibility to and clinical manifestations of biopsy-proven giant cell arteritis." in: The Journal of rheumatology, Vol. 37, Issue 10, pp. 2076-80, 2010

Lewis, Palmer, Ellington, Divers, Ng, Lu, Langefeld, Freedman, Bowden: "Analysis of candidate genes on chromosome 20q12-13.1 reveals evidence for BMI mediated association of PREX1 with type 2 diabetes in European Americans." in: Genomics, Vol. 96, Issue 4, pp. 211-9, 2010

Blanco-Kelly, Matesanz, Alcina, Teruel, Díaz-Gallo, Gómez-García, López-Nevot, Rodrigo, Nieto, Cardeña, Alcain, Díaz-Rubio, de la Concha, Fernandez, Arroyo, Martín, Urcelay: "CD40: novel association with Crohn's disease and replication in multiple sclerosis susceptibility." in: PLoS ONE, Vol. 5, Issue 7, pp. e11520, 2010

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN394072) back to top
Supplier Images
ELISA image for anti-CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5) (AA 1-152) (ABIN394072) Detection limit for recombinant GST tagged CD40 is approximately 0.1 ng/mL as a captu...