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anti-Human CD40 antibody for Intracellular Flow Cytometry

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Reactivity
Human
625
307
69
20
14
10
9
6
4
3
2
2
1
1
1
Host
Mouse
491
208
159
23
6
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is conjugated to Alexa Fluor 488
87
84
49
24
22
20
17
15
13
13
12
9
9
9
9
9
9
9
8
7
7
7
7
6
6
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
559
234
200
152
106
103
92
62
45
33
18
15
13
9
8
6
6
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2657426
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2661710 ICFC FITC Mouse IgG1 kappa Log in to see 5C3 4
1 ABIN2663450 ICFC PE Mouse IgG1 kappa Log in to see 5C3 5
1 ABIN2658656 FACS ICFC APC Mouse IgG1 kappa Log in to see 5C3 4
1 ABIN2658658 FACS ICFC APC Mouse IgG1 kappa Log in to see HB14 8
1 ABIN2661711 ICFC FITC Mouse IgG1 kappa Log in to see 5C3 4
1 ABIN2663451 ICFC PE Mouse IgG1 kappa Log in to see 5C3 5
1 ABIN2658657 FACS ICFC APC Mouse IgG1 kappa Log in to see 5C3 4

Top referenced anti-CD40 antibody for Intracellular Flow Cytometry

Similar anti-CD40 Antibodies

Application / Reactivity Human
Agonist (Agon) 2 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
ELISA 157 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 338 Antibodies
Functional Studies (Func) 21 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 29 Antibodies
Immunofluorescence (IF) 56 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 119 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 65 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Human
(625), (307), (69), (20), (14), (10), (9), (6), (4), (3), (2), (2), (1), (1), (1)
Host Mouse
(491), (208), (159), (23), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is conjugated to Alexa Fluor 488
(87), (84), (49), (24), (22), (20), (17), (15), (13), (13), (12), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
(559), (234), (200), (152), (106), (103), (92), (62), (45), (33), (18), (15), (13), (9), (8), (6), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN2657426) Images
Cross-Reactivity Baboon, Chimpanzee, Cynomolgus, Rhesus Monkey, Squirrel Monkey (Saimiri spec.)
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Clone 5C3
Isotype IgG1 kappa

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN2657426) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN2657426) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN2657426) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-CD40 Antibody (ABIN2657426)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Pound, Challa, Holder, Armitage, Dower, Fanslow, Kikutani, Paulie, Gregory, Gordon: "Minimal cross-linking and epitope requirements for CD40-dependent suppression of apoptosis contrast with those for promotion of the cell cycle and homotypic adhesions in human B cells." in: International immunology, Vol. 11, Issue 1, pp. 11-20, 1999

Foy, Aruffo, Bajorath, Buhlmann, Noelle: "Immune regulation by CD40 and its ligand GP39." in: Annual review of immunology, Vol. 14, pp. 591-617, 1996

Banchereau, Bazan, Blanchard, Brière, Galizzi, van Kooten, Liu, Rousset, Saeland: "The CD40 antigen and its ligand." in: Annual review of immunology, Vol. 12, pp. 881-922, 1994

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN2657426) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5)  (Alexa Fluor 488) (ABIN2657426) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) antibody (Alexa Fluor 488)