anti-Human CD40 antibody for Indirect Immunofluorescence Assay

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Reactivity
Human
647
312
69
20
14
10
10
6
4
3
2
2
1
1
1
1
Host
Mouse
505
213
169
23
6
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
88
85
51
24
24
21
17
15
14
13
12
9
9
9
9
9
9
9
8
7
7
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Indirect Immunofluorescence Assay (IFA)
579
245
207
157
111
103
101
65
47
34
19
15
13
13
9
6
6
4
3
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN234620
$ 706.93
Plus shipping costs $45.00

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Human
(647), (312), (69), (20), (14), (10), (10), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(505), (213), (169), (23), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(88), (85), (51), (24), (24), (21), (17), (15), (14), (13), (12), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Indirect Immunofluorescence Assay (IFA)
(579), (245), (207), (157), (111), (103), (101), (65), (47), (34), (19), (15), (13), (13), (9), (6), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling Images
Specificity CD40 is a 49 kDa type I integral membrane protein of the NGF receptor family. CD40 is found on normal and neoplastic B cells.(1) It is a pan-B marker, absent only from terminally differentiated plasma cells. CD40 is predicted to function in the process of B cell selection in the germinal center. Studies demonstrated that the antibody induces strong homotypic adhesions in resting B cells and, together with Interleukin-4 (IL-4) maintains the cell cycle of B blasts and promotes the switch to IgE secretion. (1,2) Purified B cells cultured in the CD40 system in the presence of IL-10 differentiate into plasma cells and secrete large amounts of immunoglobulins. (3) The ligand for CD40 is a membrane glycoprotein on activated T cells that induces B cell proliferation and immunoglobulin secretion. (4) This clone acts in synergy with IL-4 to induce the increase of B-cell volume and the proliferation of anti IgM antibody-co-stimulated or pre-activated B cells. (5)
Characteristics MAb to CD40 B cells, Monoclonal antibody to CD40 B cells
Purification Protein A Chromatography
Immunogen Anti-mu activated tonsillar human B cells
Clone MAB89
Isotype IgG1

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD40 B Cells (CD40 Antibody Abstract)
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling Images back to top
Application Notes Flow cytometry Study of B-cell proliferation, B-cell growth and activation factors Functional study of B-cell subpopulations. Fluorescent microscopy or flow cytometry: 2 µg/5x105 cells/test Activation assays: Working concentration: 10 ng/mL Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute with 1 mL of distilled water.
Buffer Lyophilized from PBS containing 1 mg/mL BSA
Preservative Without preservative
Handling Advice Avoid multiple freeze/thaw cycles. The addition of 0.1 % (w/v) sodium azide is recommended for storage of the Reconstituted form for up to one month at 2-8 °C.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized product at 2-8 °C. After reconstitution, aliquot and store at -28 °C.