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|Antigen||CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies|
|Conjugate||This CD40 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
|2 references available|
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Product Details anti-CD40 AntibodyTarget Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN118631) Images
|Specificity||This antibody recognises the CD40 cell surface antigen.|
|Purification||Affinity chromatography on Protein A.|
|Immunogen||Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.|
Target Details CD40Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN118631) Images back to top
|Alternative Name||CD40 (CD40 Antibody Abstract)|
|Background||CD40 is a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centres, and is vital in T cell-B cell interactions.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5|
|Research Area||Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer|
|Pathways||NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN118631) Images back to top
Flow Cytometry: 20 μg/mL, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen sections: 1-10 μg/mL. Immunohistochemistry on Paraffin sections: 1-10 μg/mL. This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining eg. pronase or trypsin. Recommended Positive Control: Human Tonsil. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN118631) Images back to top
|Buffer||Tris buffered saline containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD40 antibody (ABIN118631)Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Kirsch, Zeyda, Stuhlmeier, Grisar, Smolen, Watschinger, Stulnig, Hörl, Zlabinger, Säemann: "The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function." in: Arthritis research & therapy, Vol. 7, Issue 3, pp. R694-703, 2005
Quadbeck, Eckstein, Tews, Walz, Hoermann, Mann, Gieseler: "Maturation of thyroidal dendritic cells in Graves' disease." in: Scandinavian journal of immunology, Vol. 55, Issue 6, pp. 612-20, 2002