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anti-Human CD40 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Reactivity
Human
633
315
67
21
15
12
11
8
4
3
2
2
1
1
1
1
Host
Mouse
499
214
160
23
4
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
95
92
53
21
21
18
15
13
13
12
12
9
9
9
9
9
9
9
8
7
6
6
6
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
572
235
204
153
104
102
94
63
46
33
19
13
13
9
8
8
6
6
4
3
3
3
3
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN118631
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1106354 Func IHC (fro) FACS Mouse IgG1 Log in to see B-B20 3
1 ABIN111321 Func FACS IHC (fro) IHC (p) Mouse IgG1 Log in to see MAB89 4
1 ABIN319728 FACS IHC (fro) IP Mouse IgG2a Log in to see LOB7-6 2
1 ABIN2451934 IHC (fro) FACS ISt Biotin Mouse IgG1 kappa Extracellular Domain Log in to see 5C3 3
1 ABIN2451935 IHC (fro) FACS FITC Mouse IgG1 kappa Extracellular Domain Log in to see 5C3 3
1 ABIN1383854 IHC (fro) FACS Mouse IgG1 Log in to see B-B20 4
1 ABIN1106355 IHC (fro) IF Mouse IgM Log in to see BL-C4
1 ABIN317137 EIA IHC (fro) WB Mouse IgG1 Log in to see 3B2
1 ABIN337317 FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see LOB7-6
1 ABIN1804378 ICC IHC (fro) FACS IHC (p) IP Mouse IgG2a Log in to see
1 ABIN2689245 IHC (fro) FACS IHC (zinc) IHC (p) Mouse IgG1, kappa Log in to see 5C3 2
1 ABIN1822271 IHC (fro) IHC (p) IP WB Rabbit IgG Cytoplasmic Domain Log in to see Polyclonal
1 ABIN2480305 IHC (fro) FACS IHC (p) IP Mouse IgG2a Log in to see LOB7-6 4
1 ABIN1722607 FACS Func IHC (fro) WB Mouse IgG1 Log in to see B-B20
1 ABIN203956 FACS IHC (fro) Biotin Mouse IgG1 Log in to see
1 ABIN476171 FACS IHC (fro) Mouse IgG1, kappa Log in to see 5C3
1 ABIN600790 IHC (fro) IHC (p) ELISA WB Mouse IgG1 Log in to see
1 ABIN2689243 IHC (fro) FACS Mouse IgG1, kappa Log in to see 5C3 2
1 ABIN2618536 FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see LOB7-6
1 ABIN203953 FACS IHC (fro) IP Mouse IgG1 Log in to see

Top referenced anti-CD40 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CD40 Antibodies

Application / Reactivity Human
Agonist (Agon) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
ELISA 158 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 347 Antibodies
Functional Studies (Func) 22 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 29 Antibodies
Immunofluorescence (IF) 57 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 120 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 65 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 104 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Human
(633), (315), (67), (21), (15), (12), (11), (8), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(499), (214), (160), (23), (4), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(95), (92), (53), (21), (21), (18), (15), (13), (13), (12), (12), (9), (9), (9), (9), (9), (9), (9), (8), (7), (6), (6), (6), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(572), (235), (204), (153), (104), (102), (94), (63), (46), (33), (19), (13), (13), (9), (8), (8), (6), (6), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN118631) Images
Specificity This antibody recognises the CD40 cell surface antigen.
Purification Affinity chromatography on Protein A.
Immunogen Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone LOB7-6
Isotype IgG2a

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN118631) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centres, and is vital in T cell-B cell interactions.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5
Gene ID 958
NCBI Accession NP_001241
UniProt P25942
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN118631) Images back to top
Application Notes Flow Cytometry: 20 μg/mL, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen sections: 1-10 μg/mL. Immunohistochemistry on Paraffin sections: 1-10 μg/mL. This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining eg. pronase or trypsin. Recommended Positive Control: Human Tonsil. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN118631) Images back to top
Concentration 1.0 mg/mL
Buffer Tris buffered saline containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD40 Antibody (ABIN118631)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Kirsch, Zeyda, Stuhlmeier, Grisar, Smolen, Watschinger, Stulnig, Hörl, Zlabinger, Säemann: "The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function." in: Arthritis research & therapy, Vol. 7, Issue 3, pp. R694-703, 2005

Quadbeck, Eckstein, Tews, Walz, Hoermann, Mann, Gieseler: "Maturation of thyroidal dendritic cells in Graves' disease." in: Scandinavian journal of immunology, Vol. 55, Issue 6, pp. 612-20, 2002