anti-Human CD40 antibody for Immunoprecipitation

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Reactivity
Human
647
312
69
20
14
10
10
6
4
3
2
2
1
1
1
1
Host
Mouse
505
213
169
23
6
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
88
85
51
24
24
21
17
15
14
13
12
9
9
9
9
9
9
9
8
7
7
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
579
245
207
157
110
102
100
65
47
34
19
15
13
13
9
6
6
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN118631
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN319728 FACS IHC (fro) IP Mouse IgG2a Log in to see LOB7-6 2
1 ABIN610077 Func FACS IP ELISA PE Mouse IgG1 Log in to see BE-1 3
1 ABIN610074 Func FACS IP ELISA Mouse IgG1 Log in to see BE-1 2
1 ABIN610078 Func FACS IP ELISA Mouse IgG1 Log in to see BE-1 3
1 ABIN2480305 IHC (fro) FACS IHC (p) IP Mouse IgG2a Log in to see LOB7-6 4
1 ABIN4293028 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1
1 ABIN784060 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN625924 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2470676 FACS IP IHC Mouse IgG2a Log in to see LOB7-6
1 ABIN337317 FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see LOB7-6
1 ABIN2118316 ICC IHC IP WB Rabbit IgG AA 26-187 Log in to see Polyclonal
1 ABIN610075 Func FACS IP ELISA Biotin Mouse IgG1 Log in to see BE-1
1 ABIN1732862 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1804378 ICC IHC (fro) FACS IHC (p) IP Mouse IgG2a Log in to see
1 ABIN203954 FACS IP ELISA Biotin Mouse IgG1 Log in to see
1 ABIN2480314 IHC (fro) FACS IHC (p) IP Mouse IgG2a Log in to see LOB7-6 4
1 ABIN1495123 IP WB Sepharose Rabbit N-Term Log in to see Polyclonal
1 ABIN1495122 IP WB Magnetic Particles Rabbit N-Term Log in to see Polyclonal
1 ABIN1822271 IHC (fro) IHC (p) IP WB Rabbit IgG Cytoplasmic Domain Log in to see Polyclonal
1 ABIN2226260 FACS IP ELISA PE Mouse IgG1 Log in to see 7H162

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Human
(647), (312), (69), (20), (14), (10), (10), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(505), (213), (169), (23), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(88), (85), (51), (24), (24), (21), (17), (15), (14), (13), (12), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(579), (245), (207), (157), (110), (102), (100), (65), (47), (34), (19), (15), (13), (13), (9), (6), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling ProductDetails: References for anti-CD40 antibody (ABIN118631) Images
Specificity This antibody recognises the CD40 cell surface antigen.
Purification Affinity chromatography on Protein A.
Immunogen Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone LOB7-6
Isotype IgG2a

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling ProductDetails: References for anti-CD40 antibody (ABIN118631) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centres, and is vital in T cell-B cell interactions.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5
Gene ID 958
NCBI Accession NP_001241
UniProt P25942
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling ProductDetails: References for anti-CD40 antibody (ABIN118631) Images back to top
Application Notes Flow Cytometry: 20 μg/mL, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen sections: 1-10 μg/mL. Immunohistochemistry on Paraffin sections: 1-10 μg/mL. This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining eg. pronase or trypsin. Recommended Positive Control: Human Tonsil. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details ProductDetails: References for anti-CD40 antibody (ABIN118631) Images back to top
Concentration 1.0 mg/mL
Buffer Tris buffered saline containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD40 antibody (ABIN118631)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Kirsch, Zeyda, Stuhlmeier, Grisar, Smolen, Watschinger, Stulnig, Hörl, Zlabinger, Säemann: "The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function." in: Arthritis research & therapy, Vol. 7, Issue 3, pp. R694-703, 2005

Quadbeck, Eckstein, Tews, Walz, Hoermann, Mann, Gieseler: "Maturation of thyroidal dendritic cells in Graves' disease." in: Scandinavian journal of immunology, Vol. 55, Issue 6, pp. 612-20, 2002