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anti-Human CD40 antibody for Immunoprecipitation

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • 2310042E05Rik
  • AI326936
  • Bp50
  • C87049
  • C130077D06Rik
  • CDW40
  • DCTN-50
  • GMP23-48K
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • p50
  • RBP50
  • T-BAM
  • Tnfrsf5
  • TNFRSF5
  • TNFSF5
  • TRAP
Reactivity
Human
447
262
55
11
11
8
7
5
4
2
1
1
1
1
Host
Mouse
348
175
130
24
7
1
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
82
80
43
24
9
8
7
6
6
6
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
417
155
140
79
61
49
48
46
45
19
19
11
8
8
6
4
3
3
2
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN118631
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008919076 ABIN319728 FACS IHC (fro) IP Mouse IgG2a Log in to see LOB7-6 2
0.0008919076 ABIN610077 Func FACS IP ELISA PE Mouse IgG1 Log in to see BE-1 3
0.0008919076 ABIN610074 Func FACS IP ELISA Mouse IgG1 Log in to see BE-1 2
0.0008919076 ABIN784060 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008919076 ABIN625924 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008919076 ABIN610078 Func FACS IP ELISA Mouse IgG1 Log in to see BE-1 3
0.0008919076 ABIN337317 FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see LOB7-6
0.0008919076 ABIN203954 FACS IP ELISA Biotin Mouse IgG1 Log in to see
0.0008919076 ABIN610075 Func FACS IP ELISA Biotin Mouse IgG1 Log in to see BE-1
0.0008919076 ABIN610076 Func FACS IP ELISA FITC Mouse IgG1 Log in to see BE-1
0.0008919076 ABIN2470676 FACS IP IHC Mouse IgG2a Log in to see LOB7-6
0.0008919076 ABIN1822271 IHC (fro) IHC (p) IP WB Rabbit IgG Cytoplasmic Domain Log in to see Polyclonal
0.0008919076 ABIN234621 IHC (f) FACS IHC (p) IP Mouse IgG2a Log in to see LOB7-6 1
0.0008919076 ABIN1804378 ICC IHC (fro) FACS IHC (p) IP Mouse IgG2a Log in to see
0.0008919076 ABIN2164926 IHC (af) IHC (fp) FACS IP Mouse IgG2a Log in to see
0.0008919076 ABIN1823221 FACS IP IHC Mouse IgG2a Log in to see LOB7-6
0.0008919076 ABIN2166683 IHC (af) IHC (fp) FACS IP Mouse IgG2a Log in to see
0.0008919076 ABIN2166684 IHC (af) IHC (fp) FACS IP Mouse IgG2a Log in to see
0.0008919076 ABIN2480305 IHC (fro) FACS IHC (p) IP Mouse IgG2a Log in to see LOB7-6 4
0.0008919076 ABIN1732862 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-CD40 antibody for Immunoprecipitation

Similar anti-CD40 Antibodies

Application / Reactivity Human
ELISA 116 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 232 Antibodies
Functional Studies (Func) 21 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 43 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 49 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 31 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunoprecipitation (IP) 45 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Human
(447), (262), (55), (11), (11), (8), (7), (5), (4), (2), (1), (1), (1), (1)
Host Mouse
(348), (175), (130), (24), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(82), (80), (43), (24), (9), (8), (7), (6), (6), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(417), (155), (140), (79), (61), (49), (48), (46), (45), (19), (19), (11), (8), (8), (6), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN118631) Images
Specificity This antibody recognises the CD40 cell surface antigen.
Purification Affinity chromatography on Protein A.
Immunogen Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone LOB7-6
Isotype IgG2a

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN118631) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centres, and is vital in T cell-B cell interactions.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5
Gene ID 958
NCBI Accession NP_001241
UniProt P25942
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN118631) Images back to top
Application Notes Flow Cytometry: 20 μg/mL, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen sections: 1-10 μg/mL. Immunohistochemistry on Paraffin sections: 1-10 μg/mL. This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining eg. pronase or trypsin. Recommended Positive Control: Human Tonsil. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN118631) Images back to top
Concentration 1.0 mg/mL
Buffer Tris buffered saline containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD40 Antibody (ABIN118631)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Kirsch, Zeyda, Stuhlmeier, Grisar, Smolen, Watschinger, Stulnig, Hörl, Zlabinger, Säemann: "The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function." in: Arthritis research & therapy, Vol. 7, Issue 3, pp. R694-703, 2005

Quadbeck, Eckstein, Tews, Walz, Hoermann, Mann, Gieseler: "Maturation of thyroidal dendritic cells in Graves' disease." in: Scandinavian journal of immunology, Vol. 55, Issue 6, pp. 612-20, 2002