anti-Human CD40 antibody for Immunostaining

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
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  • HIGM1
  • IGM
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Epitope
Extracellular Domain
55
52
46
26
24
22
19
19
18
8
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
658
331
69
20
14
10
10
6
4
3
2
2
1
1
1
1
Host
Mouse
514
231
171
23
6
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is conjugated to Biotin
87
86
49
27
25
24
20
18
14
13
12
10
10
10
9
9
9
9
9
9
9
8
7
6
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunostaining (ISt)
608
247
207
157
112
103
102
65
47
36
19
18
15
13
10
8
6
6
3
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN2451934
$ 401.50
Plus shipping costs $45.00

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope Extracellular Domain
(55), (52), (46), (26), (24), (22), (19), (19), (18), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(658), (331), (69), (20), (14), (10), (10), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(514), (231), (171), (23), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is conjugated to Biotin
(87), (86), (49), (27), (25), (24), (20), (18), (14), (13), (12), (10), (10), (10), (9), (9), (9), (9), (9), (9), (9), (8), (7), (6), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunostaining (ISt)
(608), (247), (207), (157), (112), (103), (102), (65), (47), (36), (19), (18), (15), (13), (10), (8), (6), (6), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN2451934) Images
Sterility Sterile filtered
Immunogen Recombinant extracellular domain of CD40
Clone 5C3
Isotype IgG1 kappa

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN2451934) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a 45-50- kDa glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. CD40 is specifically expressed on the surface of B cells and specialized antigen-presenting cells such as dendritic cells and macrophages. CD40 interacts with the CD40 ligand (CD154) which is found primarily on T cells, playing a role in both humoral and cell-mediated immune responses. Activation of CD40 on B cells by CD40 ligand causes B cell proliferation, differentiation, immunoglobulin isotype switching, germinal center formation, and stimulation of the humoral memory response. This antibody reacts with a 45-48 kDa type I integral membrane glycoprotein present on peripheral blood and tonsillar B cells, but not expressed on terminally differentiated B cells. The antibody against human CD40 was produced from hybridoma (5C3) cultured in serum-free medium and purified under mild conditions by proprietary chromatography processes.
UniProt P25942
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN2451934) Images back to top
Application Notes 1. Flowcytometry
2. Immunohistochemistry (acetone-fixed fronzen section,indirect immuno-staining)
Comment

Conjugate: [Biotin] / [IgG] = 7.1

Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN2451934) Images back to top
Format Liquid
Concentration 0.75 mg/mL
Buffer PBS, 50 % glycerol
Preservative Azide free
Storage -20 °C

References for anti-CD40 antibody (ABIN2451934)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Product cited in:

Ishida, Kumanogoh, Suzuki, Akahani, Noda, Kikutani: "Involvement of CD100, a lymphocyte semaphorin, in the activation of the human immune system via CD72: implications for the regulation of immune and inflammatory responses." in: International immunology, Vol. 15, Issue 8, pp. 1027-34, 2003

Yasui, Muraoka, Takaoka-Shichijo, Ishida, Takegahara, Uchida, Kumanogoh, Suematsu, Suzuki, Kikutani: "Dissection of B cell differentiation during primary immune responses in mice with altered CD40 signals." in: International immunology, Vol. 14, Issue 3, pp. 319-29, 2002

Inui, Kaisho, Kikutani, Stamenkovic, Seed, Clark, Kishimoto: "Identification of the intracytoplasmic region essential for signal transduction through a B cell activation molecule, CD40." in: European journal of immunology, Vol. 20, Issue 8, pp. 1747-53, 1990

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN2451934) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (Extracellular Domain) antibody (Biotin) (ABIN2451934) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (Extracellular Domain) antibody (Biotin)