anti-Human CD40 antibody for Stimulation

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Epitope
Extracellular Domain
55
52
46
26
24
22
19
19
18
8
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
658
331
69
20
14
10
10
6
4
3
2
2
1
1
1
1
Host
Mouse
514
231
171
23
6
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
87
86
50
27
25
24
20
18
14
13
12
10
10
10
9
9
9
9
9
9
9
8
7
6
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Stimulation (St), Immunohistochemistry (IHC)
608
247
207
156
112
103
103
65
47
36
19
18
15
13
10
8
6
6
3
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN2451933
$ 401.50
Plus shipping costs $45.00

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope Extracellular Domain
(55), (52), (46), (26), (24), (22), (19), (19), (18), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(658), (331), (69), (20), (14), (10), (10), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(514), (231), (171), (23), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(87), (86), (50), (27), (25), (24), (20), (18), (14), (13), (12), (10), (10), (10), (9), (9), (9), (9), (9), (9), (9), (8), (7), (6), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Stimulation (St), Immunohistochemistry (IHC)
(608), (247), (207), (156), (112), (103), (103), (65), (47), (36), (19), (18), (15), (13), (10), (8), (6), (6), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN2451933) Images
Purification Purified from serum-free medium by salting-out and ion-exchange chromatography under mild conditions.
Sterility Sterile filtered
Immunogen Recombinant extracellular domain of CD40 Product, Purified from serum-free medium by salting-out and ion-exchange chromatography under mild conditions.
Clone 5C3
Isotype IgG1 kappa

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN2451933) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a 45-50- kDa glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. CD40 is specifically expressed on the surface of B cells and specialized antigen-presenting cells such as dendritic cells and macrophages. CD40 interacts with the CD40 ligand (CD154) which is found primarily on T cells, playing a role in both humoral and cell-mediated immune responses. Activation of CD40 on B cells by CD40 ligand causes B cell proliferation, differentiation, immunoglobulin isotype switching, germinal center formation, and stimulation of the humoral memory response. CD40 has been found to mediate a broad variety of immune and inflammatory responses. Within the cell, the CD40 Molecule acts as a transmembrane signal transducer that leads to activation of intracellular kinases and transcription factors.
UniProt P25942
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN2451933) Images back to top
Application Notes 1. Flowcytometry
2. Immunohistochemistry (acetone or zinc-fixed or frozen section, indirect immuno-staining)
3. Stimulation of proliferation of B cell and dendritic cells. Enhancement of proinflamentory cytokine production in human monocytes,such as TNF-a,IL-6 and IL-8
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN2451933) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol. Azide- and carrier protein-free.
Preservative Azide free
Storage -20 °C

References for anti-CD40 antibody (ABIN2451933)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Product cited in:

Ishida, Kumanogoh, Suzuki, Akahani, Noda, Kikutani: "Involvement of CD100, a lymphocyte semaphorin, in the activation of the human immune system via CD72: implications for the regulation of immune and inflammatory responses." in: International immunology, Vol. 15, Issue 8, pp. 1027-34, 2003

Yasui, Muraoka, Takaoka-Shichijo, Ishida, Takegahara, Uchida, Kumanogoh, Suematsu, Suzuki, Kikutani: "Dissection of B cell differentiation during primary immune responses in mice with altered CD40 signals." in: International immunology, Vol. 14, Issue 3, pp. 319-29, 2002

Inui, Kaisho, Kikutani, Stamenkovic, Seed, Clark, Kishimoto: "Identification of the intracytoplasmic region essential for signal transduction through a B cell activation molecule, CD40." in: European journal of immunology, Vol. 20, Issue 8, pp. 1747-53, 1990

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN2451933) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (Extracellular Domain) antibody (ABIN2451933) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (Extracellular Domain) antibody
Immunofluorescence (IF) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (Extracellular Domain) antibody (ABIN2451933) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (Extracellular Domain) antibody (Image 2)
Western Blotting (WB) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (Extracellular Domain) antibody (ABIN2451933) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (Extracellular Domain) antibody (Image 3)