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anti-Human CD40 antibody for Western Blotting

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Epitope
N-Term
54
37
36
25
24
22
19
18
11
8
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rabbit
625
307
69
20
14
10
9
6
4
2
2
2
1
1
1
Host
Rabbit
492
208
158
23
6
4
Clonality
Polyclonal
Conjugate
This CD40 antibody is un-conjugated
87
84
49
24
22
21
17
15
13
13
12
9
9
9
9
9
9
9
8
7
7
7
7
6
6
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
560
233
200
152
106
103
92
62
45
33
19
15
13
9
8
6
6
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN2777069
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1870732 ABIN222919 WB Rabbit IgG Log in to see Polyclonal 1
3.1870732 ABIN1531702 ELISA WB Rabbit IgG pThr254 Log in to see Polyclonal 1
3.1870732 ABIN2777068 WB Rabbit N-Term Log in to see Polyclonal
3.1870732 ABIN968494 IF WB Mouse IgG1 AA 71-187 Log in to see 41-CD40 4
3.1870732 ABIN968495 IF WB Mouse IgG1 AA 71-187 Log in to see 41-CD40 4
3.1870732 ABIN4293058 ELISA WB Goat Isoform 1 Log in to see Polyclonal 1
3.1870732 ABIN1536939 WB Rabbit Ig AA 247-276, C-Term Log in to see Polyclonal
3.1870732 ABIN1539964 WB Mouse IgG1 AA 247-276, C-Term Log in to see 647CT13-2-4
3.1870732 ABIN1533424 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.1870732 ABIN3183772 ELISA IHC WB Rabbit IgG C-Term Log in to see Polyclonal
3.1870732 ABIN3182275 ELISA WB Rabbit IgG pThr254 Log in to see Polyclonal
3.1870732 ABIN2704615 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
3.1870732 ABIN1106352 WB Rabbit IgG N-Term Log in to see Polyclonal
3.1870732 ABIN2462500 ELISA WB Rabbit Log in to see Polyclonal
3.1870732 ABIN2458616 ELISA WB Rabbit Log in to see Polyclonal
3.1870732 ABIN2855046 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.1870732 ABIN1871634 WB Rabbit IgG Log in to see Polyclonal
3.1870732 ABIN3020704 WB Rabbit IgG C-Term Log in to see Polyclonal
3.1870732 ABIN1997461 ELISA WB Rabbit IgG AA 1-193, Extracellular Domain Log in to see Polyclonal
3.1870732 ABIN1584207 ELISA WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-CD40 antibody for Western Blotting

Similar anti-CD40 Antibodies

Application / Reactivity Human Rabbit
Immunofluorescence (fixed cells) (IF/ICC) 14 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
Western Blotting (WB) 219 Antibodies 3 Antibodies
Stimulation (St) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 8 Antibodies
Indirect Immunofluorescence Assay (IFA) 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 51 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 105 Antibodies
Immunohistochemistry (IHC) 119 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 65 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunofluorescence (IF) 56 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope N-Term
(54), (37), (36), (25), (24), (22), (19), (18), (11), (8), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rabbit
(625), (307), (69), (20), (14), (10), (9), (6), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(492), (208), (158), (23), (6), (4)
Clonality
Polyclonal
Conjugate This CD40 antibody is un-conjugated
(87), (84), (49), (24), (22), (21), (17), (15), (13), (13), (12), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(560), (233), (200), (152), (106), (103), (92), (62), (45), (33), (19), (15), (13), (9), (8), (6), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN2777069) Images
Blocking Peptide Blocking peptide for this product available: ABIN974676
Sequence WNRETHCHQH KYCDPNLGLR VQQKGTSETD TICTCEEGWH CTSEACESCV
Predicted Reactivity Human: 100%, Rabbit: 79%
Characteristics This is a rabbit polyclonal antibody against CD40. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CD40

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN2777069) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Alias Symbols: Bp50, CDW40, MGC9013, TNFRSF5, p50
Protein Interaction Partner: TRAF2, TRAF3, PIK3R1, CD40LG, BAG3, Otud7b, Birc3, IL4R, TRAF6, TRAF5, TRAF3IP2, MAP3K8, TRAF4, TRAF1, PIK3CA, CBL, BIRC2, CDC40, TANK, HSPA4, NSMAF, XRCC5, HSPA8, TDP2, RIPK2, CAV1, JAK3, XRCC6, MS4A1, SUMO1, SLC30A7, RNF219, UGGT1, ERP44, NCOA6, USP15,
Protein Size: 277
Molecular Weight 30 kDa
Gene ID 958
NCBI Accession NM_001250, NP_001241
UniProt P25942
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN2777069) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 277 AA

Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN2777069) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CD40 Antibody (ABIN2777069)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

von Bergwelt-Baildon, Shimabukuro-Vornhagen, Popov, Klein-Gonzalez, Fiore, Debey, Draube, Maecker, Menezes, Nadler, Schultze: "CD40-activated B cells express full lymph node homing triad and induce T-cell chemotaxis: potential as cellular adjuvants." in: Blood, Vol. 107, Issue 7, pp. 2786-9, 2006

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN2777069) back to top
Supplier Images
Western Blotting (WB) image for anti-CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5) (N-Term) (ABIN2777069) WB Suggested Anti-CD40 Antibody Titration: 0.2-1 ug/ml
Positive Control:...