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anti-Mouse (Murine) CD40 antibody for Enzyme Immunoassay

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Epitope
Extracellular Domain
61
39
36
28
22
19
15
10
9
7
5
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
633
303
55
13
11
9
8
5
4
2
1
1
1
1
1
Host
Rat
502
213
160
24
4
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
95
92
53
21
21
18
15
13
13
12
12
9
9
9
9
9
9
9
8
7
6
6
6
4
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
572
223
198
149
102
97
95
71
43
42
19
13
9
8
8
6
5
4
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN118121
$ 621.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN118122 EIA FACS IHC (fro) Rat IgG2a Extracellular Domain Log in to see 01-03-23 3
1 ABIN118123 EIA FACS IHC (fro) Rat IgG2a Extracellular Domain Log in to see 01-03-23 2
1 ABIN319540 EIA FACS IHC (fro) Rat IgG2a Extracellular Domain Log in to see 01-03-23 3

Top referenced anti-CD40 antibody for Enzyme Immunoassay

Similar anti-CD40 Antibodies

Application / Reactivity Mouse (Murine)
Activity Assay (AcA) 1 Antibodies
Agonist (Agon) 2 Antibodies
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
ELISA 48 Antibodies
ELISA (Capture) 9 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 214 Antibodies
Functional Studies (Func) 20 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 34 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope Extracellular Domain
(61), (39), (36), (28), (22), (19), (15), (10), (9), (7), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(633), (303), (55), (13), (11), (9), (8), (5), (4), (2), (1), (1), (1), (1), (1)
Host Rat
(502), (213), (160), (24), (4), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(95), (92), (53), (21), (21), (18), (15), (13), (13), (12), (12), (9), (9), (9), (9), (9), (9), (9), (8), (7), (6), (6), (6), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(572), (223), (198), (149), (102), (97), (95), (71), (43), (42), (19), (13), (9), (8), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN118121) Images
Specificity Specificity was checked by ELISA and flow cytometry using BHK cells transfected with mouse CD40. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. Clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
Purification Affinity Chromatography on Protein G.
Endotoxin Level Low Endotoxin
Immunogen Extracellular domain of Mouse CD40 and the Fc portion of Human IgG1. Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.
Clone 01-03-23
Isotype IgG2a

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN118121) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5
Gene ID 21939
NCBI Accession NP_035741
UniProt P27512
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN118121) Images back to top
Application Notes Flow Cytometry: use 10 μL of 1/50 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. ELISA. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN118121) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-CD40 Antibody (ABIN118121)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Hasbold, Klaus: "B cells from CBA/N mice do not proliferate following ligation of CD40." in: European journal of immunology, Vol. 24, Issue 1, pp. 152-7, 1994

Hasbold, Johnson-Léger, Atkins, Clark, Klaus: "Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies." in: European journal of immunology, Vol. 24, Issue 8, pp. 1835-42, 1994

Parry, Holman, Hasbold, Klaus: "Plastic-immobilized anti-mu or anti-delta antibodies induce apoptosis in mature murine B lymphocytes." in: European journal of immunology, Vol. 24, Issue 4, pp. 974-9, 1994

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN118121) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5) (Extracellular Domain) (ABIN118121) Staining of mouse spleen with RAT ANTI MOUSE CD40: LOW ENDOTOXIN