anti-Mouse (Murine) CD40 antibody for ELISA (Capture)

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Epitope
AA 24-193
55
52
47
26
24
22
19
18
18
8
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
659
330
69
20
14
10
10
6
4
3
2
2
1
1
1
1
Host
Rat
515
230
171
23
6
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
87
86
50
27
25
24
20
18
14
13
12
10
10
10
9
9
9
9
9
9
9
8
7
6
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Western Blotting (WB)
608
246
207
157
112
103
103
65
47
36
19
17
15
13
9
8
6
6
3
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN4899798
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2662062 ELISA (Capture) FITC Hamster IgM kappa, IgM Log in to see HM40-3 11
1 ABIN2661078 ELISA (Capture) WB Biotin Rat IgG2a kappa Log in to see 3-23 4
1 ABIN2661714 ELISA (Capture) ELISPOT (Capture) FITC Rat IgG2a kappa Log in to see 3-23 3
1 ABIN2659266 ELISA (Capture) PE-Cy7 Rat IgG2a kappa Log in to see 3-23 3
1 ABIN5646237 CyTOF ELISA (Capture) FACS WB Rat IgG1 AA 24-193 Log in to see 65924
1 ABIN2662061 ELISA (Capture) FITC Hamster IgM kappa, IgM Log in to see HM40-3 11
1 ABIN2661077 ELISA (Capture) WB Biotin Rat IgG2a kappa Log in to see 3-23 4
1 ABIN2661713 ELISA (Capture) ELISPOT (Capture) FITC Rat IgG2a kappa Log in to see 3-23 3
1 ABIN2659267 ELISA (Capture) PE-Cy7 Rat IgG2a kappa Log in to see 3-23 3

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope AA 24-193
(55), (52), (47), (26), (24), (22), (19), (18), (18), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(659), (330), (69), (20), (14), (10), (10), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rat
(515), (230), (171), (23), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(87), (86), (50), (27), (25), (24), (20), (18), (14), (13), (12), (10), (10), (10), (9), (9), (9), (9), (9), (9), (9), (8), (7), (6), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Western Blotting (WB)
(608), (246), (207), (157), (112), (103), (103), (65), (47), (36), (19), (17), (15), (13), (9), (8), (6), (6), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN4899798) Images
Specificity Detects mouse CD40/TNFRSF5 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CD40/TNFRSF5, recombinant mouse (rm) CD40 Ligand, rmCD27/TNFRSF7, rmCD30/TNFRSF8, or rmFas/TNFRSF6 is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse CD40/TNFRSF5, Val24-Arg193, Accession # P27512
Clone 65924
Isotype IgG1

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN4899798) Images back to top
Antigen
Alternative Name CD40/TNFRSF5 (CD40 Antibody Abstract)
Background Alternative Names: B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40, CD40 antigen, CD40 Molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, nerve growth factor receptor-related B-lymphocyte activation molecule, p50, TNFRSF5, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5
Gene ID 958
UniProt P27512
Research Area Stem Cells, Adaptive Immunity, Hematopoietic Progenitors, Surface Receptors of Immune Cells, CD Antigens, Inflammation, Apoptosis/Necrosis, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN4899798) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 2-8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN4899798) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD40 antibody (ABIN4899798)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Product cited in:

Zhang, Yin, Zhang, Sun, Yang, Zhang, Salzman, Wang, Liu, Vidyasagar, Zhang, Ju, Okunieff, Zhang: "Response patterns of cytokines/chemokines in two murine strains after irradiation." in: Cytokine, Vol. 58, Issue 2, pp. 169-77, 2012 Method employed by authors: Luminex Assay (LMNX) (Sample species: Mouse (Murine)).