anti-Mouse (Murine) CD40 antibody for Flow Cytometry

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Epitope
Extracellular Domain
55
52
46
26
24
22
19
19
18
8
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
659
330
69
20
14
10
10
6
4
3
2
2
1
1
1
1
Host
Rat
515
230
171
23
6
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is conjugated to FITC
86
86
50
27
25
24
20
18
14
13
12
10
10
10
9
9
9
9
9
9
9
8
7
6
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
608
247
207
157
112
103
103
65
47
36
19
18
15
13
10
8
6
6
3
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN118120
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.996978 ABIN118124 FACS PE Rat IgG2a Extracellular Domain Log in to see 01-03-23 3
13.996978 ABIN118121 EIA Func FACS IHC (fro) Rat IgG2a Extracellular Domain Log in to see 01-03-23 3
13.996978 ABIN370939 FACS FITC Rat IgG2a Log in to see 1C10 6
13.996978 ABIN262357 ICC FACS IHC (fro) IF IHC Armenian Hamster IgM Log in to see HM40-3 3
13.996978 ABIN439951 AcA BP FACS IP in vitro Neut Rat IgG2a kappa Log in to see 1C10 1
13.996978 ABIN2656794 FACS Alexa Fluor 488 Hamster IgM kappa, IgM Log in to see HM40-3 11
13.996978 ABIN2480318 IHC (fro) FACS ELISA Rat IgG2a Extracellular Domain Log in to see 3-23 4
13.996978 ABIN2657787 FACS IF IHC Alexa Fluor 647 Hamster IgM kappa, IgM Log in to see HM40-3 11
13.996978 ABIN2663454 ELISA (Detection) FACS PE Rat IgG2a kappa Log in to see 3-23 5
13.996978 ABIN2658979 BR FACS IP IHC PE-Cy5 Rat IgG2a kappa Log in to see 3-23 3
13.996978 ABIN2665699 FACS Rat IgG2a kappa Log in to see 1C10 9
13.996978 ABIN2665994 FACS Rat IgG2a kappa Log in to see 1C10 9
13.996978 ABIN254885 FACS PE Rat IgG2a Log in to see 39530
13.996978 ABIN2664677 FACS Rat IgG2a kappa Log in to see 3-23 3
13.996978 ABIN2664678 FACS Rat IgG2a kappa Log in to see 1C10 9
13.996978 ABIN2660522 FACS Hamster IgM kappa, IgM Log in to see HM40-3 11
13.996978 ABIN439954 FACS in vitro IP PE Rat IgG2a kappa Log in to see 1C10
13.996978 ABIN4897889 FACS Fluorescein Rat IgG2a Extracellular Domain Log in to see 1C10
13.996978 ABIN262359 FACS FITC Armenian Hamster IgM Log in to see HM40-3
13.996978 ABIN254884 FACS FITC Rat IgG2a Log in to see 39530

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope Extracellular Domain
(55), (52), (46), (26), (24), (22), (19), (19), (18), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(659), (330), (69), (20), (14), (10), (10), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rat
(515), (230), (171), (23), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is conjugated to FITC
(86), (86), (50), (27), (25), (24), (20), (18), (14), (13), (12), (10), (10), (10), (9), (9), (9), (9), (9), (9), (9), (8), (7), (6), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(608), (247), (207), (157), (112), (103), (103), (65), (47), (36), (19), (18), (15), (13), (10), (8), (6), (6), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN118120) Images
Specificity Specificity was checked by ELISA and flow cytometry using BHK cells transfected with mouse CD40. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. Clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
Purification Affinity Chromatography on Protein G
Immunogen Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1. Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.
Clone 01-03-23
Isotype IgG2a

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN118120) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5
Gene ID 21939
NCBI Accession NP_035741
UniProt P27512
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN118120) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing lowaffinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN118120) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD40 antibody (ABIN118120)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Hasbold, Johnson-Léger, Atkins, Clark, Klaus: "Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies." in: European journal of immunology, Vol. 24, Issue 8, pp. 1835-42, 1994

Parry, Holman, Hasbold, Klaus: "Plastic-immobilized anti-mu or anti-delta antibodies induce apoptosis in mature murine B lymphocytes." in: European journal of immunology, Vol. 24, Issue 4, pp. 974-9, 1994

Hasbold, Klaus: "B cells from CBA/N mice do not proliferate following ligation of CD40." in: European journal of immunology, Vol. 24, Issue 1, pp. 152-7, 1994

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN118120) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (Extracellular Domain) antibody (FITC) (ABIN118120) Staining of mouse spleen cells with RAT ANTI MOUSE CD40:FITC