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|Antigen||CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD40 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
|3 references available|
|Supplier||Log in to see|
Product Details anti-CD40 AntibodyTarget Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN118121) Images
|Specificity||Specificity was checked by ELISA and flow cytometry using BHK cells transfected with mouse CD40. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. Clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.|
|Purification||Affinity Chromatography on Protein G.|
|Endotoxin Level||Low Endotoxin|
|Immunogen||Extracellular domain of Mouse CD40 and the Fc portion of Human IgG1. Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.|
Target Details CD40Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN118121) Images back to top
|Alternative Name||CD40 (CD40 Antibody Abstract)|
|Background||CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5|
|Research Area||Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer|
|Pathways||NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN118121) Images back to top
Flow Cytometry: use 10 μL of 1/50 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. ELISA. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN118121) Images back to top
|Buffer||PBS, pH 7.4 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|
References for anti-CD40 antibody (ABIN118121)Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Hasbold, Klaus: "B cells from CBA/N mice do not proliferate following ligation of CD40." in: European journal of immunology, Vol. 24, Issue 1, pp. 152-7, 1994
Hasbold, Johnson-Léger, Atkins, Clark, Klaus: "Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies." in: European journal of immunology, Vol. 24, Issue 8, pp. 1835-42, 1994
Parry, Holman, Hasbold, Klaus: "Plastic-immobilized anti-mu or anti-delta antibodies induce apoptosis in mature murine B lymphocytes." in: European journal of immunology, Vol. 24, Issue 4, pp. 974-9, 1994
ImagesProduct Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN118121) back to top