|Application / Reactivity||Mouse (Murine)|
|Blocking Reagent (BR)||3 Antibodies|
|ELISA (Capture)||8 Antibodies|
|ELISA (Detection)||2 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||2 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||186 Antibodies|
|Functional Studies (Func)||23 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||8 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||27 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||19 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||10 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||1 Antibodies|
|Immunoprecipitation (IP)||40 Antibodies|
|Antigen||CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD40 antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))
|5 references available|
|Supplier||Log in to see|
Product Details anti-CD40 AntibodyTarget Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN2689246) Images
The 3/23 clone reacts with CD40, a 40-50 kDa glycoprotein expressed on B lymphocytes and other antigen-presenting cells. CD40 has been reported to be transiently expressed on activated CD4+ and CD8+ T cells and in some mouse strains, the 3/23 mAb has been reported to react with 5-10 % of T lymphocytes in adult mouse, but not neonatal, spleen. CD40 plays a key role in B-cell growth and differentiation where interactions of CD40 with its ligand, CD154, are involved in the initiation, effector, and memory stages of cell-mediated immune responses. In addition, CD40 has been reported to be involved with the triggering of NK cells and NK-T cells. Ligation of CD40 with the 3/23 antibody has been reported to induce splenic B cells to express the costimulatory molecule CD86 (B7-2). In addition, although the 3/23 antibody by itself is a weak B-cell mitogen, it has been reported to synergize markedly with mitogenic anti-IgM, anti-IgD mAb or IL-4 to promote B-cell proliferation. Immunohistochemical staining of CD40. Frozen sections of normal mouse thymus was reacted with the 3/23 antibody. B lymphocytes and dendritic cells can be identified by the brown labeling of their cell surface membranes. Amplification 20x.
BD Pharmingen™ Purified Rat Anti-Mouse CD40 - Purified - Clone 3/23 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Mouse CD40 Recombinant Protein|
Target Details CD40Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN2689246) Images back to top
|Alternative Name||CD40 (CD40 Antibody Abstract)|
|Research Area||Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer|
Application DetailsProduct Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN2689246) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN2689246) Images back to top
|Buffer||Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-CD40 Antibody (ABIN2689246)Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
|Product cited in:||
Klaus, Holman, Hasbold: "Properties of mouse CD40: the role of homotypic adhesion in the activation of B cells via CD40." in: European journal of immunology, Vol. 24, Issue 11, pp. 2714-9, 1994 (PubMed).
Hasbold, Johnson-Léger, Atkins et al.: "Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies." in: European journal of immunology, Vol. 24, Issue 8, pp. 1835-42, 1994 (PubMed).
Hasbold, Klaus: "B cells from CBA/N mice do not proliferate following ligation of CD40." in: European journal of immunology, Vol. 24, Issue 1, pp. 152-7, 1994 (PubMed).
Parry, Hasbold, Holman et al.: "Hypercross-linking surface IgM or IgD receptors on mature B cells induces apoptosis that is reversed by costimulation with IL-4 and anti-CD40." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2821-9, 1994 (PubMed).
Noelle, Ledbetter, Aruffo: "CD40 and its ligand, an essential ligand-receptor pair for thymus-dependent B-cell activation." in: Immunology today, Vol. 13, Issue 11, pp. 431-3, 1993 (PubMed).