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anti-Rabbit CD40 antibody for Western Blotting

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Epitope
AA 35-84
53
37
36
25
24
22
19
17
11
8
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Pig (Porcine), Rabbit
623
306
69
20
13
10
9
6
3
2
2
1
1
Host
Rabbit
493
208
156
23
4
4
Clonality
Polyclonal
Conjugate
This CD40 antibody is un-conjugated
88
84
49
24
22
21
17
15
13
13
12
9
9
9
9
9
9
9
8
7
7
7
7
6
6
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
561
229
200
152
106
103
93
62
46
33
19
15
13
9
8
6
6
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN463276
$ 551.83
Plus shipping costs $45.00

Similar anti-CD40 Antibodies

Application / Reactivity Human Pig (Porcine) Monkey Rabbit
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Cytometry by Time of Flight (CyTOF) 8 Antibodies
Western Blotting (WB) 216 Antibodies 10 Antibodies 11 Antibodies 1 Antibodies
Stimulation (St) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 8 Antibodies
Indirect Immunofluorescence Assay (IFA) 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 52 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 105 Antibodies 3 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 119 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope AA 35-84
(53), (37), (36), (25), (24), (22), (19), (17), (11), (8), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Pig (Porcine), Rabbit
(623), (306), (69), (20), (13), (10), (9), (6), (3), (2), (2), (1), (1)
Host Rabbit
(493), (208), (156), (23), (4), (4)
Clonality
Polyclonal
Conjugate This CD40 antibody is un-conjugated
(88), (84), (49), (24), (22), (21), (17), (15), (13), (13), (12), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(561), (229), (200), (152), (106), (103), (93), (62), (46), (33), (19), (15), (13), (9), (8), (6), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling Images
Specificity Human CD40
Predicted Reactivity Percent identity by BLAST analysis: Human (100%) Guinea pig (90%) Pig (83%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide located between aa35-84 of human CD40 (P25942, NP_690593). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Rabbit (100%), Galago, Guinea pig (90%), Horse, Pig (83%).

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background Name/Gene ID: CD40
Family: TNF Receptor

Synonyms: CD40, B-cell surface antigen CD40, B cell-associated molecule, B cell surface antigen CD40, CD40 antigen, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TNFRSF5
Gene ID 958
NCBI Accession NP_690593
UniProt P25942
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling Images back to top
Application Notes Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Not recommended for: IHC-P
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details Images back to top
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling back to top
Supplier Images
 image for anti-CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5) (AA 35-84) (ABIN463276) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 35-84) antibody