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anti-Rat (Rattus) CD40 antibody for Flow Cytometry

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • 2310042E05Rik
  • AI326936
  • Bp50
  • C87049
  • C130077D06Rik
  • CDW40
  • DCTN-50
  • GMP23-48K
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • p50
  • RBP50
  • T-BAM
  • Tnfrsf5
  • TNFRSF5
  • TNFSF5
  • TRAP
Reactivity
Mouse (Murine), Rat (Rattus)
605
301
56
13
11
8
7
5
4
2
1
1
1
1
1
Host
Armenian Hamster
481
211
154
24
8
2
Clonality
Monoclonal
Conjugate
This CD40 antibody is conjugated to EFluor 450
95
91
52
24
20
17
14
12
12
11
9
8
8
8
8
8
8
8
8
8
7
6
6
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
565
200
199
107
87
78
71
49
48
22
19
11
8
8
6
3
3
3
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN2678915
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.301884 ABIN2678913 FACS APC Armenian Hamster IgM kappa Log in to see HM40-3 1
0.0010452035 ABIN282199 Func FACS Armenian Hamster IgM kappa Log in to see HM40-3 1
0.0010452035 ABIN280350 Func FACS IHC Armenian Hamster IgM kappa Log in to see HM40-3 1
0.0010452035 ABIN1176841 BR FACS Armenian Hamster IgM, kappa Log in to see HM40-3 15
0.0010452035 ABIN277764 FACS FITC Armenian Hamster IgM kappa Log in to see HM40-3 1
0.0010452035 ABIN2689247 FACS Armenian Hamster IgM, kappa Log in to see HM40 15
0.0010452035 ABIN2567691 FACS FITC Hamster IgM kappa Log in to see HM40-3
0.0010452035 ABIN2567690 FACS FITC Hamster IgM kappa Log in to see HM40-3
0.0010452035 ABIN2567692 FACS FITC Hamster IgM kappa Log in to see HM40-3
0.0010452035 ABIN2567693 FACS FITC Hamster IgM kappa Log in to see HM40-3
0.0010452035 ABIN2567688 Func FACS Hamster IgM kappa Log in to see HM40-3
0.0010452035 ABIN2567687 Func FACS Hamster IgM kappa Log in to see HM40-3
0.0010452035 ABIN2567686 Func FACS Hamster IgM kappa Log in to see HM40-3
0.0010452035 ABIN2567689 Func FACS Hamster IgM kappa Log in to see HM40-3
0.0010452035 ABIN2136577 Func FACS IP IHC Hamster IgM kappa Log in to see HM40-3
0.0010452035 ABIN2691649 FACS FITC Mouse Log in to see
0.0010452035 ABIN2691651 FACS PE Mouse Log in to see

Top referenced anti-CD40 antibody for Flow Cytometry

Similar anti-CD40 Antibodies

Application / Reactivity Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 43 Antibodies 23 Antibodies
Neutralization (Neut) 3 Antibodies
Intracellular Flow Cytometry (ICFC) 11 Antibodies
In vitro Assay (in vitro) 6 Antibodies
Immunoprecipitation (IP) 47 Antibodies 8 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies 10 Antibodies
Immunohistochemistry (IHC) 29 Antibodies 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 38 Antibodies 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunocytochemistry (ICC) 3 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(605), (301), (56), (13), (11), (8), (7), (5), (4), (2), (1), (1), (1), (1), (1)
Host Armenian Hamster
(481), (211), (154), (24), (8), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is conjugated to EFluor 450
(95), (91), (52), (24), (20), (17), (14), (12), (12), (11), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (6), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(565), (200), (199), (107), (87), (78), (71), (49), (48), (22), (19), (11), (8), (8), (6), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN2678915) Images
Specificity The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Characteristics The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Synonyms: Synonyms: TNFRSF5
Clone HM40-3
Isotype IgM kappa

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN2678915) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN2678915) Images back to top
Application Notes Applications reported: This HM40-3 antibody has been reported for use in flow cytometric analysis.

Applications tested: This HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is a replacement for Pacific Blue®. eFluor® 450 emits at 456 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN2678915) Images back to top
Concentration 0.2 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

References for anti-CD40 Antibody (ABIN2678915)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Kawano, Cui, Koezuka, Toura, Kaneko, Motoki, Ueno, Nakagawa, Sato, Kondo, Koseki, Taniguchi: "CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1626-9, 1997

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN2678915) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5)  (EFluor 450) (ABIN2678915) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) antibody (EFluor 450)