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anti-Mouse (Murine) CD40 Ligand antibody for Flow Cytometry

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD154
  • cd40l
  • CD40L
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TNFSF5
  • TRAP
  • gp39
  • hCD40L
  • CD40-L
  • Cd40l
  • Ly-62
  • Ly62
  • Tnfsf5
  • CD40 ligand
  • TNF superfamily member 5
  • CD40LG
  • cd40l
  • Cd40lg
  • LOC100358388
  • CD154
Epitope
AA 61-260
21
16
15
15
14
12
9
9
7
7
6
6
5
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
455
233
55
20
20
10
8
8
5
5
4
3
3
3
3
2
2
1
Host
Rat
276
167
106
51
34
26
1
Clonality
Monoclonal
Conjugate
This CD40 Ligand antibody is un-conjugated
73
58
43
14
14
12
12
11
9
8
8
8
8
8
8
6
6
6
6
5
5
5
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Neutralization (Neut)
400
271
170
93
66
51
49
42
33
20
15
13
11
9
7
7
6
5
4
4
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN4900437
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.2075615 ABIN4897698 FACS PE Rat IgG2a AA 61-260 Log in to see 208109 1
14.2075615 ABIN1176849 BR FACS IHC Armenian Hamster IgG3, kappa Log in to see MR1 23
14.2075615 ABIN674439 FACS IF (p) IHC (p) Rabbit IgG AA 47-80 Log in to see Polyclonal 1
14.2075615 ABIN370946 FACS FITC Hamster IgG Log in to see MR1 7
14.2075615 ABIN2659110 FACS PE-Cy7 Hamster IgG Log in to see MR1 8
14.2075615 ABIN2663050 FACS PE Hamster IgG Log in to see MR1 10
14.2075615 ABIN2660955 FACS IF IHC Biotin Hamster IgG Log in to see MR1 8
14.2075615 ABIN2659972 FACS PerCP-Cy5.5 Hamster IgG Log in to see MR1 8
14.2075615 ABIN2656942 FACS WB APC Hamster IgG Log in to see MR1 9
14.2075615 ABIN2665556 BR FACS IHC Hamster IgG Log in to see MR1 8
14.2075615 ABIN4292990 FACS Alexa Fluor 594 Rat IgG2a AA 61-260 Log in to see 208109
14.2075615 ABIN4897696 FACS Allophycocyanin Rat IgG2a AA 61-260 Log in to see 208109
14.2075615 ABIN4897697 FACS Fluorescein Rat IgG2a AA 61-260 Log in to see 208109
14.2075615 ABIN439957 FACS IHC (fro) IP in vitro FITC Armenian Hamster IgG1 kappa Log in to see MR1
1 ABIN118278 FACS FITC Hamster IgG Log in to see 39H5 2
1 ABIN674446 FACS IF (p) FITC Rabbit IgG AA 47-80 Log in to see Polyclonal 1
1 ABIN1302520 Func ICC FACS IP Hamster IgG1 Log in to see MR-1 8
1 ABIN2688995 FACS Biotin Armenian Hamster IgG3, kappa Log in to see MR1 22
1 ABIN370945 FACS Biotin Hamster IgG Log in to see MR1 7
1 ABIN1302448 Func ICC FACS IP Hamster IgG1 Log in to see MR-1 7

Top referenced anti-CD40 Ligand antibody for Flow Cytometry

Similar anti-CD40 Ligand Antibodies

Application / Reactivity Mouse (Murine)
Blocking Peptide (BP) 5 Antibodies
Blocking Reagent (BR) 5 Antibodies
Cytometry by Time of Flight (CyTOF) 5 Antibodies
ELISA 9 Antibodies
Flow Cytometry (FACS) 185 Antibodies
Functional Studies (Func) 16 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies
Immunoprecipitation (IP) 52 Antibodies
In vitro Assay (in vitro) 1 Antibodies
In vivo Studies (in vivo) 2 Antibodies
Neutralization (Neut) 10 Antibodies
Western Blotting (WB) 38 Antibodies

General

Antigen CD40 Ligand (CD40LG) Antibodies
Epitope AA 61-260
(21), (16), (15), (15), (14), (12), (9), (9), (7), (7), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(455), (233), (55), (20), (20), (10), (8), (8), (5), (5), (4), (3), (3), (3), (3), (2), (2), (1)
Host Rat
(276), (167), (106), (51), (34), (26), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 Ligand antibody is un-conjugated
(73), (58), (43), (14), (14), (12), (12), (11), (9), (8), (8), (8), (8), (8), (8), (6), (6), (6), (6), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Neutralization (Neut)
(400), (271), (170), (93), (66), (51), (49), (42), (33), (20), (15), (13), (11), (9), (7), (7), (6), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN4900437) Images
Specificity Detects mouse CD40 Ligand in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) CD27 Ligand, rmCD30 Ligand, or recombinant human CD40 Ligand is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse CD40 Ligand/TNFSF5, Glu61-Leu260, Accession # P27548
Clone 208109
Isotype IgG2a

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN4900437) Images back to top
Antigen
Alternative Name CD40 Ligand/TNFSF5 (CD40LG Antibody Abstract)
Background Alternative Names: CD154, CD154 antigen, CD40 antigen ligand, CD40 ligand, CD40-L, CD40LG, CD40LIGM, gp39, hCD40L, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5
Gene ID 959
UniProt P27548
Pathways NF-kappaB Signaling, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN4900437) Images back to top
Application Notes Flow Cytometry 0.25 μg/10^6 cells
Neutralization 0.0075-0.045 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN4900437) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD40 Ligand Antibody (ABIN4900437)

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Product cited in:

Ito, Ando, Ishikawa, Nakayama, Taniguchi, Saito, Imawari, Moriwaki, Yokochi, Kakumu, Seishima: "Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance." in: International immunology, Vol. 20, Issue 7, pp. 869-79, 2008 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN4900437) back to top
Supplier Images
Neutralization (Neut) image for anti-CD40 Ligand antibody (CD40LG) (AA 61-260) (ABIN4900437) Proliferation Induced by CD40 Ligand/TNFSF5 and Neutralization by Mouse CD40 Ligand/T...
Flow Cytometry (FACS) image for anti-CD40 Ligand antibody (CD40LG) (AA 61-260) (ABIN4900437) Detection of CD40 Ligand/TNFSF5 in Mouse Splenocytes by Flow Cytometry. Mouse splenoc...