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anti-Mouse (Murine) CD40 Ligand antibody for Flow Cytometry

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD40-L
  • Cd40l
  • CD40L
  • cd40l
  • CD154
  • gp39
  • hCD40L
  • HIGM1
  • IGM
  • IMD3
  • Ly-62
  • Ly62
  • T-BAM
  • Tnfsf5
  • TNFSF5
  • TRAP
Reactivity
Mouse (Murine)
451
209
40
15
14
11
7
4
3
3
3
2
2
2
2
Host
Armenian Hamster
281
146
103
55
28
26
Clonality
Monoclonal
Conjugate
This CD40 Ligand antibody is un-conjugated
78
60
43
11
11
9
9
8
8
8
7
7
7
7
7
7
7
6
6
6
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Immunohistochemistry (IHC)
373
250
171
59
57
54
52
50
41
22
16
15
11
7
6
5
5
4
3
3
3
2
2
1
1
1
1
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Catalog No. ABIN1176849
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30379575 ABIN370946 FACS FITC Hamster IgG Log in to see MR1 7
0.30379575 ABIN2659110 FACS PE-Cy7 Hamster IgG Log in to see MR1 8
0.30379575 ABIN2660955 FACS IF IHC Biotin Hamster IgG Log in to see MR1 8
0.30379575 ABIN2656942 FACS WB APC Hamster IgG Log in to see MR1 9
0.30379575 ABIN2659972 FACS PerCP-Cy5.5 Hamster IgG Log in to see MR1 8
0.30379575 ABIN2663050 FACS PE Hamster IgG Log in to see MR1 10
0.30379575 ABIN2665556 BR FACS IHC Hamster IgG Log in to see MR1 8
0.30379575 ABIN439957 FACS in vitro IP FITC Armenian Hamster IgG1 kappa Log in to see MR1
0.30379575 ABIN2677636 FACS EFluor 450 Armenian Hamster IgG Log in to see MR1
0.30379575 ABIN2677634 FACS PerCP-eFluor® 710 Armenian Hamster IgG Log in to see MR1
0.30379575 ABIN2677632 FACS PE-Cy7 Armenian Hamster IgG Log in to see MR1
0.30379575 ABIN2168632 FACS FITC Hamster IgG Log in to see Polyclonal
0.30379575 ABIN2168631 FACS Biotin Hamster IgG Log in to see Polyclonal
0.30379575 ABIN2168634 FACS PE Hamster IgG Log in to see Polyclonal
0.30379575 ABIN2168633 FACS Hamster IgG Log in to see Polyclonal
0.30379575 ABIN2168635 FACS Hamster IgG Log in to see Polyclonal
0.0010485112 ABIN254601 FACS PE Armenian Hamster IgG Log in to see 39H5
0.0010485112 ABIN118278 FACS FITC Hamster IgG Log in to see 39H5 2
0.0010485112 ABIN254600 FACS FITC Armenian Hamster IgG Log in to see 39H5
0.0010485112 ABIN288650 FACS PE Hamster IgG Log in to see MR1

Top referenced anti-CD40 Ligand antibody for Flow Cytometry

Similar anti-CD40 Ligand Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 3 Antibodies
ELISA 8 Antibodies
Flow Cytometry (FACS) 167 Antibodies
Functional Studies (Func) 21 Antibodies
Immunocytochemistry (ICC) 34 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 23 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 48 Antibodies
In vitro Assay (in vitro) 3 Antibodies
In vivo Studies (in vivo) 3 Antibodies
Neutralization (Neut) 16 Antibodies
Western Blotting (WB) 32 Antibodies

General

Antigen CD40 Ligand (CD40LG) Antibodies
Reactivity Mouse (Murine)
(451), (209), (40), (15), (14), (11), (7), (4), (3), (3), (3), (2), (2), (2), (2)
Host Armenian Hamster
(281), (146), (103), (55), (28), (26)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 Ligand antibody is un-conjugated
(78), (60), (43), (11), (11), (9), (9), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS), Immunohistochemistry (IHC)
(373), (250), (171), (59), (57), (54), (52), (50), (41), (22), (16), (15), (11), (7), (6), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN1176849) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Activated mouse Th1 clone D1.6
Clone MR1
Isotype IgG3, kappa

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN1176849) Images back to top
Antigen
Alternative Name CD154 (CD40LG Antibody Abstract)
Background The MR1 antibody reacts with CD154 (CD40 Ligand, gp39), an accessory molecule expressed on activated T helper (CD4+) lymphocytes. CD154 has also been detected on other types of leukocytes, including CD8+ T cells, medullary thymocytes, activated CD4+ NK-T cells, and human NK cells. CD154 plays an important role in costimulatory interactions between T and B lymphocytes and between antigen-presenting cells and lymphocytes, regulating the immune response at multiple levels. MR1 mAb inhibits in vitro activation of B lymphocytes by T helper cells by blocking interaction of gp39 with CD40. In vitro interactions of T cells and antigen-presenting cells can also be blocked by the MR1 antibody. In vivo treatment with MR1 antibody blocks the development of experimental autoimmune disease, inhibits formation of germinal centers and generation of memory B cells, reduces T-lymphocyte responses to allogeneic cells and allografts, prevents intrathymic deletion of self-reactive T lymphocytes, and disrupts antigen-specific T-cell responses. CD154 expression on activated and resting T lymphocytes. BALB/c spleen T cells, purified on a T Cell Enrichment Column (R&D Systems), were cultured for 8 hours in the presence (Panels A and C) or absence (Panels B and D) of plate-bound 500A2 antibody (anti-CD3e, Cat. No. 553238). They were stained with NA/LE™ MR1 antibody (Panels A and B), followed by biotinylated mouse anti-hamster IgG, Cat. No. 554010, then Streptavidin-PE, Cat. No. 554061, (Panels A, B, C, and D). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Synonyms: CD40 Ligand, gp39
Research Area Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN1176849) Images back to top
Application Notes Flow cytometry: For the detection of mouse CD154 on activated peripheral T cells, it is strongly recommended that T cells be purified before activation. Mouse CD154 is transiently expressed on the surfaces of activated normal T cells and certain T cell clones with a maximal level detected 6-8 hours post-activation. Activation with immobilized anti-CD3e mAb (e.g., 145-2C11, Cat. No. 557306/553058, or 500A2, Cat. No. 553238) is sufficient to induce CD154 expression on CD4+ cells. It has been reported that CD8+ cells express CD154 only in response to PMA/Ionomycin treatment. Therefore, for detection of CD154, it is crucial to utilize the proper activation stimuli and to stain cells at the optimal time for CD154 expression. We recommend the use of biotinylated mouse anti-hamster IgG cocktail (Cat. No. 554010) followed by a bright second-step reagent, such as Streptavidin-PE (Cat. No. 554061), for optimal detection of CD154.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN1176849) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD40 Ligand Antibody (ABIN1176849)

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Product cited in:

Miga, Masters, Durell, Gonzalez, Jenkins, Maliszewski, Kikutani, Wade, Noelle: "Dendritic cell longevity and T cell persistence is controlled by CD154-CD40 interactions." in: European journal of immunology, Vol. 31, Issue 3, pp. 959-65, 2001

Graca, Honey, Adams, Cobbold, Waldmann: "Cutting edge: anti-CD154 therapeutic antibodies induce infectious transplantation tolerance." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 4783-6, 2000

Lettesjö, Burd, Mageed: "CD4+ T lymphocytes with constitutive CD40 ligand in preautoimmune (NZB x NZW)F1 lupus-prone mice: phenotype and possible role in autoreactivity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 4095-104, 2000

Tomura, Yu, Ahn, Yamashita, Yang, Ono, Hamaoka, Kawano, Taniguchi, Koezuka, Fujiwara: "A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 93-101, 1999

Garside, Ingulli, Merica, Johnson, Noelle, Jenkins: "Visualization of specific B and T lymphocyte interactions in the lymph node." in: Science (New York, N.Y.), Vol. 281, Issue 5373, pp. 96-9, 1998

Grewal, Flavell: "CD40 and CD154 in cell-mediated immunity." in: Annual review of immunology, Vol. 16, pp. 111-35, 1998

Kalled, Cutler, Datta, Thomas: "Anti-CD40 ligand antibody treatment of SNF1 mice with established nephritis: preservation of kidney function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2158-65, 1998

Carbone, Ruggiero, Terrazzano, Palomba, Manzo, Fontana, Spits, Kärre, Zappacosta: "A new mechanism of NK cell cytotoxicity activation: the CD40-CD40 ligand interaction." in: The Journal of experimental medicine, Vol. 185, Issue 12, pp. 2053-60, 1997

DeKruyff, Gieni, Umetsu: "Antigen-driven but not lipopolysaccharide-driven IL-12 production in macrophages requires triggering of CD40." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 1, pp. 359-66, 1997

Kelsall, Stüber, Neurath, Strober: "Interleukin-12 production by dendritic cells. The role of CD40-CD40L interactions in Th1 T-cell responses." in: Annals of the New York Academy of Sciences, Vol. 795, pp. 116-26, 1997

Dunn, Luedecker, Haugen, Clegg, Farr: "Thymic overexpression of CD40 ligand disrupts normal thymic epithelial organization." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 45, Issue 1, pp. 129-41, 1997

Kawano, Cui, Koezuka, Toura, Kaneko, Motoki, Ueno, Nakagawa, Sato, Kondo, Koseki, Taniguchi: "CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1626-9, 1997

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, 1997

Griggs, Agersborg, Noelle, Ledbetter, Linsley, Tung: "The relative contribution of the CD28 and gp39 costimulatory pathways in the clonal expansion and pathogenic acquisition of self-reactive T cells." in: The Journal of experimental medicine, Vol. 183, Issue 3, pp. 801-10, 1996

Laman, Claassen, Noelle: "Functions of CD40 and its ligand, gp39 (CD40L)." in: Critical reviews in immunology, Vol. 16, Issue 1, pp. 59-108, 1996

Larsen, Elwood, Alexander, Ritchie, Hendrix, Tucker-Burden, Cho, Aruffo, Hollenbaugh, Linsley, Winn, Pearson: "Long-term acceptance of skin and cardiac allografts after blocking CD40 and CD28 pathways." in: Nature, Vol. 381, Issue 6581, pp. 434-8, 1996

Tian, Noelle, Lawrence: "Activated T cells enhance nitric oxide production by murine splenic macrophages through gp39 and LFA-1." in: European journal of immunology, Vol. 25, Issue 1, pp. 306-9, 1995

Foy, Page, Waldschmidt, Schoneveld, Laman, Masters, Tygrett, Ledbetter, Aruffo, Claassen, Xu, Flavell, Oehen, Hedrick, Noelle: "An essential role for gp39, the ligand for CD40, in thymic selection." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1377-88, 1995

Roy, Aruffo, Ledbetter, Linsley, Kehry, Noelle: "Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses." in: European journal of immunology, Vol. 25, Issue 2, pp. 596-603, 1995

Foy, Laman, Ledbetter, Aruffo, Claassen, Noelle: "gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory." in: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 157-63, 1994

Images

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN1176849) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 Ligand antibody (CD40LG) (ABIN1176849) anti-CD40 Ligand (CD40LG) antibody