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anti-Mouse (Murine) CD40 Ligand antibody for Flow Cytometry

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD154
  • cd40l
  • CD40L
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TNFSF5
  • TRAP
  • gp39
  • hCD40L
  • CD40-L
  • Cd40l
  • Ly-62
  • Ly62
  • Tnfsf5
  • CD40 ligand
  • TNF superfamily member 5
  • CD40LG
  • cd40l
  • Cd40lg
  • LOC100358388
  • CD154
Epitope
AA 61-260
20
16
15
15
14
12
9
8
7
6
5
5
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
458
233
55
20
20
10
8
8
5
4
3
3
3
3
2
1
1
Host
Rat
277
164
113
51
31
28
Clonality
Monoclonal
Conjugate
This CD40 Ligand antibody is un-conjugated
79
61
44
12
12
11
10
10
9
8
8
8
8
8
8
6
6
6
6
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Neutralization (Neut)
395
279
176
95
67
49
46
41
30
20
14
13
10
9
7
6
6
6
5
4
3
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4900437
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.470028 ABIN4897698 FACS PE Rat IgG2a AA 61-260 Log in to see 208109 1
13.470028 ABIN1176849 BR FACS IHC Armenian Hamster IgG3, kappa Log in to see MR1 23
13.470028 ABIN370946 FACS FITC Hamster IgG Log in to see MR1 7
13.470028 ABIN674439 FACS IF (p) IHC (p) Rabbit IgG AA 47-80 Log in to see Polyclonal 1
13.470028 ABIN2659110 FACS PE-Cy7 Hamster IgG Log in to see MR1 8
13.470028 ABIN2663050 FACS PE Hamster IgG Log in to see MR1 10
13.470028 ABIN2659972 FACS PerCP-Cy5.5 Hamster IgG Log in to see MR1 8
13.470028 ABIN2660955 FACS IF IHC Biotin Hamster IgG Log in to see MR1 8
13.470028 ABIN2656942 FACS WB APC Hamster IgG Log in to see MR1 9
13.470028 ABIN2665556 BR FACS IHC Hamster IgG Log in to see MR1 8
13.470028 ABIN4292990 FACS Alexa Fluor 594 Rat IgG2a AA 61-260 Log in to see 208109
13.470028 ABIN4897697 FACS Fluorescein Rat IgG2a AA 61-260 Log in to see 208109
13.470028 ABIN4897696 FACS Allophycocyanin Rat IgG2a AA 61-260 Log in to see 208109
13.470028 ABIN439957 FACS IHC (fro) IP in vitro FITC Armenian Hamster IgG1 kappa Log in to see MR1
13.470028 ABIN2168634 FACS PE Hamster IgG Log in to see Polyclonal
13.470028 ABIN2168631 FACS Biotin Hamster IgG Log in to see Polyclonal
13.470028 ABIN2168632 FACS FITC Hamster IgG Log in to see Polyclonal
13.470028 ABIN2168635 FACS Hamster IgG Log in to see Polyclonal
13.470028 ABIN2168633 FACS Hamster IgG Log in to see Polyclonal
1 ABIN118278 FACS FITC Hamster IgG Log in to see 39H5 2

Top referenced anti-CD40 Ligand antibody for Flow Cytometry

Similar anti-CD40 Ligand Antibodies

Application / Reactivity Mouse (Murine)
Blocking Peptide (BP) 4 Antibodies
Blocking Reagent (BR) 5 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies
ELISA 9 Antibodies
Flow Cytometry (FACS) 185 Antibodies
Functional Studies (Func) 17 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 53 Antibodies
In vitro Assay (in vitro) 1 Antibodies
In vivo Studies (in vivo) 2 Antibodies
Neutralization (Neut) 9 Antibodies
Western Blotting (WB) 38 Antibodies

General

Antigen CD40 Ligand (CD40LG) Antibodies
Epitope AA 61-260
(20), (16), (15), (15), (14), (12), (9), (8), (7), (6), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(458), (233), (55), (20), (20), (10), (8), (8), (5), (4), (3), (3), (3), (3), (2), (1), (1)
Host Rat
(277), (164), (113), (51), (31), (28)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 Ligand antibody is un-conjugated
(79), (61), (44), (12), (12), (11), (10), (10), (9), (8), (8), (8), (8), (8), (8), (6), (6), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Neutralization (Neut)
(395), (279), (176), (95), (67), (49), (46), (41), (30), (20), (14), (13), (10), (9), (7), (6), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN4900437) Images
Specificity Detects mouse CD40 Ligand in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) CD27 Ligand, rmCD30 Ligand, or recombinant human CD40 Ligand is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse CD40 Ligand/TNFSF5, Glu61-Leu260, Accession # P27548
Clone 208109
Isotype IgG2a

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN4900437) Images back to top
Antigen
Alternative Name CD40 Ligand/TNFSF5 (CD40LG Antibody Abstract)
Gene ID 959
UniProt P27548
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN4900437) Images back to top
Application Notes Flow Cytometry 0.25 μg/10^6 cells, Neutralization 0.0075-0.045 μg/mL, CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN4900437) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD40 Ligand Antibody (ABIN4900437)

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Product cited in:

Ito, Ando, Ishikawa, Nakayama, Taniguchi, Saito, Imawari, Moriwaki, Yokochi, Kakumu, Seishima: "Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance." in: International immunology, Vol. 20, Issue 7, pp. 869-79, 2008 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN4900437) back to top
Supplier Images
Neutralization (Neut) image for anti-CD40 Ligand antibody (CD40LG) (AA 61-260) (ABIN4900437) Proliferation Induced by CD40 Ligand/TNFSF5 and Neutralization by Mouse CD40 Ligand/T...
Flow Cytometry (FACS) image for anti-CD40 Ligand antibody (CD40LG) (AA 61-260) (ABIN4900437) Detection of CD40 Ligand/TNFSF5 in Mouse Splenocytes by Flow Cytometry. Mouse splenoc...