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anti-Mouse (Murine) CD40 Ligand antibody for Immunocytochemistry

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD40-L
  • Cd40l
  • CD40L
  • cd40l
  • CD154
  • gp39
  • hCD40L
  • HIGM1
  • IGM
  • IMD3
  • Ly-62
  • Ly62
  • T-BAM
  • Tnfsf5
  • TNFSF5
  • TRAP
Reactivity
Mouse (Murine)
456
211
40
15
14
11
7
4
3
3
3
2
2
2
2
Host
Hamster
286
146
102
57
28
26
Clonality
Monoclonal
Conjugate
This CD40 Ligand antibody is un-conjugated
80
61
43
11
11
9
9
8
8
8
7
7
7
7
7
7
7
6
6
6
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
379
250
171
58
58
54
52
50
41
22
16
16
11
7
6
6
5
4
3
3
3
2
2
1
1
1
1
1
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Catalog No. ABIN1302520
$ 219.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN1302448 Func ICC FACS IP Hamster IgG1 Log in to see MR-1 7
0.0009962841 ABIN1302930 Func ICC FACS IP IHC FITC Hamster IgG1 Log in to see MR-1 8
0.0009962841 ABIN2854749 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.0009962841 ABIN490311 Func ICC FACS IP IHC Hamster IgG1 Log in to see MR1-MR-1
0.0009962841 ABIN490310 Func ICC FACS IP IHC Hamster IgG1 Log in to see MR1-MR-1
0.0009962841 ABIN2962195 ICC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0009962841 ABIN3199492 FACS Func ICC IHC IP FITC Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN956670 ICC Neut ELISA WB Goat IgG Log in to see Polyclonal
0.0009962841 ABIN3199490 FACS Func ICC IHC IP Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN4261055 FACS ICC IF IHC (p) FITC Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN4261057 FACS ICC IF IHC (p) PE Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN4292975 FACS Func ICC IF IHC IHC (p) IP Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN4293012 FACS ICC IF IHC (p) DyLight 755 Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN4293013 FACS ICC IF IHC (p) Alexa Fluor 405 Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN4293014 FACS ICC IF IHC (p) Alexa Fluor 488 Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN4293015 FACS ICC IF IHC (p) Alexa Fluor 647 Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN4293016 FACS ICC IF IHC (p) Alexa Fluor 700 Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN4293017 FACS ICC IF IHC (p) Allophycocyanin Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN4293018 FACS ICC IF IHC (p) Biotin Armenian Hamster IgG1 Log in to see MR-1
0.0009962841 ABIN4293019 FACS ICC IF IHC (p) DyLight 650 Armenian Hamster IgG1 Log in to see MR-1

Top referenced anti-CD40 Ligand antibody for Immunocytochemistry

Similar anti-CD40 Ligand Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 3 Antibodies
ELISA 8 Antibodies
Flow Cytometry (FACS) 168 Antibodies
Functional Studies (Func) 21 Antibodies
Immunocytochemistry (ICC) 34 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 23 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 48 Antibodies
In vitro Assay (in vitro) 3 Antibodies
In vivo Studies (in vivo) 3 Antibodies
Neutralization (Neut) 16 Antibodies
Western Blotting (WB) 32 Antibodies

General

Antigen CD40 Ligand (CD40LG) Antibodies
Reactivity Mouse (Murine)
(456), (211), (40), (15), (14), (11), (7), (4), (3), (3), (3), (2), (2), (2), (2)
Host Hamster
(286), (146), (102), (57), (28), (26)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 Ligand antibody is un-conjugated
(80), (61), (43), (11), (11), (9), (9), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
(379), (250), (171), (58), (58), (54), (52), (50), (41), (22), (16), (16), (11), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN1302520) Images
Specificity The Armenian hamster monoclonal antibody MR-1 detects mouse CD154 / CD40L (CD40-ligand), a 39 kDa cell surface glycoprotein expressed predominantly on activated CD4+ lymphocytes.
Purification Purified by protein G
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen Activated mouse Th1 clone D1.6
Clone MR-1
Isotype IgG1

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN1302520) Images back to top
Antigen
Alternative Name CD40LG (CD40LG Antibody Abstract)
Background CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN1302520) Images back to top
Application Notes Flow Cytometry: Recommended dilution: 4 µg/mL
Positive control: -8 hour activated mouse splenocytes
Immunoprecipitation:
Immunohistochemistry:
Functional Application: Blocking T cell costimulation in vivo.

Working concentrations should be determined by the investigator.
Comment

CD and Related Antigens & MHC (Rodent)

Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN1302520) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD40 Ligand Antibody (ABIN1302520)

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Product cited in:

Shin, Kennedy, Gorski, Tsuchiya, Koseki, Azuma, Yagita, Chen, Powell, Pardoll, Housseau: "Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor." in: The Journal of experimental medicine, Vol. 198, Issue 1, pp. 31-8, 2003

Luzina, Atamas, Storrer, daSilva, Kelsoe, Papadimitriou, Handwerger: "Spontaneous formation of germinal centers in autoimmune mice." in: Journal of leukocyte biology, Vol. 70, Issue 4, pp. 578-84, 2001

Fischbein, Ardehali, Yun, Schoenberger, Laks, Irie, Dempsey, Cheng, Fishbein, Bonavida: "CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 7316-22, 2000

Hwang, Nahm, Briles, Thomas, Purkerson: "Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L." in: Infection and immunity, Vol. 68, Issue 2, pp. 511-7, 2000

Lode, Xiang, Pertl, Förster, Schoenberger, Gillies, Reisfeld: "Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction." in: The Journal of clinical investigation, Vol. 105, Issue 11, pp. 1623-30, 2000

Howard, Miga, Vanderlugt, Dal Canto, Laman, Noelle, Miller: "Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis." in: The Journal of clinical investigation, Vol. 103, Issue 2, pp. 281-90, 1999

Salgado, Nakamura, Sugaya, Tada, Asahina, Koyama, Irie, Tamaki: "Functional CD40 ligand is expressed on epidermal Langerhans cells." in: Journal of leukocyte biology, Vol. 66, Issue 2, pp. 281-5, 1999

Noelle, Roy, Shepherd, Stamenkovic, Ledbetter, Aruffo: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, 1992