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|Application / Reactivity||Mouse (Murine)|
|Blocking Reagent (BR)||3 Antibodies|
|Flow Cytometry (FACS)||110 Antibodies|
|Functional Studies (Func)||20 Antibodies|
|Immunocytochemistry (ICC)||15 Antibodies|
|Immunofluorescence (IF)||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||23 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||47 Antibodies|
|In vitro Assay (in vitro)||3 Antibodies|
|In vivo Studies (in vivo)||3 Antibodies|
|Neutralization (Neut)||2 Antibodies|
|Western Blotting (WB)||32 Antibodies|
|Antigen||CD40 Ligand (CD40LG) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD40 Ligand antibody is un-conjugated Alternatives|
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
|8 references available|
|Supplier||Log in to see|
Product Details anti-CD40 Ligand AntibodyTarget Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN1302520) Images
|Specificity||The Armenian hamster monoclonal antibody MR-1 detects mouse CD154 / CD40L (CD40-ligand), a 39 kDa cell surface glycoprotein expressed predominantly on activated CD4+ lymphocytes.|
|Purification||Purified by protein G|
|Purity||> 95 % (by SDS-PAGE)|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.|
|Immunogen||Activated mouse Th1 clone D1.6|
Target Details CD40 LigandProduct Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN1302520) Images back to top
|Alternative Name||CD40LG (CD40LG Antibody Abstract)|
|Background||CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.|
Application DetailsProduct Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN1302520) Images back to top
Flow Cytometry: Recommended dilution: 4 µg/mL
Positive control: -8 hour activated mouse splenocytes
Functional Application: Blocking T cell costimulation in vivo.
Working concentrations should be determined by the investigator.
CD and Related Antigens & MHC (Rodent)
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN1302520) Images back to top
|Buffer||Azide free phosphate buffered saline (PBS), approx. pH 7.4.|
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 2-8 °C. Do not use after expiration date stamped on vial label.|
References for anti-CD40 Ligand Antibody (ABIN1302520)Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
|Product cited in:||
Shin, Kennedy, Gorski et al.: "Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor." in: The Journal of experimental medicine, Vol. 198, Issue 1, pp. 31-8, 2003 (PubMed).
Luzina, Atamas, Storrer et al.: "Spontaneous formation of germinal centers in autoimmune mice." in: Journal of leukocyte biology, Vol. 70, Issue 4, pp. 578-84, 2001 (PubMed).
Fischbein, Ardehali, Yun et al.: "CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 7316-22, 2000 (PubMed).
Lode, Xiang, Pertl et al.: "Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction." in: The Journal of clinical investigation, Vol. 105, Issue 11, pp. 1623-30, 2000 (PubMed).
Hwang, Nahm, Briles et al.: "Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L." in: Infection and immunity, Vol. 68, Issue 2, pp. 511-7, 2000 (PubMed).
Salgado, Nakamura, Sugaya et al.: "Functional CD40 ligand is expressed on epidermal Langerhans cells." in: Journal of leukocyte biology, Vol. 66, Issue 2, pp. 281-5, 1999 (PubMed).
Howard, Miga, Vanderlugt et al.: "Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis." in: The Journal of clinical investigation, Vol. 103, Issue 2, pp. 281-90, 1999 (PubMed).
Noelle, Roy, Shepherd et al.: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, 1992 (PubMed).