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anti-Mouse (Murine) CD40 Ligand antibody for Immunofluorescence

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD40-L
  • Cd40l
  • CD40L
  • cd40l
  • CD154
  • gp39
  • hCD40L
  • HIGM1
  • IGM
  • IMD3
  • Ly-62
  • Ly62
  • T-BAM
  • Tnfsf5
  • TNFSF5
  • TRAP
Reactivity
Mouse (Murine)
366
151
40
15
14
11
7
4
3
3
3
2
2
2
2
Host
Hamster
219
123
88
26
21
19
Clonality
Monoclonal
Conjugate
This CD40 Ligand antibody is conjugated to Biotin
72
53
36
8
6
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
270
190
135
52
51
49
40
31
21
15
11
7
6
6
6
5
4
3
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN2660955
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010843921 ABIN2854749 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.0010843921 ABIN3036741 Func ICC FACS IF IP IHC Hamster IgG1 Log in to see
0.0010843921 ABIN3036742 Func ICC FACS IF IP IHC FITC Hamster IgG1 Log in to see
0.0010843921 ABIN3036740 Func ICC FACS IF IP IHC Hamster IgG1 Log in to see

Top referenced anti-CD40 Ligand antibody for Immunofluorescence

Similar anti-CD40 Ligand Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 3 Antibodies
ELISA 8 Antibodies
Flow Cytometry (FACS) 109 Antibodies
Functional Studies (Func) 20 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 22 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 47 Antibodies
In vitro Assay (in vitro) 3 Antibodies
In vivo Studies (in vivo) 3 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 32 Antibodies

General

Antigen CD40 Ligand (CD40LG) Antibodies
Reactivity Mouse (Murine)
(366), (151), (40), (15), (14), (11), (7), (4), (3), (3), (3), (2), (2), (2), (2)
Host Hamster
(219), (123), (88), (26), (21), (19)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 Ligand antibody is conjugated to Biotin
(72), (53), (36), (8), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
(270), (190), (135), (52), (51), (49), (40), (31), (21), (15), (11), (7), (6), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN2660955) Images
Purification The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Clone MR1
Isotype IgG

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN2660955) Images back to top
Antigen
Alternative Name CD154 (CD40LG Antibody Abstract)
Background CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. The MR1 antibody has been reported to inhibit the activation of T and B lymphocytes in vitro and antigen-specific lymphocyte responses in vivo.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN2660955) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN2660955) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD40 Ligand Antibody (ABIN2660955)

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Background publications

Lawson, Manenkova, Ahamed, Chen, Zou, Baccala, Theofilopoulos, Yuan: "Inhibition of transmethylation down-regulates CD4 T cell activation and curtails development of autoimmunity in a model system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 5366-74, 2007

Lettesjö, Burd, Mageed: "CD4+ T lymphocytes with constitutive CD40 ligand in preautoimmune (NZB x NZW)F1 lupus-prone mice: phenotype and possible role in autoreactivity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 4095-104, 2000

Dunn, Luedecker, Haugen, Clegg, Farr: "Thymic overexpression of CD40 ligand disrupts normal thymic epithelial organization." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 45, Issue 1, pp. 129-41, 1997

Roy, Aruffo, Ledbetter, Linsley, Kehry, Noelle: "Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses." in: European journal of immunology, Vol. 25, Issue 2, pp. 596-603, 1995

Banchereau, Bazan, Blanchard, Brière, Galizzi, van Kooten, Liu, Rousset, Saeland: "The CD40 antigen and its ligand." in: Annual review of immunology, Vol. 12, pp. 881-922, 1994

Foy, Laman, Ledbetter, Aruffo, Claassen, Noelle: "gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory." in: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 157-63, 1994

Noelle, Roy, Shepherd, Stamenkovic, Ledbetter, Aruffo: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, 1992

Clark, Ledbetter: "Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 12, pp. 4494-8, 1986

Images

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN2660955) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 Ligand antibody (CD40LG)  (Biotin) (ABIN2660955) anti-CD40 Ligand (CD40LG) antibody (Biotin)