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|Application / Reactivity||Mouse (Murine)|
|Blocking Reagent (BR)||3 Antibodies|
|Flow Cytometry (FACS)||110 Antibodies|
|Functional Studies (Func)||20 Antibodies|
|Immunocytochemistry (ICC)||15 Antibodies|
|Immunofluorescence (IF)||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||23 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||47 Antibodies|
|In vitro Assay (in vitro)||3 Antibodies|
|In vivo Studies (in vivo)||3 Antibodies|
|Neutralization (Neut)||2 Antibodies|
|Western Blotting (WB)||32 Antibodies|
|Antigen||CD40 Ligand (CD40LG) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD40 Ligand antibody is conjugated to Biotin Alternatives|
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
|8 references available|
|Supplier||Log in to see|
Product Details anti-CD40 Ligand AntibodyTarget Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN2660955) Images
|Purification||The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.|
Target Details CD40 LigandProduct Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN2660955) Images back to top
|Alternative Name||CD154 (CD40LG Antibody Abstract)|
|Background||CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. The MR1 antibody has been reported to inhibit the activation of T and B lymphocytes in vitro and antigen-specific lymphocyte responses in vivo.|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN2660955) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN2660955) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-CD40 Ligand Antibody (ABIN2660955)Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Lawson, Manenkova, Ahamed et al.: "Inhibition of transmethylation down-regulates CD4 T cell activation and curtails development of autoimmunity in a model system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 5366-74, 2007 (PubMed).
Lettesjö, Burd, Mageed: "CD4+ T lymphocytes with constitutive CD40 ligand in preautoimmune (NZB x NZW)F1 lupus-prone mice: phenotype and possible role in autoreactivity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 4095-104, 2000 (PubMed).
Dunn, Luedecker, Haugen et al.: "Thymic overexpression of CD40 ligand disrupts normal thymic epithelial organization." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 45, Issue 1, pp. 129-41, 1997 (PubMed).
Roy, Aruffo, Ledbetter et al.: "Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses." in: European journal of immunology, Vol. 25, Issue 2, pp. 596-603, 1995 (PubMed).
Banchereau, Bazan, Blanchard et al.: "The CD40 antigen and its ligand." in: Annual review of immunology, Vol. 12, pp. 881-922, 1994 (PubMed).
Foy, Laman, Ledbetter et al.: "gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory." in: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 157-63, 1994 (PubMed).
Clark, Ledbetter: "Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 12, pp. 4494-8, 1986 (PubMed).
Noelle, Roy, Shepherd et al.: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, 1992 (PubMed).
ImagesProduct Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN2660955) back to top