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anti-Mouse (Murine) CD40 Ligand antibody for Immunohistochemistry (Frozen Sections)

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD40-L
  • Cd40l
  • CD40L
  • cd40l
  • CD154
  • gp39
  • hCD40L
  • HIGM1
  • IGM
  • IMD3
  • Ly-62
  • Ly62
  • T-BAM
  • Tnfsf5
  • TNFSF5
  • TRAP
Reactivity
Mouse (Murine)
456
211
40
15
14
11
7
4
3
3
3
2
2
2
2
Host
Armenian Hamster
286
146
103
56
28
26
Clonality
Monoclonal
Conjugate
This CD40 Ligand antibody is un-conjugated
80
61
43
11
11
9
9
8
8
8
7
7
7
7
7
7
7
6
6
6
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
379
250
171
59
58
54
53
51
41
22
16
15
11
7
6
5
5
4
3
3
3
2
2
1
1
1
1
1
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Catalog No. ABIN2688993
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000883884 ABIN439957 FACS IHC (fro) IP in vitro FITC Armenian Hamster IgG1 kappa Log in to see MR1

Top referenced anti-CD40 Ligand antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CD40 Ligand Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 3 Antibodies
ELISA 8 Antibodies
Flow Cytometry (FACS) 168 Antibodies
Functional Studies (Func) 21 Antibodies
Immunocytochemistry (ICC) 34 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 23 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 48 Antibodies
In vitro Assay (in vitro) 3 Antibodies
In vivo Studies (in vivo) 3 Antibodies
Neutralization (Neut) 16 Antibodies
Western Blotting (WB) 32 Antibodies

General

Antigen CD40 Ligand (CD40LG) Antibodies
Reactivity Mouse (Murine)
(456), (211), (40), (15), (14), (11), (7), (4), (3), (3), (3), (2), (2), (2), (2)
Host Armenian Hamster
(286), (146), (103), (56), (28), (26)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 Ligand antibody is un-conjugated
(80), (61), (43), (11), (11), (9), (9), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(379), (250), (171), (59), (58), (54), (53), (51), (41), (22), (16), (15), (11), (7), (6), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN2688993) Images
Brand BD Pharmingen™
Characteristics The MR1 antibody reacts with CD154 (CD40 Ligand, gp39), an accessory molecule expressed on activated T helper (CD4+) lymphocytes. CD154 has also been detected on other types of leukocytes, including CD8+ T cells, medullary thymocytes, activated CD4+ NK-T cells, and human NK cells. CD154 plays an important role in costimulatory interactions between T and B lymphocytes and between antigen-presenting cells and lymphocytes, regulating the immune response at multiple levels. MR1 mAb inhibits in vitro activation of B lymphocytes by T helper cells by blocking interaction of gp39 with CD40. In vitro interactions of T cells and antigen-presenting cells can also be blocked by the MR1 antibody. In vivo treatment with MR1 antibody blocks the development of experimental autoimmune disease, inhibits formation of germinal centers and generation of memory B cells, reduces T-lymphocyte responses to allogeneic cells and allografts, prevents intrathymic deletion of self-reactive T lymphocytes, and disrupts antigen-specific T-cell responses. CD154 expression on activated and resting T lymphocytes. BALB/c spleen T cells, purified on a T Cell Enrichment Column (R&D Systems), were cultured for 8 hours in the presence (Panels A and C) or absence (Panel B) of plate-bound 500A2 antibody (anti-CD3e, Cat. No. 553238). They were stained with purified MR1 antibody (Panels A and B), followed by biotinylated mouse anti-hamster IgG, Cat. No. 554010, then Streptavidin-PE, Cat. No. 554061, (Panels A, B, and C). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Purified Hamster Anti-Mouse CD154 - Purified - Clone MR1 - Isotype Armenian Hamster IgG3, κ - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Activated mouse Th1 clone D1.6
Clone MR1
Isotype IgG3, kappa

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN2688993) Images back to top
Antigen
Alternative Name CD154 (CD40LG Antibody Abstract)
Background Synonyms: CD40 Ligand, gp39
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN2688993) Images back to top
Application Notes Flow Cytometry System.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN2688993) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD40 Ligand Antibody (ABIN2688993)

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Product cited in:

Miga, Masters, Durell, Gonzalez, Jenkins, Maliszewski, Kikutani, Wade, Noelle: "Dendritic cell longevity and T cell persistence is controlled by CD154-CD40 interactions." in: European journal of immunology, Vol. 31, Issue 3, pp. 959-65, 2001

Graca, Honey, Adams, Cobbold, Waldmann: "Cutting edge: anti-CD154 therapeutic antibodies induce infectious transplantation tolerance." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 4783-6, 2000

Lettesjö, Burd, Mageed: "CD4+ T lymphocytes with constitutive CD40 ligand in preautoimmune (NZB x NZW)F1 lupus-prone mice: phenotype and possible role in autoreactivity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 4095-104, 2000

Tomura, Yu, Ahn, Yamashita, Yang, Ono, Hamaoka, Kawano, Taniguchi, Koezuka, Fujiwara: "A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 93-101, 1999

Garside, Ingulli, Merica, Johnson, Noelle, Jenkins: "Visualization of specific B and T lymphocyte interactions in the lymph node." in: Science (New York, N.Y.), Vol. 281, Issue 5373, pp. 96-9, 1998

Grewal, Flavell: "CD40 and CD154 in cell-mediated immunity." in: Annual review of immunology, Vol. 16, pp. 111-35, 1998

Kalled, Cutler, Datta, Thomas: "Anti-CD40 ligand antibody treatment of SNF1 mice with established nephritis: preservation of kidney function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2158-65, 1998

Carbone, Ruggiero, Terrazzano, Palomba, Manzo, Fontana, Spits, Kärre, Zappacosta: "A new mechanism of NK cell cytotoxicity activation: the CD40-CD40 ligand interaction." in: The Journal of experimental medicine, Vol. 185, Issue 12, pp. 2053-60, 1997

DeKruyff, Gieni, Umetsu: "Antigen-driven but not lipopolysaccharide-driven IL-12 production in macrophages requires triggering of CD40." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 1, pp. 359-66, 1997

Dunn, Luedecker, Haugen, Clegg, Farr: "Thymic overexpression of CD40 ligand disrupts normal thymic epithelial organization." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 45, Issue 1, pp. 129-41, 1997

Kawano, Cui, Koezuka, Toura, Kaneko, Motoki, Ueno, Nakagawa, Sato, Kondo, Koseki, Taniguchi: "CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1626-9, 1997

Kelsall, Stüber, Neurath, Strober: "Interleukin-12 production by dendritic cells. The role of CD40-CD40L interactions in Th1 T-cell responses." in: Annals of the New York Academy of Sciences, Vol. 795, pp. 116-26, 1997

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, 1997

Griggs, Agersborg, Noelle, Ledbetter, Linsley, Tung: "The relative contribution of the CD28 and gp39 costimulatory pathways in the clonal expansion and pathogenic acquisition of self-reactive T cells." in: The Journal of experimental medicine, Vol. 183, Issue 3, pp. 801-10, 1996

Laman, Claassen, Noelle: "Functions of CD40 and its ligand, gp39 (CD40L)." in: Critical reviews in immunology, Vol. 16, Issue 1, pp. 59-108, 1996

Larsen, Elwood, Alexander, Ritchie, Hendrix, Tucker-Burden, Cho, Aruffo, Hollenbaugh, Linsley, Winn, Pearson: "Long-term acceptance of skin and cardiac allografts after blocking CD40 and CD28 pathways." in: Nature, Vol. 381, Issue 6581, pp. 434-8, 1996

Foy, Page, Waldschmidt, Schoneveld, Laman, Masters, Tygrett, Ledbetter, Aruffo, Claassen, Xu, Flavell, Oehen, Hedrick, Noelle: "An essential role for gp39, the ligand for CD40, in thymic selection." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1377-88, 1995

Roy, Aruffo, Ledbetter, Linsley, Kehry, Noelle: "Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses." in: European journal of immunology, Vol. 25, Issue 2, pp. 596-603, 1995

Tian, Noelle, Lawrence: "Activated T cells enhance nitric oxide production by murine splenic macrophages through gp39 and LFA-1." in: European journal of immunology, Vol. 25, Issue 1, pp. 306-9, 1995

Foy, Laman, Ledbetter, Aruffo, Claassen, Noelle: "gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory." in: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 157-63, 1994