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anti-Mouse (Murine) CD40 Ligand antibody for Immunoprecipitation

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD154
  • cd40l
  • CD40L
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TNFSF5
  • TRAP
  • gp39
  • hCD40L
  • CD40-L
  • Cd40l
  • Ly-62
  • Ly62
  • Tnfsf5
  • CD40 ligand
  • TNF superfamily member 5
  • CD40LG
  • cd40l
  • Cd40lg
  • LOC100358388
  • CD154
Reactivity
Mouse (Murine)
449
231
55
20
20
10
8
8
5
4
3
3
3
3
2
1
1
Host
Hamster
273
165
103
51
35
26
Clonality
Monoclonal
Conjugate
This CD40 Ligand antibody is un-conjugated
73
58
42
14
14
12
12
11
9
8
8
8
8
8
8
6
6
6
6
5
5
5
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
392
269
170
93
65
48
46
39
30
20
15
13
10
9
6
6
6
5
4
4
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN1302520
$ 219.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1302448 Func ICC FACS IP Hamster IgG1 Log in to see MR-1 7
1 ABIN135217 FACS IP Biotin Hamster IgG Log in to see MR1 7
1 ABIN370947 Func FACS IP Hamster IgG Log in to see MR1 7
1 ABIN1302930 Func ICC FACS IP IHC FITC Hamster IgG1 Log in to see MR-1 8
1 ABIN135215 FACS IP Hamster IgG Log in to see MR1 7
1 ABIN135219 FACS IP Hamster IgG Log in to see MR1 7
1 ABIN370948 Func FACS IP Hamster IgG Log in to see MR1 7
1 ABIN439957 FACS IHC (fro) IP in vitro FITC Armenian Hamster IgG1 kappa Log in to see MR1
1 ABIN1862301 ICC IHC IP WB Rabbit IgG AA 50-243 Log in to see Polyclonal
1 ABIN198965 Func FACS IP PE Hamster IgG, kappa Log in to see MR1
1 ABIN490310 BR FACS Func ICC IHC IP Neut Hamster IgG1 Log in to see MR1-MR-1
1 ABIN226480 Func FACS IP FITC Hamster IgG, kappa Log in to see MR1
1 ABIN226481 Func FACS IP Biotin Hamster IgG, kappa Log in to see MR1
1 ABIN1495079 IP Magnetic Particles Rabbit N-Term Log in to see Polyclonal
1 ABIN1495080 IP Sepharose Rabbit N-Term Log in to see Polyclonal
1 ABIN2593288 FACS IP Armenian Hamster IgG Log in to see MR1
1 ABIN2618582 FACS IP Biotin Armenian Hamster IgG Log in to see MR1
1 ABIN490311 BR FACS Func ICC IHC IP Neut Hamster IgG1 Log in to see MR1-MR-1
1 ABIN576386 FACS IP PE Hamster IgG Log in to see
1 ABIN4958047 FACS IP Armenian Hamster IgG Log in to see 39H5

Top referenced anti-CD40 Ligand antibody for Immunoprecipitation

Similar anti-CD40 Ligand Antibodies

Application / Reactivity Mouse (Murine)
Blocking Peptide (BP) 4 Antibodies
Blocking Reagent (BR) 5 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies
ELISA 9 Antibodies
Flow Cytometry (FACS) 183 Antibodies
Functional Studies (Func) 16 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 52 Antibodies
In vitro Assay (in vitro) 1 Antibodies
In vivo Studies (in vivo) 2 Antibodies
Neutralization (Neut) 9 Antibodies
Western Blotting (WB) 38 Antibodies

General

Antigen CD40 Ligand (CD40LG) Antibodies
Reactivity Mouse (Murine)
(449), (231), (55), (20), (20), (10), (8), (8), (5), (4), (3), (3), (3), (3), (2), (1), (1)
Host Hamster
(273), (165), (103), (51), (35), (26)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 Ligand antibody is un-conjugated
(73), (58), (42), (14), (14), (12), (12), (11), (9), (8), (8), (8), (8), (8), (8), (6), (6), (6), (6), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
(392), (269), (170), (93), (65), (48), (46), (39), (30), (20), (15), (13), (10), (9), (6), (6), (6), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN1302520) Images
Specificity The Armenian hamster monoclonal antibody MR-1 detects mouse CD154 / CD40L (CD40-ligand), a 39 kDa cell surface glycoprotein expressed predominantly on activated CD4+ lymphocytes.
Purification Purified by protein G
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen Activated mouse Th1 clone D1.6
Clone MR-1
Isotype IgG1

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN1302520) Images back to top
Antigen
Alternative Name CD40LG (CD40LG Antibody Abstract)
Background CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
Pathways NF-kappaB Signaling, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN1302520) Images back to top
Application Notes Flow Cytometry: Recommended dilution: 4 µg/mL
Positive control: -8 hour activated mouse splenocytes
Immunoprecipitation:
Immunohistochemistry:
Functional Application: Blocking T cell costimulation in vivo.

Working concentrations should be determined by the investigator.
Comment

CD and Related Antigens & MHC (Rodent)

Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN1302520) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD40 Ligand Antibody (ABIN1302520)

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Product cited in:

Shin, Kennedy, Gorski, Tsuchiya, Koseki, Azuma, Yagita, Chen, Powell, Pardoll, Housseau: "Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor." in: The Journal of experimental medicine, Vol. 198, Issue 1, pp. 31-8, 2003

Luzina, Atamas, Storrer, daSilva, Kelsoe, Papadimitriou, Handwerger: "Spontaneous formation of germinal centers in autoimmune mice." in: Journal of leukocyte biology, Vol. 70, Issue 4, pp. 578-84, 2001

Fischbein, Ardehali, Yun, Schoenberger, Laks, Irie, Dempsey, Cheng, Fishbein, Bonavida: "CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 7316-22, 2000

Hwang, Nahm, Briles, Thomas, Purkerson: "Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L." in: Infection and immunity, Vol. 68, Issue 2, pp. 511-7, 2000

Lode, Xiang, Pertl, Förster, Schoenberger, Gillies, Reisfeld: "Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction." in: The Journal of clinical investigation, Vol. 105, Issue 11, pp. 1623-30, 2000

Howard, Miga, Vanderlugt, Dal Canto, Laman, Noelle, Miller: "Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis." in: The Journal of clinical investigation, Vol. 103, Issue 2, pp. 281-90, 1999

Salgado, Nakamura, Sugaya, Tada, Asahina, Koyama, Irie, Tamaki: "Functional CD40 ligand is expressed on epidermal Langerhans cells." in: Journal of leukocyte biology, Vol. 66, Issue 2, pp. 281-5, 1999

Noelle, Roy, Shepherd, Stamenkovic, Ledbetter, Aruffo: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, 1992