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Mouse (Murine) CD40 Ligand ELISA Kit

Recommended CD40 Ligand Product (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) ELISA Kits
  • CD40-L
  • Cd40l
  • CD40L
  • cd40l
  • CD154
  • gp39
  • hCD40L
  • HIGM1
  • IGM
  • IMD3
  • Ly-62
  • Ly62
  • T-BAM
  • Tnfsf5
  • TNFSF5
  • TRAP
Epitope
AA 61-260
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
23
13
6
5
4
3
3
2
2
2
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1889305
$ 399.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.31757757 ABIN415533 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 1
0.0012704609 ABIN366338 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 0.469-30 ng/mL 0.469 ng/mL Log in to see 1
0.0012704609 ABIN772890 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012704609 ABIN456425 Sandwich ELISA Serum, Plasma 78-5000 pg/mL 78 pg/mL Log in to see
0.0012704609 ABIN1029801 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012704609 ABIN509771 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012704609 ABIN2748773 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012704609 ABIN2683744 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012704609 ABIN2683743 Sandwich ELISA Cell Culture Supernatant, Serum 0.31-20 ng/mL 0.31 ng/mL Log in to see
0.0012704609 ABIN1033882 Sandwich ELISA Cell Culture Supernatant, Serum Log in to see
0.0012704609 ABIN2869906 Log in to see
0.0012704609 ABIN625378 Sandwich ELISA Log in to see
0.0012704609 ABIN2072118 Sandwich ELISA Log in to see

Top referenced CD40 Ligand ELISA Kit for ELISA

Similar CD40 Ligand ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 14 ELISA Kits

General

Antigen CD40 Ligand (CD40LG) ELISA Kits
Epitope AA 61-260
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(23), (13), (6), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details CD40 Ligand ELISA Kit

Target details Application Details Handling References for CD40 Ligand Kit (ABIN1889305) Images
Purpose For quantitative detection of mouse CD40L in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse CD40L
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse CD40L antibod - 1
  • Lyophilized recombinant mouse CD40L standar - 10ng/tubex2
  • Biotinylated anti- mouse CD40L antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: E61-L260

Target details

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Antigen
Alternative Name CD40LG (CD40LG ELISA Kit Abstract)
Background CD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
Gene ID 21947
UniProt P27548
Pathways NF-kappaB Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse CD40L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD40L has been precoated onto 96-well plates. Standards(NSO, E61-L260) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD40L amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD40L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD40L standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 537, Standard deviation: 28.5, CV(%): 5.3
  • Sample 2: n=16, Mean(pg/ml): 1562, Standard deviation: 71.9, CV(%): 4.6
  • Sample 3: n=16, Mean(pg/ml): 3025, Standard deviation: 172.4, CV(%): 5.7,
  • Sample 1: n=24, Mean(pg/ml): 562, Standard deviation: 36.53, CV(%): 6.5
  • Sample 2: n=24, Mean(pg/ml): 1675, Standard deviation: 97.15, CV(%): 5.8
  • Sample 3: n=24, Mean(pg/ml): 3140, Standard deviation: 207.24, CV(%): 6.6
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for CD40 Ligand Kit (ABIN1889305)

Product Details CD40 Ligand ELISA Kit Target details Application Details Handling Images back to top
Background publications

Cipollone, Ferri, Desideri, Paloscia, Materazzo, Mascellanti, Fazia, Iezzi, Cuccurullo, Pini, Bucci, Santucci, Cuccurullo, Mezzetti: "Preprocedural level of soluble CD40L is predictive of enhanced inflammatory response and restenosis after coronary angioplasty." in: Circulation, Vol. 108, Issue 22, pp. 2776-82, 2003

Allen, Armitage, Conley, Rosenblatt, Jenkins, Copeland, Bedell, Edelhoff, Disteche, Simoneaux: "CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome." in: Science (New York, N.Y.), Vol. 259, Issue 5097, pp. 990-3, 1993

Images

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Supplier Images
ELISA image for CD40 Ligand ELISA Kit (CD40LG) (ABIN1889305) Mouse CD40L PicoKine ELISA Kit standard curve