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anti-Human EGF antibody for Enzyme Immunoassay

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • AI790464
  • CEGF
  • HOMG4
  • URG
Epitope
AA 689-720, Middle Region
19
14
14
13
11
10
8
7
5
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
276
104
59
15
12
7
4
3
2
1
Host
Rabbit
262
123
29
10
7
3
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
47
24
21
8
8
8
4
4
4
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
271
268
70
47
46
27
26
22
20
15
11
8
6
5
4
3
2
2
2
2
1
Supplier
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Catalog No. ABIN952036
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000882256 ABIN1107062 EIA IF WB Mouse IgG1 Log in to see 4-00E-011 3
0.000882256 ABIN115991 EIA IHC (fro) IHC (p) WB Biotin Rabbit Log in to see Polyclonal
0.000882256 ABIN115992 EIA IHC (fro) IHC (p) WB Biotin Rabbit Log in to see Polyclonal
0.000882256 ABIN452468 EIA HRP Mouse IgG1 Log in to see S-146
0.000882256 ABIN115993 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
0.000882256 ABIN115994 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
0.000882256 ABIN452465 EIA Mouse IgG1 Log in to see S-21
0.000882256 ABIN452467 EIA Mouse IgG1 Log in to see S-145
0.000882256 ABIN452470 EIA Mouse IgG1 Log in to see S-147
0.000882256 ABIN452471 EIA Mouse IgG1 Log in to see S-177
0.000882256 ABIN452466 EIA Mouse IgG1 Log in to see S-120
0.000882256 ABIN1107059 EIA Mouse IgG2a Log in to see KT2
0.000882256 ABIN1107061 EIA IHC (p) WB Mouse IgG1 Log in to see 9D7F11
0.000882256 ABIN1504136 ELISA (Detection) EIA Biotin Mouse IgG1 Log in to see S-146
0.000882256 ABIN613069 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal
0.000882256 ABIN2719963 DB EIA WB Rabbit IgG Log in to see Polyclonal
0.000882256 ABIN613539 EIA RIA Rabbit IgG Log in to see Polyclonal

Similar anti-EGF Antibodies

Application / Reactivity Mouse (Murine) Human
Blocking Antibody (Inhibition) 1 Antibodies 3 Antibodies
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 40 Antibodies 192 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 5 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 73 Antibodies
ELISA (Capture) 2 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope AA 689-720, Middle Region
(19), (14), (14), (13), (11), (10), (8), (7), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(276), (104), (59), (15), (12), (7), (4), (3), (2), (1)
Host Rabbit
(262), (123), (29), (10), (7), (3)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(47), (24), (21), (8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(271), (268), (70), (47), (46), (27), (26), (22), (20), (15), (11), (8), (6), (5), (4), (3), (2), (2), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN952036) Images
Specificity This antibody reacts to EGF.
Cross-Reactivity (Details) Species reactivity (tested):Human and Mouse.
Purification Affinity chromatography on Protein A
Immunogen KLH conjugated synthetic peptide between 689-720 amino acids from the Central region of human EGF
Isotype Ig

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF Antibody (ABIN952036) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Molecular Weight 133994 Da
Gene ID 1950
NCBI Accession NP_001954
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF Antibody (ABIN952036) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF Antibody (ABIN952036) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-EGF Antibody (ABIN952036)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Background publications

Medts, de Diesbach, Cominelli, NKuli, Tyteca, Courtoy: "Acute ligand-independent Src activation mimics low EGF-induced EGFR surface signalling and redistribution into recycling endosomes." in: Experimental cell research, Vol. 316, Issue 19, pp. 3239-53, 2010

Xu, Jiang, Steed, Davidge, Fu: "TGFβ and EGF synergistically induce a more invasive phenotype of epithelial ovarian cancer cells." in: Biochemical and biophysical research communications, Vol. 401, Issue 3, pp. 376-81, 2010

Lupien, Meyer, Bailey, Eeckhoute, Cook, Westerling, Zhang, Carroll, Rhodes, Liu, Brown: "Growth factor stimulation induces a distinct ER(alpha) cistrome underlying breast cancer endocrine resistance." in: Genes & development, Vol. 24, Issue 19, pp. 2219-27, 2010

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN952036) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGF antibody (Epidermal Growth Factor) (AA 689-720) (ABIN952036) EGF Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin em...
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (AA 689-720) (ABIN952036) EGF Antibody (Center) western blot analysis in mouse stomach tissue lysates (35µg/lan...