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anti-Human EGF antibody for ELISA

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
  • LOC100008808
Epitope
AA 971-1023
27
15
15
12
11
11
10
9
8
8
8
7
5
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
324
143
87
19
14
7
4
2
2
1
1
1
Host
Rabbit
296
143
29
10
6
4
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
46
24
21
10
8
8
6
6
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
1
1
1
1
Application
ELISA, Western Blotting (WB)
334
279
108
76
54
33
27
24
19
19
17
14
10
7
6
5
4
2
2
2
2
1
1
Options
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Catalog No. ABIN3044516
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN969091 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11 3
1 ABIN2869485 ELISA ICC WB Mouse IgG1 Log in to see 4E11 4
1 ABIN2869465 ELISA IHC WB Mouse IgG1 Log in to see 9D7F11 4
1 ABIN1724732 ICC ELISA WB Mouse IgG1 Log in to see 4-00E-11 2
1 ABIN3184415 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN574050 IHC (p) ELISA WB Mouse IgG1 Log in to see 9D7F11
1 ABIN1997183 ELISA Rabbit IgG AA 971-1023 Log in to see 008 1
1 ABIN966030 IF ELISA WB Mouse IgG1 Log in to see 9D7F11 4
1 ABIN966031 IHC ELISA WB Mouse IgG1 Log in to see 4E11 4
1 ABIN2470896 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11
1 ABIN2473398 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 1
1 ABIN465856 ELISA IHC Neut WB Biotin Rabbit Log in to see Polyclonal
1 ABIN205919 Func IHC (p) ELISA WB Rabbit IgG Extracellular Domain Log in to see Polyclonal
1 ABIN1824611 ELISA HRP Mouse IgG2a Log in to see KT2
1 ABIN465547 IHC Neut ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1830479 ELISA Biotin Mouse IgG1, kappa Log in to see 12K258
1 ABIN4307156 ELISA ICC IF WB Mouse IgG1 Log in to see 4E11
1 ABIN3201442 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 609-751 Log in to see
1 ABIN259891 IHC (p) IHC ELISA Mouse IgG1 Log in to see EGF-10
1 ABIN191199 ELISA Mouse IgG1 Log in to see EGF-2

Top referenced anti-EGF antibody for ELISA

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General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope AA 971-1023
(27), (15), (15), (12), (11), (11), (10), (9), (8), (8), (8), (7), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(324), (143), (87), (19), (14), (7), (4), (2), (2), (1), (1), (1)
Host Rabbit
(296), (143), (29), (10), (6), (4)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(46), (24), (21), (10), (8), (8), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(334), (279), (108), (76), (54), (33), (27), (24), (19), (19), (17), (14), (10), (7), (6), (5), (4), (2), (2), (2), (2), (1), (1)
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(EGF) detection. Tested with WB, ELISA in Human.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(EGF) detection. Tested with WB, ELISA in Human.
Gene Name: epidermal growth factor
Protein Name: Pro-epidermal growth factor
Purification Immunogen affinity purified.
Immunogen E. coli-derived human EGF recombinant protein (Position: N971-R1023). Human EGF shares 69.8% amino acid (aa) sequence identity with both mouse and rat EGF.
Isotype IgG

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Synonyms: Beta urogastrone antibody|EGF antibody|Egf antibody|EGF_HUMAN antibody|Epidermal Growth Factor antibody|Epidermal Growth Factor antibody|HOMG4 antibody|OTTHUMP00000219721 antibody|OTTHUMP00000219722 antibody|Pro epidermal growth factor antibody|URG antibody| Urogastrone antibody
Gene ID 1950
UniProt P01133
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (AA 971-1023) (ABIN3044516) Western blot analysis of EGF expression in 22RV1 whole cell lysates (lane 1). EGF at ...