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anti-Human EGF antibody for ELISA

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
  • LOC100008808
Reactivity
Human
316
140
85
18
13
7
4
2
1
1
1
Host
Mouse
288
142
29
10
6
4
Clonality
Monoclonal
Conjugate
This EGF antibody is un-conjugated
46
24
21
10
8
8
6
6
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
325
278
107
70
54
33
27
24
19
19
17
14
10
7
6
5
4
2
2
2
2
1
1
Options
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Catalog No. ABIN969091
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1724732 ICC ELISA WB Mouse IgG1 Log in to see 4-00E-11 2
1 ABIN2869465 ELISA IHC WB Mouse IgG1 Log in to see 9D7F11 4
1 ABIN2869485 ELISA ICC WB Mouse IgG1 Log in to see 4E11 4
1 ABIN3044516 ELISA WB Rabbit IgG AA 971-1023 Log in to see Polyclonal
1 ABIN3184415 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN574050 IHC (p) ELISA WB Mouse IgG1 Log in to see 9D7F11
1 ABIN1997183 ELISA Rabbit IgG AA 971-1023 Log in to see 008 1
1 ABIN966030 IF ELISA WB Mouse IgG1 Log in to see 9D7F11 4
1 ABIN966031 IHC ELISA WB Mouse IgG1 Log in to see 4E11 4
1 ABIN2470896 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11
1 ABIN2473398 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 1
1 ABIN465856 ELISA IHC Neut WB Biotin Rabbit Log in to see Polyclonal
1 ABIN3201442 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 609-751 Log in to see
1 ABIN205919 Func IHC (p) ELISA WB Rabbit IgG Extracellular Domain Log in to see Polyclonal
1 ABIN4307156 ELISA ICC IF WB Mouse IgG1 Log in to see 4E11
1 ABIN1830479 ELISA Biotin Mouse IgG1, kappa Log in to see 12K258
1 ABIN1824611 ELISA HRP Mouse IgG2a Log in to see KT2
1 ABIN465547 IHC Neut ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1453236 IHC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN191199 ELISA Mouse IgG1 Log in to see EGF-2

Top referenced anti-EGF antibody for ELISA

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Application / Reactivity Human
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Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human
(316), (140), (85), (18), (13), (7), (4), (2), (1), (1), (1)
Host Mouse
(288), (142), (29), (10), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGF antibody is un-conjugated
(46), (24), (21), (10), (8), (8), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(325), (278), (107), (70), (54), (33), (27), (24), (19), (19), (17), (14), (10), (7), (6), (5), (4), (2), (2), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN969091) Images
Purification Ascites
Immunogen Purified recombinant fragment of human EGF expressed in E. coli.
Clone 9D7F11
Isotype IgG1

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF Antibody (ABIN969091) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Molecular Weight 134 kDa
Gene ID 1950
HGNC 1950
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF Antibody (ABIN969091) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF Antibody (ABIN969091) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-EGF Antibody (ABIN969091)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Product cited in:

Yacoub, McKinstry, Hinman, Chung, Dent, Hagan: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003

Soubeyran, Kowanetz, Szymkiewicz, Langdon, Dikic: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002

Background publications

Gout, Dhand, Panayotou, Fry, Hiles, Otsu, Waterfield: "Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 395-405, 1993

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN969091) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 1: Immunohistochemical analysis of paraffin-embedded human breast cancer,Lung ...
Immunohistochemistry (IHC) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 2: Immunohistochemical analysis of paraffin-embedded human Uterus tissues usin...
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 3: Western blot analysis using EGF mouse mAb against EGF-hIgGFc transfected HE...