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anti-Human EGF antibody for ELISA

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • AI790464
  • CEGF
  • HOMG4
  • URG
Reactivity
Human
276
105
59
15
12
7
4
3
2
1
Host
Mouse
263
122
29
10
7
3
Clonality
Monoclonal
Conjugate
This EGF antibody is un-conjugated
47
24
21
8
8
8
4
4
4
4
4
4
4
4
4
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
270
268
69
48
46
27
27
22
20
15
11
8
6
5
4
3
2
2
2
2
1
Supplier
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Catalog No. ABIN969091
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012155715 ABIN1724732 ICC ELISA WB Mouse IgG1 Log in to see 4-00E-11 2
0.0012155715 ABIN3044516 ELISA WB Rabbit IgG AA 971-1023 Log in to see Polyclonal
0.0012155715 ABIN574050 IHC (p) ELISA WB Mouse IgG1 Log in to see 9D7F11
0.0012155715 ABIN966030 IF ELISA WB Mouse IgG1 Log in to see 9D7F11 4
0.0012155715 ABIN966031 IHC ELISA WB Mouse IgG1 Log in to see 4E11 4
0.0012155715 ABIN1976358 IHC ELISA WB PE Rabbit IgG AA 689-720 Log in to see Polyclonal
0.0012155715 ABIN1997183 ELISA Rabbit IgG AA 971-1023 Log in to see 8 1
0.0012155715 ABIN2470896 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11
0.0012155715 ABIN1971768 IHC ELISA WB FITC Rabbit IgG AA 689-720 Log in to see Polyclonal
0.0012155715 ABIN205919 Func IHC (p) ELISA WB Rabbit IgG Extracellular Domain Log in to see Polyclonal
0.0012155715 ABIN1965354 IHC ELISA WB APC Rabbit IgG AA 689-720 Log in to see Polyclonal
0.0012155715 ABIN1962977 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 689-720 Log in to see Polyclonal
0.0012155715 ABIN2473398 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 1
0.0012155715 ABIN1824611 ELISA HRP Mouse IgG2a Log in to see KT2
0.0012155715 ABIN467132 IF ELISA Mouse IgG1 Log in to see 4E11
0.0012155715 ABIN1976359 ELISA WB PE Rabbit IgG C-Term, AA 939-967 Log in to see Polyclonal
0.0012155715 ABIN1974098 IHC ELISA WB HRP Rabbit IgG AA 689-720 Log in to see Polyclonal
0.0012155715 ABIN2167745 IF ELISA WB Mouse IgG1 Log in to see 4-00E-011
0.0012155715 ABIN2167746 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11
0.0012155715 ABIN283565 ELISA Mouse IgG1 Log in to see M01102818

Top referenced anti-EGF antibody for ELISA

Similar anti-EGF Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 192 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 18 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 53 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Neutralization (Neut) 14 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human
(276), (105), (59), (15), (12), (7), (4), (3), (2), (1)
Host Mouse
(263), (122), (29), (10), (7), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGF antibody is un-conjugated
(47), (24), (21), (8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(270), (268), (69), (48), (46), (27), (27), (22), (20), (15), (11), (8), (6), (5), (4), (3), (2), (2), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN969091) Images
Purification Ascites
Immunogen Purified recombinant fragment of human EGF expressed in E. coli.
Clone 9D7F11
Isotype IgG1

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF Antibody (ABIN969091) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Molecular Weight 134 kDa
Gene ID 1950
HGNC 1950
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF Antibody (ABIN969091) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF Antibody (ABIN969091) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-EGF Antibody (ABIN969091)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Product cited in:

Yacoub, McKinstry, Hinman, Chung, Dent, Hagan: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003

Soubeyran, Kowanetz, Szymkiewicz, Langdon, Dikic: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002

Background publications

Gout, Dhand, Panayotou, Fry, Hiles, Otsu, Waterfield: "Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 395-405, 1993

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN969091) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 1: Immunohistochemical analysis of paraffin-embedded human breast cancer,Lung ...
Immunohistochemistry (IHC) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 2: Immunohistochemical analysis of paraffin-embedded human Uterus tissues usin...
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 3: Western blot analysis using EGF mouse mAb against EGF-hIgGFc transfected HE...