You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human EGF antibody for ELISA (Capture)

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • AI790464
  • CEGF
  • HOMG4
  • URG
Reactivity
Human
276
105
59
15
12
7
4
3
2
1
Host
Mouse
263
122
29
10
7
3
Clonality
Monoclonal
Conjugate
This EGF antibody is un-conjugated
47
24
21
8
8
8
4
4
4
4
4
4
4
4
4
Application
ELISA (Capture)
271
269
70
48
46
27
27
22
20
15
11
8
6
5
4
3
2
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN114522
$ 522.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008664096 ABIN265192 ELISA (Capture) Rabbit IgG Log in to see Polyclonal

Similar anti-EGF Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 192 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 18 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 53 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Neutralization (Neut) 14 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human
(276), (105), (59), (15), (12), (7), (4), (3), (2), (1)
Host Mouse
(263), (122), (29), (10), (7), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGF antibody is un-conjugated
(47), (24), (21), (8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application ELISA (Capture)
(271), (269), (70), (48), (46), (27), (27), (22), (20), (15), (11), (8), (6), (5), (4), (3), (2), (2), (2), (1), (1)
Supplier Log in to see

Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Specificity This antibody reacts with natural and recombinant human EGF. Does not cross react with other Human cytokines tested such as IL-1 beta, IL-8, MCAF, TGF-beta and SAA.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatograpghy on Protein G.
Immunogen Purified recombinant Human EGF, 6kDa, 53 amino acid residues
Clone S-134
Isotype IgG1

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Gene ID 1950
NCBI Accession NP_001954
UniProt P01133
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes ELISA: This antibody can be used as a Capture antibody in sandwich ELISA applications forHuman EGF detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 0.3 μg/well, Substrate: TMB. If the above suggestedconditions are followed approximately 20 pg/mL of EGF in serum/plasma or 15 pg/mL ofEGF in cell medium/urine can be detected with an assay range of 0-1000 pg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Reconstitution Restore with double distillated water is recommended to adjust the final concentration to 1.00 mg/mL
Concentration 1.00 mg/mL
Buffer 0.01 M PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.