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|Antigen||Epidermal Growth Factor (EGF) Antibodies|
|Conjugate||This EGF antibody is un-conjugated Alternatives|
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Product Details anti-EGF AntibodyTarget Details EGF Application Details Handling Images
|Specificity||This antibody reacts with natural and recombinant human EGF. Does not cross react with other Human cytokines tested such as IL-1 beta, IL-8, MCAF, TGF-beta and SAA.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Purification||Affinity Chromatograpghy on Protein G.|
|Immunogen||Purified recombinant Human EGF, 6kDa, 53 amino acid residues|
Target Details EGFProduct Details anti-EGF Antibody Application Details Handling Images back to top
|Alternative Name||EGF (EGF Antibody Abstract)|
|Background||Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone|
|Research Area||Cancer, Growth Factors|
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation|
Application DetailsProduct Details anti-EGF Antibody Target Details EGF Handling Images back to top
ELISA: This antibody can be used as a Capture antibody in sandwich ELISA applications forHuman EGF detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 0.3 μg/well, Substrate: TMB. If the above suggestedconditions are followed approximately 20 pg/mL of EGF in serum/plasma or 15 pg/mL ofEGF in cell medium/urine can be detected with an assay range of 0-1000 pg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGF Antibody Target Details EGF Application Details Images back to top
|Reconstitution||Restore with double distillated water is recommended to adjust the final concentration to 1.00 mg/mL|
|Buffer||0.01 M PBS, pH 7.2 without preservatives|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.|