anti-Human EGF antibody for Immunohistochemistry

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Reactivity
Human
337
164
88
19
14
7
4
2
2
1
1
1
Host
Mouse
310
150
29
24
6
4
Clonality
Monoclonal
Conjugate
This EGF antibody is un-conjugated
47
27
22
12
12
8
7
7
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
352
288
127
92
54
33
30
28
26
25
19
14
10
7
6
5
4
3
2
2
2
1
1
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Catalog No. ABIN969091
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.385681 ABIN446990 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 3
9.385681 ABIN1872450 IHC WB Rabbit IgG Log in to see Polyclonal
9.385681 ABIN2470896 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11
9.385681 ABIN3222771 IHC WB Rabbit Log in to see Polyclonal
9.385681 ABIN4307155 ELISA IHC IHC (p) WB Mouse IgG1 Log in to see 9D7F11
9.385681 ABIN3030832 IHC ELISA WB Rabbit Ig Fraction AA 690-720 Log in to see Polyclonal
9.385681 ABIN931410 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11
9.385681 ABIN1843510 IHC ELISA WB Mouse IgG1 Log in to see
9.385681 ABIN2405566 IHC WB Rabbit IgG Log in to see Polyclonal
9.385681 ABIN2421523 IHC ELISA Rabbit IgG Log in to see Polyclonal
9.385681 ABIN3016522 IHC WB Rabbit Log in to see Polyclonal
1 ABIN2869465 ELISA IHC WB Mouse IgG1 Log in to see 9D7F11 4
1 ABIN3184415 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1453236 IHC ELISA WB Rabbit IgG AA 660-709 Log in to see Polyclonal
1 ABIN966031 IHC ELISA WB Mouse IgG1 Log in to see 4E11 4
1 ABIN4307157 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN4307158 IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN465856 ELISA IHC Neut WB Biotin Rabbit Log in to see Polyclonal
1 ABIN465547 IHC Neut ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1077992 ICC IHC IP WB Rabbit IgG AA 609-751 Log in to see Polyclonal

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human
(337), (164), (88), (19), (14), (7), (4), (2), (2), (1), (1), (1)
Host Mouse
(310), (150), (29), (24), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGF antibody is un-conjugated
(47), (27), (22), (12), (12), (8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(352), (288), (127), (92), (54), (33), (30), (28), (26), (25), (19), (14), (10), (7), (6), (5), (4), (3), (2), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF antibody (ABIN969091) Images
Purification Ascites
Immunogen Purified recombinant fragment of human EGF expressed in E. Coli.
Clone 9D7F11
Isotype IgG1

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF antibody (ABIN969091) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Molecular Weight 134 kDa
Gene ID 1950
HGNC 1950
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF antibody (ABIN969091) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF antibody (ABIN969091) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-EGF antibody (ABIN969091)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Product cited in:

Yacoub, McKinstry, Hinman, Chung, Dent, Hagan: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003

Soubeyran, Kowanetz, Szymkiewicz, Langdon, Dikic: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002

Background publications

Gout, Dhand, Panayotou, Fry, Hiles, Otsu, Waterfield: "Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 395-405, 1993

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF antibody (ABIN969091) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Epidermal Growth Factor (EGF) antibody (ABIN969091) Figure 1: Immunohistochemical analysis of paraffin-embedded human breast cancer,Lung ...
Immunohistochemistry (IHC) image for anti-Epidermal Growth Factor (EGF) antibody (ABIN969091) Figure 2: Immunohistochemical analysis of paraffin-embedded human Uterus tissues usin...
Western Blotting (WB) image for anti-Epidermal Growth Factor (EGF) antibody (ABIN969091) Figure 3: Western blot analysis using EGF mouse mAb against EGF-hIgGFc transfected HE...