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anti-Human EGF antibody for Immunohistochemistry

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
  • LOC100008808
Reactivity
Human
325
142
84
17
13
7
4
2
1
1
1
Host
Mouse
293
143
30
10
7
4
Clonality
Monoclonal
Conjugate
This EGF antibody is un-conjugated
48
25
22
10
8
8
6
6
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
337
285
109
70
54
32
27
24
20
20
17
14
10
7
6
5
4
2
2
2
2
1
1
1
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Catalog No. ABIN969091
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.72946 ABIN446990 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 3
8.72946 ABIN1872450 IHC WB Rabbit IgG Log in to see Polyclonal
8.72946 ABIN2160822 IHC WB Rabbit Ig Fraction Center Log in to see Polyclonal
8.72946 ABIN2470896 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11
8.72946 ABIN2167746 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11
8.72946 ABIN3222771 IHC WB Rabbit Log in to see Polyclonal
8.72946 ABIN4307155 ELISA IHC IHC (p) WB Mouse IgG1 Log in to see 9D7F11
8.72946 ABIN3030832 IHC ELISA WB Rabbit Ig Fraction AA 690-720 Log in to see Polyclonal
8.72946 ABIN931410 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11
8.72946 ABIN2421523 IHC ELISA Rabbit IgG Log in to see Polyclonal
8.72946 ABIN1843510 IHC ELISA WB Mouse IgG1 Log in to see
8.72946 ABIN2405566 IHC WB Rabbit IgG Log in to see Polyclonal
8.72946 ABIN3016522 IHC WB Rabbit Log in to see Polyclonal
1 ABIN2869465 ELISA IHC WB Mouse IgG1 Log in to see 9D7F11 4
1 ABIN3184415 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN4307157 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN966031 IHC ELISA WB Mouse IgG1 Log in to see 4E11 4
1 ABIN4307158 IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN465856 ELISA IHC Neut WB Biotin Rabbit Log in to see Polyclonal
1 ABIN1858692 ICC IHC IP WB Rabbit IgG AA 971-1023 Log in to see Polyclonal

Top referenced anti-EGF antibody for Immunohistochemistry

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ELISA 205 Antibodies
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Flow Cytometry (FACS) 16 Antibodies
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General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human
(325), (142), (84), (17), (13), (7), (4), (2), (1), (1), (1)
Host Mouse
(293), (143), (30), (10), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGF antibody is un-conjugated
(48), (25), (22), (10), (8), (8), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(337), (285), (109), (70), (54), (32), (27), (24), (20), (20), (17), (14), (10), (7), (6), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN969091) Images
Purification Ascites
Immunogen Purified recombinant fragment of human EGF expressed in E. coli.
Clone 9D7F11
Isotype IgG1

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF Antibody (ABIN969091) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Molecular Weight 134 kDa
Gene ID 1950
HGNC 1950
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF Antibody (ABIN969091) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF Antibody (ABIN969091) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-EGF Antibody (ABIN969091)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Product cited in:

Yacoub, McKinstry, Hinman, Chung, Dent, Hagan: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003

Soubeyran, Kowanetz, Szymkiewicz, Langdon, Dikic: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002

Background publications

Gout, Dhand, Panayotou, Fry, Hiles, Otsu, Waterfield: "Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 395-405, 1993

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN969091) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 1: Immunohistochemical analysis of paraffin-embedded human breast cancer,Lung ...
Immunohistochemistry (IHC) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 2: Immunohistochemical analysis of paraffin-embedded human Uterus tissues usin...
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 3: Western blot analysis using EGF mouse mAb against EGF-hIgGFc transfected HE...