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anti-Human EGF antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • AI790464
  • CEGF
  • HOMG4
  • URG
Epitope
AA 689-720, Middle Region
30
19
14
14
11
10
8
7
5
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
313
123
76
15
13
7
4
3
2
1
Host
Rabbit
281
140
29
11
7
4
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
48
24
22
10
8
8
6
6
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
305
274
91
68
46
41
26
23
20
17
17
14
11
8
6
5
4
2
2
2
2
1
Supplier
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Catalog No. ABIN952036
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34288716 ABIN498560 IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN656805 IHC (p) WB Rabbit Ig AA 690-720, Center Log in to see Polyclonal 1
0.0008982679 ABIN574050 IHC (p) ELISA WB Mouse IgG1 Log in to see 9D7F11
0.0008982679 ABIN115991 EIA IHC (fro) IHC (p) WB Biotin Rabbit Log in to see Polyclonal
0.0008982679 ABIN115992 EIA IHC (fro) IHC (p) WB Biotin Rabbit Log in to see Polyclonal
0.0008982679 ABIN115993 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN115994 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN676613 IF (p) IHC (p) Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN205919 Func IHC (p) ELISA WB Rabbit IgG Extracellular Domain Log in to see Polyclonal
0.0008982679 ABIN3201442 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 609-751 Log in to see
0.0008982679 ABIN1077992 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 609-751 Log in to see Polyclonal
0.0008982679 ABIN1858692 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 971-1023 Log in to see Polyclonal
0.0008982679 ABIN259891 IHC (p) IHC ELISA Mouse IgG1 Log in to see EGF-10
0.0008982679 ABIN1813738 IHC (p) WB Rabbit AA 689-720 Log in to see Polyclonal
0.0008982679 ABIN676615 IHC (p) Biotin Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN676622 IHC (p) HRP Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN1107061 EIA IHC (p) WB Mouse IgG1 Log in to see 9D7F11
0.0008982679 ABIN1385262 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN446990 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 3
0.0008982679 ABIN2473397 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 1

Top referenced anti-EGF antibody for Immunohistochemistry (Paraffin-embedded Sections)

  • Human Polyclonal EGF Primary Antibody for IHC, IHC (p) - ABIN446990 (3 Publications): Sette, Salvati, Memeo, Fecchi, Colarossi, Di Matteo, Signore, Biffoni, DAndrea, De Antoni, Canzonieri, De Maria, Eramo: EGFR inhibition abrogates leiomyosarcoma cell chemoresistance through inactivation of survival pathways and impairment of CSC potential. in PLoS ONE 2012 (PubMed)

Similar anti-EGF Antibodies

Application / Reactivity Mouse (Murine) Human
Blocking Antibody (Inhibition) 1 Antibodies 3 Antibodies
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 40 Antibodies 194 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 5 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 90 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope AA 689-720, Middle Region
(30), (19), (14), (14), (11), (10), (8), (7), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(313), (123), (76), (15), (13), (7), (4), (3), (2), (1)
Host Rabbit
(281), (140), (29), (11), (7), (4)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(48), (24), (22), (10), (8), (8), (6), (6), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(305), (274), (91), (68), (46), (41), (26), (23), (20), (17), (17), (14), (11), (8), (6), (5), (4), (2), (2), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN952036) Images
Specificity This antibody reacts to EGF.
Cross-Reactivity (Details) Species reactivity (tested):Human and Mouse.
Purification Affinity chromatography on Protein A
Immunogen KLH conjugated synthetic peptide between 689-720 amino acids from the Central region of human EGF
Isotype Ig

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF Antibody (ABIN952036) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Molecular Weight 133994 Da
Gene ID 1950
NCBI Accession NP_001954
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF Antibody (ABIN952036) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF Antibody (ABIN952036) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-EGF Antibody (ABIN952036)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Background publications

Medts, de Diesbach, Cominelli, NKuli, Tyteca, Courtoy: "Acute ligand-independent Src activation mimics low EGF-induced EGFR surface signalling and redistribution into recycling endosomes." in: Experimental cell research, Vol. 316, Issue 19, pp. 3239-53, 2010

Xu, Jiang, Steed, Davidge, Fu: "TGFβ and EGF synergistically induce a more invasive phenotype of epithelial ovarian cancer cells." in: Biochemical and biophysical research communications, Vol. 401, Issue 3, pp. 376-81, 2010

Lupien, Meyer, Bailey, Eeckhoute, Cook, Westerling, Zhang, Carroll, Rhodes, Liu, Brown: "Growth factor stimulation induces a distinct ER(alpha) cistrome underlying breast cancer endocrine resistance." in: Genes & development, Vol. 24, Issue 19, pp. 2219-27, 2010

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN952036) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGF antibody (Epidermal Growth Factor) (AA 689-720) (ABIN952036) EGF Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin em...
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (AA 689-720) (ABIN952036) EGF Antibody (Center) western blot analysis in mouse stomach tissue lysates (35µg/lan...