anti-Human EGF antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
  • LOC100008808
Epitope
AA 689-720, Middle Region
27
16
15
12
11
11
10
8
8
8
8
7
5
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
324
142
87
19
14
7
4
2
2
1
1
1
Host
Rabbit
296
143
29
10
6
4
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
46
24
21
10
8
8
6
6
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
334
280
108
75
54
33
26
24
19
19
17
14
10
7
6
5
4
2
2
2
2
1
1
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Catalog No. ABIN952036
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.878013 ABIN446990 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 3
13.878013 ABIN498560 IHC (p) WB Rabbit Log in to see Polyclonal
13.878013 ABIN4307158 IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
13.878013 ABIN4307157 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
13.878013 ABIN4307155 ELISA IHC IHC (p) WB Mouse IgG1 Log in to see 9D7F11
1 ABIN656805 IHC (p) WB Rabbit Ig AA 690-720, Center Log in to see Polyclonal 1
1 ABIN574050 IHC (p) ELISA WB Mouse IgG1 Log in to see 9D7F11
1 ABIN115991 EIA IHC (fro) IHC (p) WB Biotin Rabbit Log in to see Polyclonal
1 ABIN115992 EIA IHC (fro) IHC (p) WB Biotin Rabbit Log in to see Polyclonal
1 ABIN115993 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN115994 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN3201442 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 609-751 Log in to see
1 ABIN205919 Func IHC (p) ELISA WB Rabbit IgG Extracellular Domain Log in to see Polyclonal
1 ABIN2473397 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN676613 IF (p) IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN1385262 IF (p) IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN259891 IHC (p) IHC ELISA Mouse IgG1 Log in to see EGF-10
1 ABIN1956981 ELISA IHC (p) WB FITC Rabbit IgG Log in to see Polyclonal
1 ABIN1958088 ELISA IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN1955747 ELISA IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal

Top referenced anti-EGF antibody for Immunohistochemistry (Paraffin-embedded Sections)

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Application / Reactivity Mouse (Murine) Human
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ELISA 47 Antibodies
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Immunocytochemistry (ICC) 2 Antibodies
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Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 41 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunoprecipitation (IP) 2 Antibodies
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Western Blotting (WB) 103 Antibodies
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General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope AA 689-720, Middle Region
(27), (16), (15), (12), (11), (11), (10), (8), (8), (8), (8), (7), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(324), (142), (87), (19), (14), (7), (4), (2), (2), (1), (1), (1)
Host Rabbit
(296), (143), (29), (10), (6), (4)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(46), (24), (21), (10), (8), (8), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(334), (280), (108), (75), (54), (33), (26), (24), (19), (19), (17), (14), (10), (7), (6), (5), (4), (2), (2), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling ProductDetails: References for anti-EGF antibody (ABIN952036) Images
Specificity This antibody reacts to EGF.
Cross-Reactivity (Details) Species reactivity (tested):Human and Mouse.
Purification Affinity chromatography on Protein A
Immunogen KLH conjugated synthetic peptide between 689-720 amino acids from the Central region of human EGF
Isotype Ig

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling ProductDetails: References for anti-EGF antibody (ABIN952036) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Molecular Weight 133994 Da
Gene ID 1950
NCBI Accession NP_001954
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling ProductDetails: References for anti-EGF antibody (ABIN952036) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details ProductDetails: References for anti-EGF antibody (ABIN952036) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-EGF antibody (ABIN952036)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Background publications

Medts, de Diesbach, Cominelli, NKuli, Tyteca, Courtoy: "Acute ligand-independent Src activation mimics low EGF-induced EGFR surface signalling and redistribution into recycling endosomes." in: Experimental cell research, Vol. 316, Issue 19, pp. 3239-53, 2010

Xu, Jiang, Steed, Davidge, Fu: "TGFβ and EGF synergistically induce a more invasive phenotype of epithelial ovarian cancer cells." in: Biochemical and biophysical research communications, Vol. 401, Issue 3, pp. 376-81, 2010

Lupien, Meyer, Bailey, Eeckhoute, Cook, Westerling, Zhang, Carroll, Rhodes, Liu, Brown: "Growth factor stimulation induces a distinct ER(alpha) cistrome underlying breast cancer endocrine resistance." in: Genes & development, Vol. 24, Issue 19, pp. 2219-27, 2010

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling ProductDetails: References for anti-EGF antibody (ABIN952036) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGF antibody (Epidermal Growth Factor) (AA 689-720) (ABIN952036) EGF Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin em...
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (AA 689-720) (ABIN952036) EGF Antibody (Center) western blot analysis in mouse stomach tissue lysates (35µg/lan...