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|Application / Reactivity||Mouse (Murine)|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|Enzyme Immunoassay (EIA)||9 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||31 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Neutralization (Neut)||4 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Western Blotting (WB)||88 Antibodies|
|Antigen||Epidermal Growth Factor (EGF) Antibodies|
|Epitope||AA 977-1029 Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This EGF antibody is un-conjugated Alternatives|
ELISA, Western Blotting (WB)
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Product Details anti-EGF AntibodyTarget Details EGF Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(EGF) detection. Tested with WB, ELISA in Mouse.
Gene Name: epidermal growth factor
Protein Name: Pro-epidermal growth factor
|Purification||Immunogen affinity purified.|
|Immunogen||E. coli-derived mouse EGF recombinant protein (Position: N977-R1029). Mouse EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and rat EGF.|
Target Details EGFProduct Details anti-EGF Antibody Application Details Handling Images back to top
|Alternative Name||EGF (EGF Antibody Abstract)|
|Background||EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.|
|Research Area||Cancer, Growth Factors|
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-EGF Antibody Target Details EGF Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGF Antibody Target Details EGF Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-EGF Antibody Target Details EGF Application Details Handling back to top