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anti-Mouse (Murine) EGF antibody for Blocking Antibody

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • AI790464
  • CEGF
  • HOMG4
  • URG
Reactivity
Mouse (Murine)
277
104
59
15
12
7
4
3
2
1
Host
Goat
263
123
28
10
7
3
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
47
24
21
8
8
8
4
4
4
4
4
4
4
4
4
Application
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
270
268
70
48
46
27
27
22
20
15
11
8
5
5
4
3
2
2
2
2
1
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Catalog No. ABIN1002256
$ 374.22
Plus shipping costs $45.00

Similar anti-EGF Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 40 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 5 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 73 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Mouse (Murine)
(277), (104), (59), (15), (12), (7), (4), (3), (2), (1)
Host Goat
(263), (123), (28), (10), (7), (3)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(47), (24), (21), (8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
(270), (268), (70), (48), (46), (27), (27), (22), (20), (15), (11), (8), (5), (5), (4), (3), (2), (2), (2), (2), (1)
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Purification Antigen affinity purified polyclol antibody
Sterility Sterile filtered
Immunogen recombint mEGF (murine EGF)
Isotype IgG

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name Epidermal Growth Factor (EGF Antibody Abstract)
Background Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mEGF (murine EGF). Anti-Murine EGF specific antibody was purified by affinity chromatography employing immobilized mEGF matrix.
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes To yield one-half maximal inhibition [ND₅₀] of the biological activity of mEGF (0.1 ng/µl), a concentration of 3.0 - 5.0 ng/µl of this antibody is required. To detect mEGF by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEGF. To detect mEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Murine EGF (PK-AB815-60170GMB) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEGF. To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunihostochemistry: NA.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Format Lyophilized
Buffer A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
Handling Advice The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
Storage -20 °C
Expiry Date 24 months