anti-Mouse (Murine) EGF antibody for Neutralization

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
  • LOC100008808
Reactivity
Mouse (Murine)
340
163
87
19
14
7
4
2
2
1
1
1
Host
Goat
309
153
28
24
6
4
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
47
27
22
12
10
8
7
7
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
Application
Neutralization (Neut), ELISA, Western Blotting (WB)
353
289
129
92
54
32
30
28
26
25
19
14
10
7
6
5
4
3
2
2
2
1
1
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Available images

Catalog No. ABIN465058
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN465077 ELISA Neut WB Biotin Goat Log in to see Polyclonal
1 ABIN2257865 Neut ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN116365 EIA Neut WB Goat Log in to see Polyclonal
1 ABIN116366 EIA Neut WB Goat Log in to see Polyclonal

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Mouse (Murine)
(340), (163), (87), (19), (14), (7), (4), (2), (2), (1), (1), (1)
Host Goat
(309), (153), (28), (24), (6), (4)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(47), (27), (22), (12), (10), (8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2)
Application Neutralization (Neut), ELISA, Western Blotting (WB)
(353), (289), (129), (92), (54), (32), (30), (28), (26), (25), (19), (14), (10), (7), (6), (5), (4), (3), (2), (2), (2), (1), (1)
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Specificity Mouse EGF
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Murine EGF.

Type of Immunogen: Cell extract

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Name/Gene ID: EGF

Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor
Gene ID 1950
UniProt P01133
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes Approved: ELISA (0.5 - 2 μg/mL), Neut, WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Murine EGF (0.1 ng/mL), a concentration of 3-5 ng/mL of this antibody is required. ELISA: To detect mEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine EGF (LS-C104401) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF. Western Blot: To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Format Lyophilized
Reconstitution Sterile water
Concentration Lot specific
Buffer Lyophilized from PBS, pH 7.2
Handling Advice Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling back to top
Supplier Images
 image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465058) anti-Epidermal Growth Factor (EGF) antibody
 image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465058) anti-Epidermal Growth Factor (EGF) antibody (Image 2)
 image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465058) anti-Epidermal Growth Factor (EGF) antibody (Image 3)