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|Application / Reactivity||Rat (Rattus)||Human||Mouse (Murine)|
|Western Blotting (WB)||48 Antibodies||216 Antibodies||90 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies||2 Antibodies|
|Neutralization (Neut)||5 Antibodies||28 Antibodies||8 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||10 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||43 Antibodies||34 Antibodies||28 Antibodies|
|Immunohistochemistry (IHC)||72 Antibodies||31 Antibodies||31 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies||7 Antibodies|
|Antigen||Epidermal Growth Factor (EGF) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This EGF antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-EGF AntibodyTarget Details EGF Application Details Handling Images
|Specificity||This antibody detects endogenous levels of EGF protein. (region surrounding Arg709)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details EGFProduct Details anti-EGF Antibody Application Details Handling Images back to top
|Alternative Name||EGF (EGF Antibody Abstract)|
|Background||Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid protein of 6 kDa originally found in rodents and humans. EGF has been shown to be a potent mitogen for a variety of cell types both in vivo and in vitro. It binds to the 170 kDa EGF receptor on the surface of cells and mediates intrinsic phos phoryl ation of the receptor on tyrosine residues. EGF has been detected in nearly all body fluids such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF, TGFalpha and vaccinia virus growth factor exhibit 30-40 % amino acid homology. Several additional members of the EGF/TGF family have been described, these include Cripto, Amphiregulin and the heparin-binding EGF-like growth factor, each of which, except Cripto, binds to the EGF receptor.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone|
|Molecular Weight||approx. 130 kDa|
|Research Area||Cancer, Growth Factors|
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-EGF Antibody Target Details EGF Handling Images back to top
Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGF Antibody Target Details EGF Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-EGF Antibody Target Details EGF Application Details Handling back to top