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anti-Rat (Rattus) EGF antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • AI790464
  • CEGF
  • HOMG4
  • URG
Reactivity
Human, Mouse (Murine), Rat (Rattus)
311
121
74
15
13
7
4
3
2
1
Host
Rabbit
280
139
29
10
7
4
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
48
24
22
10
8
8
6
6
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
303
273
89
66
46
41
27
23
20
17
17
14
11
8
6
5
4
2
2
2
2
1
Supplier
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Catalog No. ABIN498560
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008985059 ABIN1385702 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
0.0008985059 ABIN676643 IF (p) IHC (p) Rabbit IgG Log in to see Polyclonal
0.0008985059 ABIN676652 IHC (p) HRP Rabbit IgG Log in to see Polyclonal
0.0008985059 ABIN676645 IHC (p) Biotin Rabbit IgG Log in to see Polyclonal
0.0008985059 ABIN1414185 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
0.0008985059 ABIN1396308 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
0.0008985059 ABIN1385701 IF (p) IHC (p) WB Mouse IgG Log in to see Polyclonal
0.0008985059 ABIN1077994 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 974-1026 Log in to see Polyclonal
0.0008985059 ABIN446990 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal
0.0008985059 ABIN3201446 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
0.0008985059 ABIN4307160 IHC IHC (p) WB Allophycocyanin Rabbit Internal Region Log in to see Polyclonal
0.0008985059 ABIN4307161 IHC IHC (p) WB Biotin Rabbit Internal Region Log in to see Polyclonal
0.0008985059 ABIN4307162 IHC IHC (p) WB DyLight 350 Rabbit Internal Region Log in to see Polyclonal
0.0008985059 ABIN4307163 IHC IHC (p) WB DyLight 405 Rabbit Internal Region Log in to see Polyclonal
0.0008985059 ABIN4307166 IHC IHC (p) WB DyLight 550 Rabbit Internal Region Log in to see Polyclonal
0.0008985059 ABIN4307168 IHC IHC (p) WB DyLight 680 Rabbit Internal Region Log in to see Polyclonal
0.0008985059 ABIN4307170 IHC IHC (p) WB HRP Rabbit Internal Region Log in to see Polyclonal
0.0008985059 ABIN4307171 IHC IHC (p) WB PE Rabbit Internal Region Log in to see Polyclonal
0.0008985059 ABIN4307173 IHC IHC (p) WB Alexa Fluor 488 Rabbit Internal Region Log in to see Polyclonal
0.0008985059 ABIN4307164 IHC IHC (p) WB DyLight 405LS Rabbit Internal Region Log in to see Polyclonal

Top referenced anti-EGF antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-EGF Antibodies

Application / Reactivity Rat (Rattus) Human Mouse (Murine)
Western Blotting (WB) 48 Antibodies 216 Antibodies 90 Antibodies
Radioimmunoassay (RIA) 2 Antibodies 2 Antibodies
Neutralization (Neut) 5 Antibodies 28 Antibodies 8 Antibodies
Immunoprecipitation (IP) 2 Antibodies 10 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies 34 Antibodies 28 Antibodies
Immunohistochemistry (IHC) 72 Antibodies 31 Antibodies 31 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 7 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(311), (121), (74), (15), (13), (7), (4), (3), (2), (1)
Host Rabbit
(280), (139), (29), (10), (7), (4)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(48), (24), (22), (10), (8), (8), (6), (6), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(303), (273), (89), (66), (46), (41), (27), (23), (20), (17), (17), (14), (11), (8), (6), (5), (4), (2), (2), (2), (2), (1)
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Specificity This antibody detects endogenous levels of EGF protein. (region surrounding Arg709)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid protein of 6 kDa originally found in rodents and humans. EGF has been shown to be a potent mitogen for a variety of cell types both in vivo and in vitro. It binds to the 170 kDa EGF receptor on the surface of cells and mediates intrinsic phos phoryl ation of the receptor on tyrosine residues. EGF has been detected in nearly all body fluids such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF, TGFalpha and vaccinia virus growth factor exhibit 30-40 % amino acid homology. Several additional members of the EGF/TGF family have been described, these include Cripto, Amphiregulin and the heparin-binding EGF-like growth factor, each of which, except Cripto, binds to the EGF receptor.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Molecular Weight approx. 130 kDa
Gene ID 1950
NCBI Accession NP_001954
UniProt P01133
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN498560) Immunohistochemistry (IHC) analysis of EGF antibody in paraffin-embedded human...
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN498560) Western blot analysis of EGF in extracts from NIH-3T3 cells using anti-EGF ant...