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Rabbit EGF ELISA Kit

Recommended EGF Product (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) ELISA Kits
  • AI790464
  • CEGF
  • HOMG4
  • URG
Reactivity
Rabbit
Alternatives
Kits with alternative reactivity to:
40
28
22
9
5
4
4
4
2
2
2
1
1
Methode Type
Competition ELISA
Detection Range
0.5-8 ng/mL
Minimum Detection Limit
0.5 ng/mL
Application
ELISA
Supplier
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Catalog No. ABIN365535
$ 812.78
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012117105 ABIN1561819 Sandwich ELISA Serum, Plasma, Biological Fluids Log in to see
0.0012117105 ABIN510262 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012117105 ABIN991499 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012117105 ABIN2108594 Sandwich ELISA Log in to see
0.0012117105 ABIN2533050 Serum, Plasma, Biological Fluids Log in to see

Similar EGF ELISA Kits

Application / Reactivity Rabbit
ELISA 6 ELISA Kits

General

Antigen Epidermal Growth Factor (EGF) ELISA Kits
Reactivity Rabbit
Kits with alternative reactivity to:
(40), (28), (22), (9), (5), (4), (4), (4), (2), (2), (2), (1), (1)
Methode Type Competition ELISA
Detection Range 0.5-8 ng/mL
Minimum Detection Limit 0.5 ng/mL
Application ELISA
Supplier Log in to see

Product Details EGF ELISA Kit

Target details Application Details Handling Images
Purpose This immunoassay kit allows for the in vitro rapid detection of rabbit EGF concentrations in serum, plasma, tissue homogenates.
Sample Type Serum, Plasma, Tissue Homogenate
Detection Method Colorimetric
Specificity This assay recognizes rabbit EGF.
Cross-Reactivity (Details) Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
Sensitivity 0.25 ng/ml.
Characteristics The standard curve concentrations used for the ELISA’s were 8 ng/ml, 4 ng/ml, 2 ng/ml, 1 ng/ml, 0.5 ng/ml 3
Material not included
  • Microplate reader capable of measuring absorbance at 450nm, with the correction wavelength set at 540nm or 570nm.
  • An incubator which can provide stable incubation conditions up to 37°C ± 0.5°C.
  • Squirt bottle, manifold dispenser or automated microplate washer.
  • Absorbent paper for blotting the microtiter plate.
  • 100mL and 500mL graduated cylinders.
  • Deionized or distilled water.
  • Pipettes and pipette tips.
  • Test tubes for dilution.

Target details

Product Details EGF ELISA Kit Application Details Handling Images back to top
Antigen
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details EGF ELISA Kit Target details Handling Images back to top
Application Notes
  • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
  • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
  • Grossly hemolyzed samples are not suitable for use in this assay.
  • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
  • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
  • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
  • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
  • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
  • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
Comment

Detection wavelength: 450 nm

Information on standard material:
Some standards are from natural resource, some are recombinant protein, but the recombinant protein will not expressed from Baculovirus. Generally it's CHO cells, but that depends.The formulation of auxiliary material in the standard is classified, but it dosen't contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

Information on reagents:
Most of the stop solution are 1 N H2SO4, a few is not. The formulation of wash solution is classified. None of the components contains (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. Some could contain BSA. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

Information on antibodies:
The provided antibodies and their host vary in different kits. Some are affinity purified, some are Protein A or G purified

Sample Volume 50 μL
Assay Time 1 - 4.5 h
Plate Pre-coated,Strips (12 x 8)
Protocol This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to EGF has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated EGF and incubated. A competitive inhibition reaction is launched between EGF (Standards or samples) and Biotin-conjugated EGF with the pre-coated antibody specific for EGF. The more amount of EGF in samples, the less antibody bound by Biotin-conjugated EGF. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of EGF in the sample. The color development is stopped and the intensity of the color is measured.
Sample Collection
  • Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
  • Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
  • Tissue Homogenates: Rinse 100mg tissue with 1× PBS, homogenize in 1mL of 1× PBS and store overnight at -20°C. After two freeze-thaw cycles to break the cell membranes, centrifuge the homogenates for 5 minutes at 5000 × g, 2-8°C. Remove and assay the supernate immediately. Alternatively, aliquot and store samples at -20°C or -80°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
Assay Precision Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
  • Intra-assay: CV% less than 8%
  • Inter-assay: CV% less than 10%
Restrictions For Research Use only

Handling

Product Details EGF ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Handling Advice
  • The kit should not be used beyond the expiration date on the kit label.
  • Do not mix or substitute reagents with those from other lots or sources.
  • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
  • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
  • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
Storage 4 °C/-20 °C
Storage Comment May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.
Expiry Date 6 months