Rhesus Monkey EGF ELISA Kit

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Antigen
Epidermal Growth Factor (EGF) ELISA Kits
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
  • LOC100008808
Reactivity
Human, Rhesus Monkey
Alternatives
Kits with alternative reactivity to:
45
34
25
10
5
5
4
4
2
2
2
1
1
Method Type
Sandwich ELISA
Detection Range
8-2.000 pg/mL
Minimum Detection Limit
8 pg/mL
Application
ELISA
Options
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Catalog No. ABIN2748060
$ 476.30
Plus shipping costs $45.00

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General

Antigen Epidermal Growth Factor (EGF) ELISA Kits
Reactivity Human, Rhesus Monkey
Kits with alternative reactivity to:
(45), (34), (25), (10), (5), (5), (4), (4), (2), (2), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 8-2.000 pg/mL
Minimum Detection Limit 8 pg/mL
Application ELISA
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Product Details EGF ELISA Kit

Target details Application Details Handling Images
Purpose Rhesus Monkey EGF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA antibody pair detects Rhesus Monkey EGF and human EGF.
Sensitivity 8 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details EGF ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name EGF (EGF ELISA Kit Abstract)
Background EGF-like module-containing mucin-like hormone receptor-like 2 (EGF-like module receptor 2) (CD antigen CD312)
Gene ID 654420
UniProt Q2Q426
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details EGF ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples3 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details EGF ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

Images

Product Details EGF ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for EGF ELISA Kit (Epidermal Growth Factor) (ABIN2748060) Epidermal Growth Factor (EGF) ELISA Kit