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Rhesus Monkey EGF ELISA Kit

Recommended EGF Product (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) ELISA Kits
  • AI790464
  • CEGF
  • HOMG4
  • URG
Reactivity
Human, Rhesus Monkey
Alternatives
Kits with alternative reactivity to:
39
28
22
9
6
4
4
4
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
8-2000 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN2748060
$ 467.50
Plus shipping costs $45.00

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Application / Reactivity Human Rhesus Monkey
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General

Antigen Epidermal Growth Factor (EGF) ELISA Kits
Reactivity Human, Rhesus Monkey
Kits with alternative reactivity to:
(39), (28), (22), (9), (6), (4), (4), (4), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 8-2000 pg/mL
Application ELISA
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Product Details EGF ELISA Kit

Target details Application Details Handling Images
Sample Type Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids
Detection Method Colorimetric
Specificity This ELISA antibody pair detects Rhesus Macaque EGF and human EGF.
Sensitivity 8 pg/ml
Characteristics Quantitative
Components 96-well pre-coated strip plate (12x8)
Wash Buffer Concentrate (20x)
Standard
Sample Diluent Buffers
Biotin-labeled Detection Antibody
HRP-Streptavidin concentrate
TMB One-Step Substrate Reagent
Stop Solution
Cell Lysis Buffer (for cell/tissue lysate kits only)
Material not included Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details EGF ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name EGF (EGF ELISA Kit Abstract)
Background EGF-like module-containing mucin-like hormone receptor-like 2 (EGF-like module receptor 2) (CD antigen CD312)
Gene ID 654420
UniProt Q2Q426
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details EGF ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Plate 96 wells
Assay Procedure Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details EGF ELISA Kit Target details Application Details Images back to top
Storage 4 °C,-20 °C,-80 °C
Storage Comment May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.